Atp10d (NM_153389) Mouse Untagged Clone

CAT#: MC224523

Atp10d (untagged) - Mouse ATPase, class V, type 10D (Atp10d), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp10d
• Synonyms: 9830145H18Rik; ATPVD; D5Buc24e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224523 representing NM_153389
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAATGGCTGGCTTGGAAGCGTAACTCTCCCCGCCCTTTGGATCTCTGCTCCGTTCTCTTTTGGCA
  CTTTGGGATCCGAGGTAACACCATGCTGGGATGAGCGGCCCGGGAGGGACAGATCGCCTGAGCCAGCCGG
  GTCTCCCTGCGTCTTGGGCATGACTGAGCTGCTGCAGTGGGCCAGACATCACTGGCGTCGGCTGAGCCAT
  GGGAGAGCCCAGGGTGAAGATGAGAGGCCATACAACTACGCCTCCCTGCTGGCCTGTGGGGGCAAGTCCT
  CGAGGACCCCCAGACCTGCAGGAAAGCACCGCGTCGTTATTCCCCACCTTCAGTGCTTCAAAGATGAGTA
  CGAAAGGTTTTCTGGAACCTACGTGAATAACCGGATACGGACGACCAAGTACACACTCCTGAACTTTGTG
  CCAAGAAACTTATTTGAACAGTTTCACAGGGCTGCCAATTTATATTTCCTGTTCCTCGTGGTCCTGAACT
  GGGTGCCTTTGGTAGAAGCCTTCCAAAAGGAAATCACCATGCTGCCTCTGGTGGTGGTCCTCACAATTAT
  TGCAATTAAAGATGGCTTGGAAGACTACCGGAAATACAAAATCGACAAGCAGATCAACAACTTAATAACC
  AAAGTTTACAGTAGGAAAGAAAAAAAGTACATTGACTGTTGCTGGAAAAATGTCACCGTCGGGGACTTCA
  TCCGCCTGTCCTGCAATGAGATCATCCCTGCAGACATGGTCTTACTCTTTTCCACAGACCCAGATGGCAT
  CTGCCACATTGAGACTTCCGGCCTTGATGGAGAGAGCAATTTAAAGCAGAGGCAGGTGGTGCGTGGCTAC
  ACAGAGCAGGACTCTGAGGTTGATCCGGAGAAGTTCTCCAGCAGGATAGAATGTGAAAGTCCAAACAATG
  ACCTCAGCCGATTCCGAGGCTTCCTGGAACATGCCAATAAAGAACGTGTGGGCCTCAGCAAAGAGAATTT
  ATTGCTCCGCGGGTGCACCATCAGAAACACAGAGGCTGTGGTGGGCATTGTGGTCTATGCAGGTCATGAA
  ACCAAAGCAATGCTGAACAACAGTGGGCCACGGTATAAGCGCAGTAAGTTAGAGAGAAGAGCAAACACCG
  ACGTCCTCTGGTGTGTCCTGCTTCTGATTGTCATGTGCTTAACTGGTGCACTGGGTCACGGCATATGGCT
  GAGCAGGTATGAGAACATGCTCTTTTTTAACATCCCTGAGCCGGATGGACGTGTCATCTCACCCGTGCTG
  ACTGGATTCTATGTGTTCTGGACCATGATCATCTTGCTGCAGGTCCTGATCCCCATTTCTCTCTATGTGT
  CCATTGAGATCGTGAAGCTTGGGCAGATCTATTTCATCCAGAGCGATGTAGATTTCTACAACGAGAAAAT
  GGATTCTACCATTCAGTGCCGAGCCCTGAACATCACTGAGGACCTTGGGCAGATTCAATACCTCTTTTCT
  GATAAGACAGGAACCCTCACTGAGAATAAGATGGTGTTTCGGAGGTGCAGTGTAGCAGGGTTTGACTACT
  GTCATGAAGAAAACGCCAAGAGGCTCGAGTCCTATCAGGAAGCTGTCTCTGAAGAGGAGGAATGCACAGA
  CACTCTCGGCGGCTCCCTCAGCAACATGGCGAGACCCAGAGCCCAGGGCTGCAGGACAGTTCCCAGTGGG
  CCTCTGGGAAAGCCCTCGGCTCAGCTCTCCGGGAGCACCTCTGCTGTAGGAAATGGAGAAGGATCTGGGG
  AAGTGCCTCATTCCAGACAGGCCGCCTTCAGTAGTCCCATGGAAACAGACGTGGTACCAGATACCAGACT
  TTTAGACAAATTTAGCCAGCTTACCCCTCAGCTGCTCACTGGACTGGATGGCACCGCTCAGAGTTCACCG
  CTGGAGACTTTGTACATCATGGACTTCTTTATTGCACTGGCAATTTGCAACACGGTGGTGGTTTCTGCAC
  CGAACCAACCTCGGCAAAAGATTGGACTCTCCTCACTGGGTGGAATGCCCATCAAGTCCTTGGAAGAGAT
  TAAAAACATCTTCCAGAAATTGTCCGTCCGGAGATCAAGTTCACCGTCCCTTGCCAGTGGGAAGGATTCA
  TCCTCTGGGACTCCCTGTGCCTTTGTGAGCAGAATCTCTTTCTTTAGTCGACCAAAACTGTCACCTCCTA
  TGGAGGACGAGTCTTCCCAAATGGATGAAATCCCCCAGGCCAGTAACTCAGCTTGCTGTACAGAAACAGA
  GGCACAAAACAGGGCCGTAGGACTCAGTGTCAGTTCCGCTGAAGCCCTAAGTGGACCACCGCCCTCGGCT
  TCCAACCTGTGCTATGAGGCGGAGAGTCCAGATGAAGCAGCTTTGGTGTATGCCGCCAGAGCTTACCGTT
  GCACTTTACAGTCTCGGACCCCAGAGCAGGTCATGGTGGACTTTGCTGCTTTGGGCTCATTAACATTTCA
  ACTCCTACACATTCTGCCCTTTGACTCTGTAAGGAAAAGAATGTCGGTCGTGGTCAGGCATCCTCTTTCC
  AAACAAGTCGTGGTGTATACAAAAGGCGCTGATTCTGTTATCATGGAGCTGCTGTCTGTGGCTGCCTCAG
  ATGGAACAAATCCAGAACAACAGATGATAATAAGGGAGAGAACGCAGAGGCACCTGGACGAGTATGCCAA
  ACGAGGGCTGCGCACTCTATGTGTAGCAAAGAAGGTCATGAGTGACACGGAATATGCAGAGTGGCTGAGG
  AATCACTTTCTAGCTGAAACCAGCATTGACAACAGGGAGGAACTGCTAGTTGAATCTGCCATGAGACTAG
  AAAACAAACTCACGTTACTTGGTGCTACTGGCATTGAGGATCGTCTGCAGGAGGGGGTCCCTGAGTCTAT
  AGAAGCCCTTCACCAAGCTGGCATCAAGATCTGGATGCTGACAGGGGACAAGCAGGAGACAGCTGTCAAC
  ATAGCTTATGCATGCAAACTCCTGGAGCCAGATGACAAGCTCTTCATCCTCAATACACAGAGTCAGGATG
  CCTGTGGGATGCTGATGAGTGCAATTTTGGAAGAACTTCAGAAGAGAGCTCAAGTGTCTCCGGAGCTGGC
  ATCATCAAGAAAGAACTTTCCTCAGCCCTCTGACGCTCAGGGCCAGGGACGTGCGGGACTTGTTATCACT
  GGGAAGACCCTGGAGTTTGCCCTGCAGGAGAGTCTGCAAAGGCAGTTCCTTGAGCTGACCGCGTGGTGCC
  AAGCTGTGATCTGCTGCCGAGCCACCCCCCTTCAAAAAAGTGAGGTGGTGAAATTGGTTCGAAACCATCA
  CCATGTGCTGACCCTACCCATTGGTGACGGTGCCAATGATGTTAGCATGATACAAGTGGCCGACATTGGG
  ATCGGTGTCTCAGGTCAAGAAGGCATGCAGGCTGTGATGGCCAGTGACTTTGCCATCTCCCAGTTCAGAC
  ATCTCAGCAAACTTCTCCTTGTGCACGGGCACTGGTGTTACACCCGGCTCTCCAACATGATTCTCTATTT
  TTTCTACAAGAATGTGGCCTATGTGAATCTCCTTTTCTGGTACCAGTTCTTTTGTGGGTTTTCAGGAACA
  TCTATGACAGACTACTGGGTGCTGATCTTCTTCAACCTCCTCTTCACATCTGTACCCCCCATCATTTATG
  GCGTTTTGGAGAAAGATGTTTCGGCAGAGACCCTCCTGCAGCTGCCTGAACTTTACCGGAGCGGTCAGCG
  ATCAGAGGAGTACTTGCCCCTCACCTTCTGGATCACCTTGTTGGATGCCTTTTATCAAAGCCTGGTCTGC
  TTCTTCGTGCCTTACTTTACCTACCAGGGCTCTGACATTGACATCTTCACCTTTGGGAATCCCCTGAACA
  CGGCGGCTCTGTTCATCATTCTCCTCCACCTGGTGATTGAAAGCAAGAGTTTGACTTGGATCCACATGCT
  GGTCACGGTTGGGAGCATCTTGTCCTACTTTTTCTTTGCCTTGGCTTTTGGAGCCTTATGTGTCACTTGC
  AACCCACCCTCCAACCCCTACGGGATCATGCGGAAGCACATGCTGGACCCTGTGTTCTACTTGGTTTGTG
  TACTTACAACCTTCGTCGCGCTCCTGCCGAGGTTTCTTTACCGAGTTCTTCAGGGATCCGTGTTTCCATC
  TCCAGTTCTCAGAGCCAAGTACTTTGACAGACTACCTCCAGAGGAGAGAGCTGAAGCTCTCAAGAGGTGG
  AGAGGGACAGCAAAGGTCAATCACGTGGCATCTAAGCATGCCAGCCAATCAGCTGCTATGTCAGGAAGAC
  CAACACCTGGGTCTTCTGCTGTCCTTGCAATGAAGTCAGCAACAGTGTCTACTGTTGAGCAGAGCACACG
  TGAGACTGCGCTAGACCGTGGCTGCTCTGAACCTGGGGCCTCCAAGATGACTGGATCCTCAGCAAGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153389
• Insert Size: 4410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153389.3, NP_700438.3
• RefSeq Size: 6021 bp
• RefSeq ORF: 4410 bp
• Locus ID: 231287
• UniProt ID: Q8K2X1
• Cytogenetics: 5 C3.2
• Gene Summary: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1, coding) represents the protein-coding transcript. Sequence Note: This RefSeq record was created to represent the transcript and the full-length, functional protein that is expressed in several strains. It does not contain a nonsense mutation that truncates the CDS in the strain of the reference genome, C57BL/6J.