Zeb1 (NM_011546) Mouse Tagged ORF Clone

CAT#: MG223095

Zeb1 (tGFP-tagged) - Mouse zinc finger E-box binding homeobox 1 (Zeb1), (10ug)

Product Images

Circular map for MG223095

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Zeb1
• Synonyms: 3110032K11Rik; AREB6; BZP; MEB1; Nil2; TCF-8; Tcf8; Tcf18; Tw; ZEB; Zfhep; Zfhx1a; Zfx1a; Zfx1ha
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223095 representing NM_011546
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATGGCCCCAGGTGTAAGCGCAGAAAGCAGGCGAACCCGCGGCGCAATAACGTTACAAATTATA
  ATACTGTGGTAGAAGCAAATTCAGATTCCGATGATGAAGACAAACTCCATATTGTGGAAGAAGAAAGTAT
  TACAGATGCAGCTGACTGTGAAGGTGGCATGCCAGATGATGAACTGCCAGCAGACCAGACAGTATTACCA
  GGAGGCAGTGACAGGGGGGGCGGTGCCAAGAACTGCTGGCAAGACAACGTGAAAGACAACGAGTGTGACT
  CAGATGCAGAAAATGAGCAAAACCATGATCCGAATGTGGAAGAATTTCTGCAGCAACAAGACACCGCCGT
  CATTTATCCTGAGGCGCCCGAGGAAGACCAGCGGCAGGGCACACCAGAAGCCAGCAGTCATGATGAAAAC
  GGAACACCAGATGCATTTTCCCAGTTGCTCACCTGCCCGTATTGTGATAGAGGCTACAAGCGCTTTACCT
  CTTTGAAAGAACACATTAAGTACCGCCATGAGAAGAACGAGGACAACTTCAGCTGCTCCCTGTGCAGTTA
  CACCTTTGCATACAGAACCCAGCTTGAACGTCATATGACATCACATAAGTCAGGAAGAGAGCAAAGACAT
  GTGACACAGTCTGGGGGAAACCGCAAGTTCAAGTGCACTGAATGCGGGAAGGCCTTCAAGTACAAACACC
  ACCTGAAAGAGCACTTACGGATTCACAGTGGAGAGAAGCCATACGAATGCCCGAACTGCAAGAAACGGTT
  TTCCCATTCTGGCTCCTATAGCTCACATATAAGCAGTAAGAAGTGTATTAGCTTGATGCCTGTGAATGGC
  AGGCCTAGATCAGGACTCAAGACATCTCAGTGTTCCTCGCCATCTCTTTCGACATCACCAGGCAGTCCCA
  CACGCCCACAGATACGACAGAAGATAGAGAATAAACCCCTTCAAGAACCGCTTTCTGTAAACCAAATCAA
  AACTGAACCTGTGGATTATGAGTTCAAACCCATAGTGGTTGCTTCAGGAATCAACTGTTCAACCCCTTTA
  CAAAATGGGGTTTTTAGCAGTGGTGGCCAATTGCAGGCAACCAGTTCTCCTCAGGGTGTGGTGCAAGCCG
  TTGTTCTGCCAACAGTTGGTTTGGTATCTCCCATAAGTATCAACTTAAGTGACATTCAGAATGTACTTAA
  AGTGGCTGTAGATGGTAACGTAATACGACAAGTCTTGGAGACTAATCAAGCCAGTCTTGCATCCAAAGAG
  CAAGAAGCAGTGAGTGCTTCGCCCATCCAGCAGGGTGGCCATTCTGTCATTTCTGCCATCAGTCTTCCTT
  TAGTTGATCAGGATGGAACAACCAAAATCATCATCAACTACAGTCTTGAGCAGCCCAGTCAACTTCAGGT
  TGTTCCCCAGAATTTAAAGAAAGAAATCCCAGCCCCTACAAACAGCTGCAAAAGTGAGAAGTTACCAGAA
  GACCTTACTGTCAAATCAGAAACGGACAAAAGCTTTGAGGGGGCCAGGGATGATAGCACTTGCCTTCTGT
  GTGAGGACTGCCCAGGGGACCTCAATGCACTTCCAGAACTAAAGCACTATGACCCAGAGTGCCCTGCTCA
  GCCTCCACCCCCTGCCCCAGCCACCGAGAAGCCAGAGTCCTCTGCTTCATCAGCTGGAAACGGAGATTTG
  TCTCCCAGTCAGCCACCTTTAAAGAACCTTCTGTCACTCTTGAAAGCCTACTATGCTCTGAACGCGCAGC
  CAAGCACAGAAGAGCTCTCAAAGATCGCCGATTCTGTGAACCTACCGCTGGATGGAGTTAAAAAGTGGTT
  TGAAAAGATGCAAGCTGGACAGATTCCAGGACAGTCTCCTGACCCCCCTTCTCCTGGAACCGGGTCAGTA
  AACATACCTACAAAAACCGATGAGCAGCCTCAACCTGCGGATGGAAATGAGCCCCAGGAAGACAGCACAC
  GCGGACAGAGTCCTGTCAAGATAAGGAGCTCTCCGGTTTTACCTGTAGGATCAGCCATGAACGGTTCCAG
  AAGCTGCACATCATCCCCATCCCCTCTAAACCTTTGCTCAGCCAGGAACCCGCAGGGTTACTCTTGTGTG
  GCAGAGGGTGCCCAGGAGGAGCCCCAAGTAGAACCTCTTGATCTCTCACTACCAAAGCAACAGGGAGAGT
  TACTGGAAAGGTCGACAGTCAGTAGCGTTTACCAGAACAGTGTTTATTCTGTCCAGGAAGAACCCTTGAA
  CTTGTCTTGTGCAAAAAAGGAACCACAAAAGGACAGCTGTGTTACAGACTCAGAACCAGTTGTAAATGTA
  GTCCCACCAAGTGCCAACCCCATAAACATTGCTATTCCTACAGTCACTGCCCAGTTACCCACAATCGTGG
  CCATTGCTGACCAGAACAGTGTTCCATGTTTAAGAGCACTGGCCGCCAACAAGCAGACTATTCTGATTCC
  CCAAGTGGCATATACATATTCAGCTACTGTGAGCCCTGCCGTGCAGGAGCCGCCAGTGAAGGTGATCCAG
  CCAAACGGAAACCAGGATGAAAGACAAGACACTAGCTCAGAAGGAGTCTCCACTGTGGAGGACCAGAATG
  ACTCTGACTCCACGCCACCCAAAAAGAAAACTCGGAAGACAGAGAATGGAATGTATGCATGTGACCTGTG
  TGACAAGATATTTCAGAAGAGCAGCTCACTGTTGAGACACAAATATGAGCACACAGGTAAGAGGCCTCAC
  GAGTGTGGAATCTGTAGAAAGGCATTTAAACACAAGCATCATTTGATTGAGCACATGCGGCTGCACTCTG
  GGGAAAAGCCCTATCAATGTGACAAGTGTGGCAAGCGCTTCTCACACTCCGGCTCCTACTCTCAACACAT
  GAATCACCGCTACTCCTACTGCAAGAGAGGAGCTGAAGACAGAGATGCTATGGAGCAGGAAGACGCTGGG
  CCCGAAGTCCTGCCGGAAGTCCTGGCGACTGAGCATGTTGGTGCCCGGGCGTCTCCCTCACAGGCTGACT
  CGGACGAGAGAGAAAGTCTGACAAGGGAAGAAGATGAAGACAGTGAAAAGGAGGAGGAGGAGGAAGATAA
  AGAGATGGAAGAATTACAGGAAGGAAAGGAATGTGAGAACCCACAGGGGGAGGAGGAGGAGGAGGAGGAG
  GAGGAAGAGGAGGAAGAAGAGGAAGAAGAGGAGGAAGTAGAGGCGGATGAAGCCGAGCATGAGGCAGCAG
  CCAAGACTGATGGTACAGTGGAGGGTGGAGCTGCACAGCAGGCAGGCAGCTTAGAGCAGAAGGCCAGCGA
  GAGCGAGATGGAGAGCGAGAGTGAGAGTGAGCAGCTGTCTGAAGAGAAGACAAATGAAGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223095 representing NM_011546
  Red=Cloning site Green=Tags(s)
  
  MADGPRCKRRKQANPRRNNVTNYNTVVEANSDSDDEDKLHIVEEESITDAADCEGGMPDDELPADQTVLP
  GGSDRGGGAKNCWQDNVKDNECDSDAENEQNHDPNVEEFLQQQDTAVIYPEAPEEDQRQGTPEASSHDEN
  GTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSLCSYTFAYRTQLERHMTSHKSGREQRH
  VTQSGGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCISLMPVNG
  RPRSGLKTSQCSSPSLSTSPGSPTRPQIRQKIENKPLQEPLSVNQIKTEPVDYEFKPIVVASGINCSTPL
  QNGVFSSGGQLQATSSPQGVVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLETNQASLASKE
  QEAVSASPIQQGGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLQVVPQNLKKEIPAPTNSCKSEKLPE
  DLTVKSETDKSFEGARDDSTCLLCEDCPGDLNALPELKHYDPECPAQPPPPAPATEKPESSASSAGNGDL
  SPSQPPLKNLLSLLKAYYALNAQPSTEELSKIADSVNLPLDGVKKWFEKMQAGQIPGQSPDPPSPGTGSV
  NIPTKTDEQPQPADGNEPQEDSTRGQSPVKIRSSPVLPVGSAMNGSRSCTSSPSPLNLCSARNPQGYSCV
  AEGAQEEPQVEPLDLSLPKQQGELLERSTVSSVYQNSVYSVQEEPLNLSCAKKEPQKDSCVTDSEPVVNV
  VPPSANPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSATVSPAVQEPPVKVIQ
  PNGNQDERQDTSSEGVSTVEDQNDSDSTPPKKKTRKTENGMYACDLCDKIFQKSSSLLRHKYEHTGKRPH
  ECGICRKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKRGAEDRDAMEQEDAG
  PEVLPEVLATEHVGARASPSQADSDERESLTREEDEDSEKEEEEEDKEMEELQEGKECENPQGEEEEEEE
  EEEEEEEEEEEEVEADEAEHEAAAKTDGTVEGGAAQQAGSLEQKASESEMESESESEQLSEEKTNEA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011546
• ORF Size: 3351 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011546.2, NP_035676.1
• RefSeq Size: 5218 bp
• RefSeq ORF: 3354 bp
• Locus ID: 21417
• UniProt ID: Q64318
• Cytogenetics: 18 A1
• Gene Summary: Acts as a transcriptional repressor. Binds to E-box sequences in the immunoglobulin heavy chain enhancer as well as in the regulatory regions of many other tissue-specific genes. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1 (By similarity). Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.[UniProtKB/Swiss-Prot Function]