A1cf (NM_001081074) Mouse Tagged ORF Clone

CAT#: MG224176

A1cf (tGFP-tagged) - Mouse APOBEC1 complementation factor (A1cf), (10ug)

Product Images

Circular map for MG224176

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: A1cf
• Synonyms: 1810073H04Rik; Acf; ACF64; ACF65; ASP; MCM; mer; MerCreMer; Tg(Myh6-cre/Esr1*)1Jmk
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224176 representing NM_001081074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCAAATCACAAATCCGGGGATGGATTGAGCGGCACCCAGAAGGAAGCGGCACTCCGCGCACTGG
  TCCAGCGCACAGGATATAGCTTGGTCCAGGAAAATGGACAAAGGAAGTATGGTGGCCCTCCACCAGGCTG
  GGATTCTACACCCCCAGAAAGGGGCTGCGAGATTTTCATTGGGAAACTTCCCCGGGACCTCTTTGAGGAT
  GAACTCATACCATTGTGTGAAAAAATTGGTAAAATTTATGAAATGAGATTGATGATGGATTTTAATGGTA
  ACAACAGAGGCTATGCATTTGTAACATTCTCAAATAAGCAGGAAGCCAAGAATGCAATCAAGCAACTTAA
  TAATTATGAAATTCGGACTGGCCGTCTCTTGGGAGTCTGTGCCAGTGTGGACAATTGCCGATTGTTTGTG
  GGAGGAATCCCCAAAACCAAAAAGAGGGAAGAAATCTTATCAGAGATGAAAAAAGTCACAGAAGGAGTTG
  TTGATGTCATTGTCTACCCAAGTGCTGCTGATAAAACCAAAAACCGGGGATTTGCCTTTGTGGAATATGA
  GAGTCACCGAGCAGCCGCCATGGCTAGGCGGAGGCTGCTGCCAGGAAGAATTCAGTTGTGGGGACATCCT
  ATTGCAGTAGACTGGGCAGAACCAGAAGTTGAAGTTGATGAGGACACAATGTCTTCCGTGAAAATCCTGT
  ACGTAAGGAACCTTATGCTGTCTACCTCGGAAGAAATGATTGAAAAGGAATTCAACAGTATTAAACCAGG
  TGCTGTGGAAAGAGTGAAGAAGATCCGAGACTACGCTTTTGTGCACTTCAGTAACCGAGAAGATGCAGTT
  GAAGCCATGAAGGCTTTGAATGGCAAGGTGCTGGATGGTTCCCCAATAGAAGTGACCTTGGCCAAGCCAG
  TGGACAAGGACAGTTATGTTAGGTACACCCGGGGCACCGGTGGCAGGAACACCATGCTGCAAGGAGAATA
  CACCTACCCTCTGAGCCATGTTTATGACCCTACCACAACCTACCTTGGAGCTCCTGTCTTCTATACCCCC
  CAAGCCTATGCAGCCATTCCAAGTCTTCATTTCCCAGCTACTAAAGGACATCTCAGCAACAGGGCTCTCA
  TCAGGACCCCTTCTGTCAGAGAAATTTACATGAATGTCCCTGTAGGGGCTGCGGGGGTGAGAGGACTGGG
  CGGCCGTGGGTATTTGGCATACACAGGCCTGGGTCGAGGATACCACGTCAAAGGAGACAAGAGAGAAGAC
  AAACTCTATGATCTTCTGCCTGGGATGGAGCTCACCCCGATGAATACTGTCTCTTTAAAACCACAAGGAA
  TTAAACTTGCTCCTCAGATATTAGAAGAAATCTGTCAGAAAAATAACTGGGGACAGCCAGTGTACCAACT
  GCATTCTGCCATTGGACAAGATCAAAGACAGTTATTCCTCTACAAAGTAACTATCCCAGCGCTGGCCAGC
  CAGAATCCTGCAATCCACCCTTTCATACCACCAAAGCTAAGCGCCTATGTGGATGAAGCGAAGAGGTATG
  CTGCAGAGCACACTCTACAGACACTAGGCATCCCCACAGAAGGAGGGGACGCTGGGACTACAGCACCCAC
  GGCCACTTCTGCCACTGTGTTCCCAGGATATGCTGTCCCCAGTGCCACCGCTCCTGTGTCTACAGCCCAG
  CTCAAGCAAGCAGTGACACTTGGACAAGACTTAGCAGCATATACAACCTATGAGGTCTACCCTACTTTTG
  CACTGACCACCCGAGGCGATGCATATGGAACCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224176 representing NM_001081074
  Red=Cloning site Green=Tags(s)
  
  MESNHKSGDGLSGTQKEAALRALVQRTGYSLVQENGQRKYGGPPPGWDSTPPERGCEIFIGKLPRDLFED
  ELIPLCEKIGKIYEMRLMMDFNGNNRGYAFVTFSNKQEAKNAIKQLNNYEIRTGRLLGVCASVDNCRLFV
  GGIPKTKKREEILSEMKKVTEGVVDVIVYPSAADKTKNRGFAFVEYESHRAAAMARRRLLPGRIQLWGHP
  IAVDWAEPEVEVDEDTMSSVKILYVRNLMLSTSEEMIEKEFNSIKPGAVERVKKIRDYAFVHFSNREDAV
  EAMKALNGKVLDGSPIEVTLAKPVDKDSYVRYTRGTGGRNTMLQGEYTYPLSHVYDPTTTYLGAPVFYTP
  QAYAAIPSLHFPATKGHLSNRALIRTPSVREIYMNVPVGAAGVRGLGGRGYLAYTGLGRGYHVKGDKRED
  KLYDLLPGMELTPMNTVSLKPQGIKLAPQILEEICQKNNWGQPVYQLHSAIGQDQRQLFLYKVTIPALAS
  QNPAIHPFIPPKLSAYVDEAKRYAAEHTLQTLGIPTEGGDAGTTAPTATSATVFPGYAVPSATAPVSTAQ
  LKQAVTLGQDLAAYTTYEVYPTFALTTRGDAYGTF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081074
• ORF Size: 1785 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081074.2
• RefSeq Size: 3828 bp
• RefSeq ORF: 1788 bp
• Locus ID: 69865
• UniProt ID: Q5YD48
• Cytogenetics: 19 C1
• Gene Summary: Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also seems to protect the edited APOB mRNA from nonsense-mediated decay (By similarity).[UniProtKB/Swiss-Prot Function]