Atp10d (NM_153389) Mouse Tagged ORF Clone

CAT#: MG223765

Atp10d (tGFP-tagged) - Mouse ATPase class V type 10D (Atp10d), (10ug)

Product Images

Circular map for MG223765

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atp10d
• Synonyms: 9830145H18Rik; ATPVD; D5Buc24e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223765 representing NM_153389
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAATGGCTGGCTTGGAAGCGTAACTCTCCCCGCCCTTTGGATCTCTGCTCCGTTCTCTTTTGGCA
  CTTTGGGATCCGAGGTAACACCATGCTGGGATGAGCGGCCCGGGAGGGACAGATCGCCTGAGCCAGCCGG
  GTCTCCCTGCGTCTTGGGCATGACTGAGCTGCTGCAGTGGGCCAGACATCACTGGCGTCGGCTGAGCCAT
  GGGAGAGCCCAGGGTGAAGATGAGAGGCCATACAACTACGCCTCCCTGCTGGCCTGTGGGGGCAAGTCCT
  CGAGGACCCCCAGACCTGCAGGAAAGCACCGCGTCGTTATTCCCCACCTTCAGTGCTTCAAAGATGAGTA
  CGAAAGGTTTTCTGGAACCTACGTGAATAACCGGATACGGACGACCAAGTACACACTCCTGAACTTTGTG
  CCAAGAAACTTATTTGAACAGTTTCACAGGGCTGCCAATTTATATTTCCTGTTCCTCGTGGTCCTGAACT
  GGGTGCCTTTGGTAGAAGCCTTCCAAAAGGAAATCACCATGCTGCCTCTGGTGGTGGTCCTCACAATTAT
  TGCAATTAAAGATGGCTTGGAAGACTACCGGAAATACAAAATCGACAAGCAGATCAACAACTTAATAACC
  AAAGTTTACAGTAGGAAAGAAAAAAAGTACATTGACTGTTGCTGGAAAAATGTCACCGTCGGGGACTTCA
  TCCGCCTGTCCTGCAATGAGATCATCCCTGCAGACATGGTCTTACTCTTTTCCACAGACCCAGATGGCAT
  CTGCCACATTGAGACTTCCGGCCTTGATGGAGAGAGCAATTTAAAGCAGAGGCAGGTGGTGCGTGGCTAC
  ACAGAGCAGGACTCTGAGGTTGATCCGGAGAAGTTCTCCAGCAGGATAGAATGTGAAAGTCCAAACAATG
  ACCTCAGCCGATTCCGAGGCTTCCTGGAACATGCCAATAAAGAACGTGTGGGCCTCAGCAAAGAGAATTT
  ATTGCTCCGCGGGTGCACCATCAGAAACACAGAGGCTGTGGTGGGCATTGTGGTCTATGCAGGTCATGAA
  ACCAAAGCAATGCTGAACAACAGTGGGCCACGGTATAAGCGCAGTAAGTTAGAGAGAAGAGCAAACACCG
  ACGTCCTCTGGTGTGTCCTGCTTCTGATTGTCATGTGCTTAACTGGTGCACTGGGTCACGGCATATGGCT
  GAGCAGGTATGAGAACATGCTCTTTTTTAACATCCCTGAGCCGGATGGACGTGTCATCTCACCCGTGCTG
  ACTGGATTCTATGTGTTCTGGACCATGATCATCTTGCTGCAGGTCCTGATCCCCATTTCTCTCTATGTGT
  CCATTGAGATCGTGAAGCTTGGGCAGATCTATTTCATCCAGAGCGATGTAGATTTCTACAACGAGAAAAT
  GGATTCTACCATTCAGTGCCGAGCCCTGAACATCACTGAGGACCTTGGGCAGATTCAATACCTCTTTTCT
  GATAAGACAGGAACCCTCACTGAGAATAAGATGGTGTTTCGGAGGTGCAGTGTAGCAGGGTTTGACTACT
  GTCATGAAGAAAACGCCAAGAGGCTCGAGTCCTATCAGGAAGCTGTCTCTGAAGAGGAGGAATGCACAGA
  CACTCTCGGCGGCTCCCTCAGCAACATGGCGAGACCCAGAGCCCAGGGCTGCAGGACAGTTCCCAGTGGG
  CCTCTGGGAAAGCCCTCGGCTCAGCTCTCCGGGAGCACCTCTGCTGTAGGAAATGGAGAAGGATCTGGGG
  AAGTGCCTCATTCCAGACAGGCCGCCTTCAGTAGTCCCATGGAAACAGACGTGGTACCAGATACCAGACT
  TTTAGACAAATTTAGCCAGCTTACCCCTCAGCTGCTCACTGGACTGGATGGCACCGCTCAGAGTTCACCG
  CTGGAGACTTTGTACATCATGGACTTCTTTATTGCACTGGCAATTTGCAACACGGTGGTGGTTTCTGCAC
  CGAACCAACCTCGGCAAAAGATTGGACTCTCCTCACTGGGTGGAATGCCCATCAAGTCCTTGGAAGAGAT
  TAAAAACATCTTCCAGAAATTGTCCGTCCGGAGATCAAGTTCACCGTCCCTTGCCAGTGGGAAGGATTCA
  TCCTCTGGGACTCCCTGTGCCTTTGTGAGCAGAATCTCTTTCTTTAGTCGACCAAAACTGTCACCTCCTA
  TGGAGGACGAGTCTTCCCAAATGGATGAAATCCCCCAGGCCAGTAACTCAGCTTGCTGTACAGAAACAGA
  GGCACAAAACAGGGCCGTAGGACTCAGTGTCAGTTCCGCTGAAGCCCTAAGTGGACCACCGCCCTCGGCT
  TCCAACCTGTGCTATGAGGCGGAGAGTCCAGATGAAGCAGCTTTGGTGTATGCCGCCAGAGCTTACCGTT
  GCACTTTACAGTCTCGGACCCCAGAGCAGGTCATGGTGGACTTTGCTGCTTTGGGCTCATTAACATTTCA
  ACTCCTACACATTCTGCCCTTTGACTCTGTAAGGAAAAGAATGTCGGTCGTGGTCAGGCATCCTCTTTCC
  AAACAAGTCGTGGTGTATACAAAAGGCGCTGATTCTGTTATCATGGAGCTGCTGTCTGTGGCTGCCTCAG
  ATGGAACAAATCCAGAACAACAGATGATAATAAGGGAGAGAACGCAGAGGCACCTGGACGAGTATGCCAA
  ACGAGGGCTGCGCACTCTATGTGTAGCAAAGAAGGTCATGAGTGACACGGAATATGCAGAGTGGCTGAGG
  AATCACTTTCTAGCTGAAACCAGCATTGACAACAGGGAGGAACTGCTAGTTGAATCTGCCATGAGACTAG
  AAAACAAACTCACGTTACTTGGTGCTACTGGCATTGAGGATCGTCTGCAGGAGGGGGTCCCTGAGTCTAT
  AGAAGCCCTTCACCAAGCTGGCATCAAGATCTGGATGCTGACAGGGGACAAGCAGGAGACAGCTGTCAAC
  ATAGCTTATGCATGCAAACTCCTGGAGCCAGATGACAAGCTCTTCATCCTCAATACACAGAGTCAGGATG
  CCTGTGGGATGCTGATGAGTGCAATTTTGGAAGAACTTCAGAAGAGAGCTCAAGTGTCTCCGGAGCTGGC
  ATCATCAAGAAAGAACTTTCCTCAGCCCTCTGACGCTCAGGGCCAGGGACGTGCGGGACTTGTTATCACT
  GGGAAGACCCTGGAGTTTGCCCTGCAGGAGAGTCTGCAAAGGCAGTTCCTTGAGCTGACCGCGTGGTGCC
  AAGCTGTGATCTGCTGCCGAGCCACCCCCCTTCAAAAAAGTGAGGTGGTGAAATTGGTTCGAAACCATCA
  CCATGTGCTGACCCTACCCATTGGTGACGGTGCCAATGATGTTAGCATGATACAAGTGGCCGACATTGGG
  ATCGGTGTCTCAGGTCAAGAAGGCATGCAGGCTGTGATGGCCAGTGACTTTGCCATCTCCCAGTTCAGAC
  ATCTCAGCAAACTTCTCCTTGTGCACGGGCACTGGTGTTACACCCGGCTCTCCAACATGATTCTCTATTT
  TTTCTACAAGAATGTGGCCTATGTGAATCTCCTTTTCTGGTACCAGTTCTTTTGTGGGTTTTCAGGAACA
  TCTATGACAGACTACTGGGTGCTGATCTTCTTCAACCTCCTCTTCACATCTGTACCCCCCATCATTTATG
  GCGTTTTGGAGAAAGATGTTTCGGCAGAGACCCTCCTGCAGCTGCCTGAACTTTACCGGAGCGGTCAGCG
  ATCAGAGGAGTACTTGCCCCTCACCTTCTGGATCACCTTGTTGGATGCCTTTTATCAAAGCCTGGTCTGC
  TTCTTCGTGCCTTACTTTACCTACCAGGGCTCTGACATTGACATCTTCACCTTTGGGAATCCCCTGAACA
  CGGCGGCTCTGTTCATCATTCTCCTCCACCTGGTGATTGAAAGCAAGAGTTTGACTTGGATCCACATGCT
  GGTCACGGTTGGGAGCATCTTGTCCTACTTTTTCTTTGCCTTGGCTTTTGGAGCCTTATGTGTCACTTGC
  AACCCACCCTCCAACCCCTACGGGATCATGCGGAAGCACATGCTGGACCCTGTGTTCTACTTGGTTTGTG
  TACTTACAACCTTCGTCGCGCTCCTGCCGAGGTTTCTTTACCGAGTTCTTCAGGGATCCGTGTTTCCATC
  TCCAGTTCTCAGAGCCAAGTACTTTGACAGACTACCTCCAGAGGAGAGAGCTGAAGCTCTCAAGAGGTGG
  AGAGGGACAGCAAAGGTCAATCACGTGGCATCTAAGCATGCCAGCCAATCAGCTGCTATGTCAGGAAGAC
  CAACACCTGGGTCTTCTGCTGTCCTTGCAATGAAGTCAGCAACAGTGTCTACTGTTGAGCAGAGCACACG
  TGAGACTGCGCTAGACCGTGGCTGCTCTGAACCTGGGGCCTCCAAGATGACTGGATCCTCAGCAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223765 representing NM_153389
  Red=Cloning site Green=Tags(s)
  
  MTNGWLGSVTLPALWISAPFSFGTLGSEVTPCWDERPGRDRSPEPAGSPCVLGMTELLQWARHHWRRLSH
  GRAQGEDERPYNYASLLACGGKSSRTPRPAGKHRVVIPHLQCFKDEYERFSGTYVNNRIRTTKYTLLNFV
  PRNLFEQFHRAANLYFLFLVVLNWVPLVEAFQKEITMLPLVVVLTIIAIKDGLEDYRKYKIDKQINNLIT
  KVYSRKEKKYIDCCWKNVTVGDFIRLSCNEIIPADMVLLFSTDPDGICHIETSGLDGESNLKQRQVVRGY
  TEQDSEVDPEKFSSRIECESPNNDLSRFRGFLEHANKERVGLSKENLLLRGCTIRNTEAVVGIVVYAGHE
  TKAMLNNSGPRYKRSKLERRANTDVLWCVLLLIVMCLTGALGHGIWLSRYENMLFFNIPEPDGRVISPVL
  TGFYVFWTMIILLQVLIPISLYVSIEIVKLGQIYFIQSDVDFYNEKMDSTIQCRALNITEDLGQIQYLFS
  DKTGTLTENKMVFRRCSVAGFDYCHEENAKRLESYQEAVSEEEECTDTLGGSLSNMARPRAQGCRTVPSG
  PLGKPSAQLSGSTSAVGNGEGSGEVPHSRQAAFSSPMETDVVPDTRLLDKFSQLTPQLLTGLDGTAQSSP
  LETLYIMDFFIALAICNTVVVSAPNQPRQKIGLSSLGGMPIKSLEEIKNIFQKLSVRRSSSPSLASGKDS
  SSGTPCAFVSRISFFSRPKLSPPMEDESSQMDEIPQASNSACCTETEAQNRAVGLSVSSAEALSGPPPSA
  SNLCYEAESPDEAALVYAARAYRCTLQSRTPEQVMVDFAALGSLTFQLLHILPFDSVRKRMSVVVRHPLS
  KQVVVYTKGADSVIMELLSVAASDGTNPEQQMIIRERTQRHLDEYAKRGLRTLCVAKKVMSDTEYAEWLR
  NHFLAETSIDNREELLVESAMRLENKLTLLGATGIEDRLQEGVPESIEALHQAGIKIWMLTGDKQETAVN
  IAYACKLLEPDDKLFILNTQSQDACGMLMSAILEELQKRAQVSPELASSRKNFPQPSDAQGQGRAGLVIT
  GKTLEFALQESLQRQFLELTAWCQAVICCRATPLQKSEVVKLVRNHHHVLTLPIGDGANDVSMIQVADIG
  IGVSGQEGMQAVMASDFAISQFRHLSKLLLVHGHWCYTRLSNMILYFFYKNVAYVNLLFWYQFFCGFSGT
  SMTDYWVLIFFNLLFTSVPPIIYGVLEKDVSAETLLQLPELYRSGQRSEEYLPLTFWITLLDAFYQSLVC
  FFVPYFTYQGSDIDIFTFGNPLNTAALFIILLHLVIESKSLTWIHMLVTVGSILSYFFFALAFGALCVTC
  NPPSNPYGIMRKHMLDPVFYLVCVLTTFVALLPRFLYRVLQGSVFPSPVLRAKYFDRLPPEERAEALKRW
  RGTAKVNHVASKHASQSAAMSGRPTPGSSAVLAMKSATVSTVEQSTRETALDRGCSEPGASKMTGSSAS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153389
• ORF Size: 4407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153389.3, NP_700438.3
• RefSeq Size: 6021 bp
• RefSeq ORF: 4410 bp
• Locus ID: 231287
• UniProt ID: Q8K2X1
• Cytogenetics: 5 C3.2
• Gene Summary: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (By similarity).[UniProtKB/Swiss-Prot Function]