Patj (NM_001005784) Mouse Tagged ORF Clone

CAT#: MG222656

Inadl (tGFP-tagged) - Mouse InaD-like (Drosophila) (Inadl) transcript variant 2, (10ug)

Product Images

Circular map for MG222656

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Patj
• Synonyms: C; Cipp; I; Inadl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222656 representing NM_001005784
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAAAACCCTGCTGCAGAGAAGATGCAGGTCCTGCAGGTCCTGGATCGCCTTCGAGGGAAGCTGC
  AGGAGAAGGGAGACACGACGCAGAACGAGAAGCTGTCTGCGTTCTACGAGACGCTGAAGAGCCCTCTCTT
  CAACCAGATCCTTACACTGCAGCAGTCCATCAAGCAGCTGAAGGGACAGCTTAGCCACATCCCCTCAGAT
  TGCTCAGCCAACTTTGACTTTTCTCGCAAGGGCTTGCTAGTGTTCACGGATGGTTCCATTACAAACGGGA
  ATGCCCAAAGGCCTTGTAGTAATGTGACTGCATCTGAACTGTTGCCCTGGACTCAAAAGTCAGCAAGTGA
  AGACTTTAACTCAGTCATTCAGCAGATGGCTCAGGGCCGGCACGTTGAGTATATAGATATTGAACGTCCT
  TCAACTGGAGGCCTTGGATTCAGTGTGGTGGCTCTGAGAAGCCAAAGCCTGGGATTAATTGATATCTTTG
  TGAAGGAAGTCCATCCCGGGAGTGTAGCAGACAGGGATCACAGATTGAAGGAAAATGACCAGATTTTGGC
  CATTAATGACACCCCATTGGATCAGAATATTTCCCATCAGCAGGCGATTGCATTACTACAACAAGCCACT
  GGATCTCTGCGGCTGGTTGTGGCCAGGGAAGTGGGCCACACACAGGGCCGAGCATCCACCAGCTCAGCTG
  ATACAACTCTGCCAGAAACGGTGTGTTGGGGCCACACGGAAGAGGTTGAGCTCATTAACGATGGCTCTGG
  ATTAGGTTTTGGGATAGTTGGAGGGAAATCCAGCGGCGTGGTCGTGAGGACTATTGTTCCTGGAGGACTG
  GCAGACCGAGATGGAAGACTGCAGACAGGGGATCACATCTTGAAGATTGGTGGTACAAATGTTCAAGGGA
  TGACCAGTGAACAAGTTGCTCAAGTGCTAAGGAACTGTGGGAATTCAGTGAGGATGCTGGTGGCCAGAGA
  CCCTGTTGGTGAAATTGCAGTAACACCTCCTACCCCTGTATCCTTACCTGTTGCCCTACCTGCTGTAGCC
  ACAAGGACCCTTGATTCTGACAGGTCTCCTTTTGAAACTTACAGTGTTGAACTTGTTAAGAAAGATGGGC
  AGAGTCTTGGGATTAGAATTGTTGGCTATGTTGGAACAGCTCATCCAGGGGAAGCTTCCGGGATTTATGT
  GAAAAGTATAATTCCAGGCAGCGCTGCATACCACAATGGCCAAATTCAAGTGAATGATAAAATCGTGGCT
  GTGGATGGTGTGAATATTCAAGGTTTTGCTAATCAGGATGTTGTCGAAGTATTGCGGAATGCTGGGCAGG
  TGGTACACCTGACCCTTGTTCGAAGGAAAACATCTTTATCTGCTTCTCCGTTTGAGCACTCTTCAAGCAG
  AGAAACTGTTGCAGAACCACCTAAAGTACCAGAACGAGCAGGATCCCCGAAACCAGAAGCTAATTTGAGT
  GTGGAAGCAGAGGAAATTGGAGAAAGACTGGATAATCTAAAAAACAACACTGTACAAGCCTTAGAAAAAC
  CAGATGTATATCCAGAGAAAGTTCCAGGCTCTCCAGAAAATGAGCTGAAATCCAGATGGGAAAACCTACT
  GGGCCCAGATTATGAAGTAATGGTAGCCACTTTGGATGCTCAGATTGCAGACGATGAGGAGCTACAGAAA
  TACTCGAAGCTGCTGCCTATCCACACGCTCAGGCTTGGGATGGAAGTCGACTCCTTCGATGGGCACCACT
  ATATCTCCTCAGTTGCTCCTGGGGGTCCTGTGGATACCCTGAACCTCCTTCAGCCAGAGGATGAACTTCT
  TGAGGTCAACGGCATGCAGCTTTATGGGAAGTCTCGCCGAGAAGCAGTCTCCTTCCTTAAAGAAGTGCCC
  CCACCATTTACCTTGGTCTGCTGCCGCCGGCTGTTTGATGATGAGGCCTCAGTTGATGAGCCAAGAACCA
  TGGAGCCTGCTCTTCTTGAGGCCGAGGTTGACCACAGTGTAGATGTCAACATTGAAGATGATGATGATGG
  AGAATTAGCCCTGTGGTCTCCTGAAGTCAAGACTGTGGAGCTGGTGAAAGACTGCAAAGGCTTAGGATTC
  AGTATTTTGGATTATCAGGACCCCCTGGATCCCACGCGGTCAGTGATTGTGATTCGCTCCTTGGTGGCAG
  ATGGTGTAGCGGAAAGAAGTGGAGAACTTTTACCTGGAGATCGCCTAGTCTCCGTCAATGAGTTCTCCTT
  GGACAATGCCACTCTTGCTGAGGCTGTGGAAGTGTTGAAGGCTGTACCCCCAGGTGTGGTGCATCTTGGC
  ATCTGTAAGCCTTTGGTGGAAGATGAGAAGGAAGAGCGTTTTAGTTTACATTCAAACAACAATGGTGACA
  GCAGCGAGCCTGCAGACGCTGTTCATGAGATCCACTCATCTTTAATCCTTGAGGCACCCCAGGGATTTAG
  AGATGAGCCATATCTTGAAGAACTCGTGGATGAACCTTTTCTAGATTTGGGAAAGTCTTTGCAGTTCCAA
  CAAAAAGACGTGGACAGCAGCTCGGAAGCTTGGGAAATGCATGAGTTCCTGAGTCCTCCGCTGGACGGCA
  GGGGTGAGGAGAGAGAGATGCTTGTCGATGAGGAGTATGAACTTTATCAAGATCACCTCCGGGCCATGGA
  GTCAAACCCACCACCACCACACATTCGGGAGGCCGCTCCTGCATCTCCCGTGCTGGAACTCCAGGCTGGT
  ACACAGTGGCTGCATGCTAACCTCTCAGGAGGCGAAAGGCTGGAGTGTCACGATGCAGAGTCCATGATGA
  GTGCCTATCCCCAGGAGATGCAGCAGTATAGCTACAGCACTGCAGACATGGTACGGCTTTGCGACACAGC
  CACCACCTGCTCCCCGCTCTGCCCCCACACCCCTCGCTCATGTTACTTCCATTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222656 representing NM_001005784
  Red=Cloning site Green=Tags(s)
  
  MPENPAAEKMQVLQVLDRLRGKLQEKGDTTQNEKLSAFYETLKSPLFNQILTLQQSIKQLKGQLSHIPSD
  CSANFDFSRKGLLVFTDGSITNGNAQRPCSNVTASELLPWTQKSASEDFNSVIQQMAQGRHVEYIDIERP
  STGGLGFSVVALRSQSLGLIDIFVKEVHPGSVADRDHRLKENDQILAINDTPLDQNISHQQAIALLQQAT
  GSLRLVVAREVGHTQGRASTSSADTTLPETVCWGHTEEVELINDGSGLGFGIVGGKSSGVVVRTIVPGGL
  ADRDGRLQTGDHILKIGGTNVQGMTSEQVAQVLRNCGNSVRMLVARDPVGEIAVTPPTPVSLPVALPAVA
  TRTLDSDRSPFETYSVELVKKDGQSLGIRIVGYVGTAHPGEASGIYVKSIIPGSAAYHNGQIQVNDKIVA
  VDGVNIQGFANQDVVEVLRNAGQVVHLTLVRRKTSLSASPFEHSSSRETVAEPPKVPERAGSPKPEANLS
  VEAEEIGERLDNLKNNTVQALEKPDVYPEKVPGSPENELKSRWENLLGPDYEVMVATLDAQIADDEELQK
  YSKLLPIHTLRLGMEVDSFDGHHYISSVAPGGPVDTLNLLQPEDELLEVNGMQLYGKSRREAVSFLKEVP
  PPFTLVCCRRLFDDEASVDEPRTMEPALLEAEVDHSVDVNIEDDDDGELALWSPEVKTVELVKDCKGLGF
  SILDYQDPLDPTRSVIVIRSLVADGVAERSGELLPGDRLVSVNEFSLDNATLAEAVEVLKAVPPGVVHLG
  ICKPLVEDEKEERFSLHSNNNGDSSEPADAVHEIHSSLILEAPQGFRDEPYLEELVDEPFLDLGKSLQFQ
  QKDVDSSSEAWEMHEFLSPPLDGRGEEREMLVDEEYELYQDHLRAMESNPPPPHIREAAPASPVLELQAG
  TQWLHANLSGGERLECHDAESMMSAYPQEMQQYSYSTADMVRLCDTATTCSPLCPHTPRSCYFHS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005784
• ORF Size: 2925 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005784.1, NP_001005784.1
• RefSeq Size: 3335 bp
• RefSeq ORF: 2928 bp
• Locus ID: 12695
• UniProt ID: Q63ZW7
• Cytogenetics: 4 C6
• Gene Summary: This gene encodes a multivalent PDZ domain protein, which is expressed exclusively in brain and kidney. This protein selectively interacts with inward rectifier K+ (Kir) family members, N-methyl-D-aspartate receptor subunits, neurexins and neuroligins, as well as cell surface molecules enriched in synaptic membranes. Thus, this protein may serve as a scaffold that brings structurally diverse but functionally connected proteins into close proximity at the synapse. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]