Adssl1 (NM_007421) Mouse Untagged Clone

CAT#: MC216097

Adssl1 (untagged) - Mouse adenylosuccinate synthetase like 1 (Adssl1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adssl1
• Synonyms: Adss; Adss1; AI528595
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216097 representing NM_007421
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGGACCCGAGCCTCCAACGACCGGCCCCCCGGCACAGGCGGAGTCAAGAGGGGGCGGCTGCAGC
  AGGAGGCGGCGGCGACAGGCTCCCGCGTGACCGTGGTGCTGGGCGCGCAGTGGGGGGACGAGGGCAAAGG
  CAAGGTGGTGGACCTGCTGGCCACGGACGCCGACATCGTCAGTCGCTGCCAGGGGGGCAACAATGCGGGG
  CACACAGTAGTGGTGGACGGCAAGGAGTATGACTTTCACCTGCTCCCCAGCGGCATCATCAACACCAAGG
  CCGTGTCATTCATTGGCAATGGCGTGGTCATCCACTTGCCGGGCTTGTTTGAAGAGGCAGAGAAGAACGA
  GAAGAAAGGGCTGAAGGACTGGGAGAAGCGTCTCATCATCTCTGACCGGGCTCACCTTGTGTTCGACTTC
  CACCAGGCAGTGGACGGGCTGCAGGAAGTACAACGTCAAGCACAAGAGGGGAAGAATATCGGCACCACCA
  AGAAGGGAATCGGACCAACTTACTCCTCCAAAGCTGCCCGGACAGGCCTCCGCATCTGTGACCTTCTCTC
  GGATTTTGACGAGTTTTCTGCCAGATTCAAGAACCTGGCCCACCAGCACCAGTCCATGTTCCCCACCCTG
  GAAATAGATGTGGAAGGTCAACTCAAAAGGCTCAAGGGCTTTGCTGAGCGCATCAGACCCATGGTTCGAG
  ATGGTGTCTACTTCATGTATGAAGCCCTGCATGGTCCCCCCAAAAAGGTCCTGGTGGAAGGTGCCAACGC
  GGCCCTGCTCGACATCGATTTCGGGACCTACCCCTTTGTGACTTCTTCCAACTGCACTGTGGGTGGTGTG
  TGCACCGGCCTGGGCATCCCGCCCCAGAACATAGGCGATGTGTATGGTGTGGTAAAGGCCTACACCACCC
  GTGTGGGCATTGGGGCCTTCCCCACGGAGCAGATCAATGAAATTGGAGATCTGCTGCAGAACCGTGGCCA
  TGAGTGGGGGGTGACCACAGGCAGGAAGAGGCGCTGCGGCTGGCTGGACCTGATGATCCTCCGATACGCT
  CACATGGTCAATGGCTTCACTGCGCTGGCATTGACCAAGCTGGACATACTGGATGTCCTGAGCGAGATTA
  AAGTTGGCATCTCCTACAAGCTCAACGGAAAGAGGATTCCCTACTTCCCTGCTAACCAGGAGATCCTGCA
  GAAGGTGGAGGTCGAGTATGAAACACTGCCTGGATGGAAAGCAGACACCACAGGCGCCAGGAAGTGGGAG
  GACCTGCCTCCGCAGGCCCAAAGCTACGTGCGCTTCGTGGAGAATCACATGGGTGTTGCAGTCAAATGGG
  TCGGCGTTGGGAAGTCCAGAGAGTCCATGATCCAGCTTTTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007421
• Insert Size: 1374 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007421.2, NP_031447.1
• RefSeq Size: 1806 bp
• RefSeq ORF: 1374 bp
• Locus ID: 11565
• UniProt ID: P28650
• Cytogenetics: 12 F1
• Gene Summary: Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.[UniProtKB/Swiss-Prot Function]