Ciita (NM_007575) Mouse Tagged ORF Clone

CAT#: MG222576

Ciita (tGFP-tagged) - Mouse class II transactivator (Ciita), (10ug)

Product Images

Circular map for MG222576

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ciita
• Synonyms: C2t; C2ta; EG669998; Gm9475; Mhc2ta
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222576 representing NM_007575
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTGCCTGGTTCCTGGCCCTTCTGGGTCTTACCTGCCGGAGTTGCAAGACCATAGTCTGTGTGCCA
  CCATGGATCTGGGATCTCCAGAGGGCAGCTACCTGGAACTCCTTAACAGTGATGCCGACCCCCTACATCT
  CTACCACCTCTATGACCAGATGGACCTGGCTGGGGAGGAGGAGATCGAACTCAGCTCAGAGCCAGACACA
  GATACCATCAACTGCGACCAGTTCAGCAAGCTGTTGCAGGACATGGAACTGGATGAAGAGACCCGGGAGG
  CCTATGCCAACATTGCGGAACTGGATCAGTACGTGTTCCAGGATACCCAGCTCGAGGGCCTGAGCAAGGA
  CCTCTTCATAGAGCACATTGGAGCAGAGGAAGGCTTTGGTGAGAACATAGAGATCCCTGTAGAAGCAGGA
  CAGAAGCCTCAGAAGAGACGCTTCCCGGAAGAGCATGCTATGGACTCAAAGCACAGGAAGCTAGTGCCCA
  CCTCTAGGACCTCACTGAACTATTTGGATCTCCCCACTGGGCACATCCAGATCTTCACCACTCTGCCCCA
  GGGACTCTGGCAAATCTCAGGGGCTGGCACAGGTCTCTCCAGTGTCCTAATCTACCACGGTGAGATGCCC
  CAGGTCAACCAAGTGCTCCCTTCAAGCAGCCTCAGTATCCCCAGTCTCCCCGAGTCCCCAGACCGGCCTG
  GCTCCACCAGCCCCTTCACACCATCTGCAGCTGACCTGCCCAGCATGCCCGAACCTGCGCTGACCTCCCG
  TGTAAATGAGACAGAGGACACATCTCCCTCCCCATGCCAAGAGGGTCCCGAGTCTTCCATCAAGCTTCCA
  AAATGGCCAGAGGCTGTGGAGCGATTCCAGCACTCCCTACAGGACAAATACAAGGCATTGCCCCAGAGCC
  CAAGGGGTCCTCTGGTGGCCGTGGAGCTGGTACGGGCCAGGCTGGAAAGAGGCAGCAACAAGAGCCAGGA
  AAGGGAGCTGGCCACTCCCGACTGGACAGAGCGCCAGCTAGCCCACGGTGGTCTGGCAGAGGTACTTCAG
  GTTGTCAGTGACTGCAGGCGACCAGGAGAGACACAGGTGGTCGCTGTGCTGGGCAAGGCTGGCCAGGGAA
  AGAGCCACTGGGCCAGGACAGTGAGTCACACCTGGGCATGTGGCCAGTTGCTACAATATGACTTTGTCTT
  CTATGTCCCCTGTCATTGCTTGGATCGTCCCGGGGACACCTACCACCTGCGGGATCTGCTCTGTCCCCCG
  AGCCTGCAGCCACTGGCCATGGATGACGAGGTCCTTGATTATATCGTGAGGCAGCCAGACCGTGTTCTGC
  TCATCCTAGATGCTTTCGAGGAGCTAGAGGCCCAAGATGGCCTCCTGCACGGACCCTGTGGATCTCTGTC
  CCCAGAGCCCTGCTCCCTCCGAGGACTGCTGGCTGGGATCTTCCAGCGGAAGCTACTGCGAGGCTGCACA
  CTGCTCCTCACAGCCCGGCCCCGGGGCCGCCTGGCTCAGAGCCTGAGCAAGGCAGATGCCATCTTTGAGG
  TGCCCAGCTTCTCTACCAAGCAGGCCAAGACTTACATGAGGCACTACTTTGAGAACTCAGGGACAGCGGG
  GAACCAAGACAAGGCCCTGGGCCTCCTGGAGGGCCAGCCTCTTCTCTGCAGCTATAGTCACAGCCCTGTT
  GTGTGCAGGGCTGTGTGCCAGCTCTCCAAGGCCCTGCTAGAACAGGGCACAGAGGCCCAGCTACCTTGTA
  CACTTACAGGACTCTATGTCAGCCTGCTAGGTCCTGCAGCTCAGAACAGTCCTCCCGGAGCCTTAGTCGA
  GCTGGCCAAGCTGGCCTGGGAGCTGGGACGAAGACACCAAAGCACCTTGCAAGAAACCCGGTTTTCATCC
  GTGGAGGTGAAAACCTGGGCAGTGACCCAAGGCTTGATGCAGCAGACCCTGGAGACCACGGAGGCTCAAC
  TGGCCTTCTCCAGTTTTCTGCTACAGTGTTTCCTGGGTGCTGTGTGGCTGGCACAGTGCAATGAAATCAA
  AGACAAGGAGCTGCCACAGTACCTGGCCTTGACTCCGAGGAAGAAGAGACCCTATGACAACTGGCTGGAG
  GGTGTACCACGCTTTCTGGCTGGATTAGTTTTCCAGCCTCGAGCCCACTGCCTGGGAGCTCTGGTGGAGC
  CTGCAGTGGCTGCAGTGGCGGATAGGAAACAGAAGGTTCTTACCAGGTACCTGAAGCGCCTGAAGCTGGG
  GACACTCCGGGCAGGGAGGCTGCTGGAGCTGCTCCACTGTGCCCACGAGACACAGCAACCTGGGATATGG
  GAGCATGTTGCACACCAGCTCCCTGGCCACCTCTCCTTCCTGGGCACCCGGCTCACACCCCCAGATGTGT
  ATGTGCTGGGCAGGGCCTTGGAGACAGCCAGCCAGGACTTCTCCTTGGACCTTCGTCAGACTGGCGTTGA
  GCCTTCTGGACTGGGAAACCTCGTGGGACTCAGCTGTGTCACCAGTTTCAGGGCCTCCTTGAGTGATACA
  ATGGCATTATGGGAGTCCCTTCAGCAGCAGGGAGAAGCCCAGCTACTCCAGGCGGCAGAGGAGAAGTTCA
  CCATTGAGCCATTTAAAGCCAAATCCCCAAAGGATGTGGAAGACCTGGATCGTCTCGTGCAGACCCAGAG
  GCTGAGAAACCCCTCAGAAGATGCAGCCAAGGATCTTCCTGCCATCCGGGACCTTAAGAAGCTAGAGTTT
  GCGTTGGGCCCCATCTTGGGCCCCCAGGCTTTCCCCACACTGGCAAAGATCCTTCCAGCCTTCTCTTCTC
  TGCAACACCTGGACCTGGACTCACTTAGTGAGAACAAGATCGGAGACAAGGGTGTGTCGAAGCTCTCAGC
  CACCTTCCCTCAGCTGAAGGCCCTGGAGACGCTCAACTTGTCCCAAAACAACATCACTGATGTGGGTGCC
  TGCAAGCTTGCAGAAGCTCTGCCAGCCCTAGCCAAGTCCCTCCTAAGGCTGAGCTTGTACAATAACTGCA
  TCTGTGACAAAGGAGCCAAGAGCCTGGCACAAGTACTTCCGGACATGGTGTCCCTGCGTGTGATGGATGT
  CCAGTTCAACAAGTTCACGGCTGCCGGTGCCCAGCAACTGGCCTCCAGCCTTCAGAAGTGCCCTCAGGTG
  GAAACACTGGCAATGTGGACACCCACTATCCCCTTTGGGGTTCAGGAACACCTGCAGCAGCTGGATGCCA
  GGATCAGTCTGAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222576 representing NM_007575
  Red=Cloning site Green=Tags(s)
  
  MRCLVPGPSGSYLPELQDHSLCATMDLGSPEGSYLELLNSDADPLHLYHLYDQMDLAGEEEIELSSEPDT
  DTINCDQFSKLLQDMELDEETREAYANIAELDQYVFQDTQLEGLSKDLFIEHIGAEEGFGENIEIPVEAG
  QKPQKRRFPEEHAMDSKHRKLVPTSRTSLNYLDLPTGHIQIFTTLPQGLWQISGAGTGLSSVLIYHGEMP
  QVNQVLPSSSLSIPSLPESPDRPGSTSPFTPSAADLPSMPEPALTSRVNETEDTSPSPCQEGPESSIKLP
  KWPEAVERFQHSLQDKYKALPQSPRGPLVAVELVRARLERGSNKSQERELATPDWTERQLAHGGLAEVLQ
  VVSDCRRPGETQVVAVLGKAGQGKSHWARTVSHTWACGQLLQYDFVFYVPCHCLDRPGDTYHLRDLLCPP
  SLQPLAMDDEVLDYIVRQPDRVLLILDAFEELEAQDGLLHGPCGSLSPEPCSLRGLLAGIFQRKLLRGCT
  LLLTARPRGRLAQSLSKADAIFEVPSFSTKQAKTYMRHYFENSGTAGNQDKALGLLEGQPLLCSYSHSPV
  VCRAVCQLSKALLEQGTEAQLPCTLTGLYVSLLGPAAQNSPPGALVELAKLAWELGRRHQSTLQETRFSS
  VEVKTWAVTQGLMQQTLETTEAQLAFSSFLLQCFLGAVWLAQCNEIKDKELPQYLALTPRKKRPYDNWLE
  GVPRFLAGLVFQPRAHCLGALVEPAVAAVADRKQKVLTRYLKRLKLGTLRAGRLLELLHCAHETQQPGIW
  EHVAHQLPGHLSFLGTRLTPPDVYVLGRALETASQDFSLDLRQTGVEPSGLGNLVGLSCVTSFRASLSDT
  MALWESLQQQGEAQLLQAAEEKFTIEPFKAKSPKDVEDLDRLVQTQRLRNPSEDAAKDLPAIRDLKKLEF
  ALGPILGPQAFPTLAKILPAFSSLQHLDLDSLSENKIGDKGVSKLSATFPQLKALETLNLSQNNITDVGA
  CKLAEALPALAKSLLRLSLYNNCICDKGAKSLAQVLPDMVSLRVMDVQFNKFTAAGAQQLASSLQKCPQV
  ETLAMWTPTIPFGVQEHLQQLDARISLR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007575
• ORF Size: 3234 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007575.4, NP_031601.1
• RefSeq Size: 5153 bp
• RefSeq ORF: 3237 bp
• Locus ID: 12265
• UniProt ID: P79621
• Cytogenetics: 16 A1
• Gene Summary: This gene encodes a member of the NOD-like receptor protein family. This protein acts as a transcriptional coactivator and component of the enhanceosome complex to stimulate transcription of MHC class II genes in the adaptive immune response. This protein may also regulate the transcription of MHC class I genes. Mutations in the human gene have been linked to a rare immunodeficiency, bare lymphocyte syndrome, and homozygous knockout mice exhibit many features of this disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]