Rpgr (NM_001177952) Mouse Tagged ORF Clone

CAT#: MG222540

Rpgr (tGFP-tagged) - Mouse retinitis pigmentosa GTPase regulator (Rpgr) transcript variant 4, (10ug)

Product Images

Circular map for MG222540

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rpgr
• Synonyms: Rd9; Rp3h
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222540 representing NM_001177952
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGGCAGTAACAACTGGGGTCAGTTAGGATTAGGATCAAAAGCTGCTATCATCAAGCCAACATGTA
  TCAAAGCTCTTAAGCCTGAGAAGGTGAAACTTGCTGCCTGTGGAAGGAACCACACCTTAGTTTCAACAGA
  TACTGGTGGCGTATATGCAGCTGGTGGAAATAATGAAGGTCAACTGGGGCTTGGTGACACTGACGATAGA
  GACACCTTTCATCAAATTGTCTTCTTTACACCTGCTGATACCATTAAACAGCTCTCTGCTGGCGCCAATA
  CATCCGCTGCTCTTACTGAGGATGGAAAACTTTTTATGTGGGGTGACAATTCTGAAGGGCAGATTGGTCT
  AGAAGATAAAAGTAATGTATGTATCCCTCATGAAGTGACTGTTGGAAAGCCAATTTCCTGGATCTCTTGT
  GGATATTACCATTCAGCTTTTGTAACAATGGATGGGGAGCTCTACACATTTGGAGAACCCGAGAATGGGA
  AGTTGGGCCTTCCCAATGAGCTGCTGATGAATCACAGATCACCCCAGCGTGTGCTGGGCATTCCTGAGAG
  GGTCATTCAAGTGGCCTGTGGTGGAGGGCACACTGTGGTTCTCACAGAGAAAGTTGTGTATGCCTTTGGG
  CTGGGGCAGTTTGGACAACTGGGCCTTGGCACTTTTCTCTTTGAAACATCAGAACCCAAAATTATTGAGC
  GTATTAAGGATCAGAAAATATGTCATATTTCCTGTGGAGAAAACCATACAGCTTTGATGACAGAACTAGG
  CCTCCTGTATACTTTTGGAGACGGCCGACATGGAAAGTTAGGACTTGGGATGGAGAATTTCACCAATCAG
  TTCTTTCCTACCTTGTGCTCTAACTTTTTGAGATTTGCAGTTCAATTGATTGCCTGTGGTGGATGTCATA
  TGCTAGTTTTTGCCACTCCACGACTTGGTACAATAGATGAACCTAAATTTGAAGACGTATATGAGCCTTA
  TATAAGTACAGGTTCTTTTTCCATCAATGACCTCTCCCCAAGAAGTTCACTGAATAGATCTTTATCAGCA
  CGTCTGCGGCGAAGAGAGCGGGAGAGACCCCCATGCTCAGCTTCAATGGTGGGAACACTGCCTCCATTAG
  AGGGGACTTCTGCCTCCACTTCAGCTTATTTTTACCCCAGTTCACCCCCCTTCCATTTGTCTGTGAATAA
  CTACCCAGAGAAAAGCCCCTCTGAATCAATGGAGCCACTGGACTCAGATTATTTTGAAGATAAAATGAAC
  AAAGACACAGAGACAGAAAATTCTTCAGCAGTGGATTCAGAAAACTTTGGTGAAACTAATGATATCTTAA
  ATATGACACATATGATGACTACGAGTTCCAATGAGAAGTTATTAGATTTTTCACCAATTCAAAAACAACA
  GGCTCCTCAACTTTCAGAAACTGTGAAACCAGAAGAAGGGGAAATGGATGAGGAAATTAGTATCCTGAAT
  GTAGAAGACACTGTAGAAGAAGAAAGGAAGGAAGGAGAGAAGGAGATTGTAGAAGAAGGAAGTATACCTG
  AAACAGAAGGCTCAGAAACTATTGATATTACTGATGAGAAGCTAGATGAAGTCCTTAAAGAGGAAGACAG
  TGCCAGCCTTCTGCAACGGGCCCTTCGTGAGTACAATGAAAATCCAAAAGGACACATGTATGATCGTGTA
  AAGAGCAGCTCTTCAGAAATTCTGGGAGGTAATGATCCAACAAGTAAAGACATAAAAAAAGCTAAAAAAA
  TTTCCTTCTTTAATCGGATGTCATTGACAGGTCAGAAACTGATGCAGAATACTAATGATCCACTCCCAGA
  GATAAAGCCAATAGGAGATCAGATAGCCTTACAAAGTGATAAGAAAGATGCCAACCAGAACCACATGGGT
  CAAAATCTTCAGGATTCGACAACACCAAATATGGAGGGAAAGTCCAAATCCTGTACAATTCTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222540 representing NM_001177952
  Red=Cloning site Green=Tags(s)
  
  MFGSNNWGQLGLGSKAAIIKPTCIKALKPEKVKLAACGRNHTLVSTDTGGVYAAGGNNEGQLGLGDTDDR
  DTFHQIVFFTPADTIKQLSAGANTSAALTEDGKLFMWGDNSEGQIGLEDKSNVCIPHEVTVGKPISWISC
  GYYHSAFVTMDGELYTFGEPENGKLGLPNELLMNHRSPQRVLGIPERVIQVACGGGHTVVLTEKVVYAFG
  LGQFGQLGLGTFLFETSEPKIIERIKDQKICHISCGENHTALMTELGLLYTFGDGRHGKLGLGMENFTNQ
  FFPTLCSNFLRFAVQLIACGGCHMLVFATPRLGTIDEPKFEDVYEPYISTGSFSINDLSPRSSLNRSLSA
  RLRRRERERPPCSASMVGTLPPLEGTSASTSAYFYPSSPPFHLSVNNYPEKSPSESMEPLDSDYFEDKMN
  KDTETENSSAVDSENFGETNDILNMTHMMTTSSNEKLLDFSPIQKQQAPQLSETVKPEEGEMDEEISILN
  VEDTVEEERKEGEKEIVEEGSIPETEGSETIDITDEKLDEVLKEEDSASLLQRALREYNENPKGHMYDRV
  KSSSSEILGGNDPTSKDIKKAKKISFFNRMSLTGQKLMQNTNDPLPEIKPIGDQIALQSDKKDANQNHMG
  QNLQDSTTPNMEGKSKSCTIL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177952
• ORF Size: 1953 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177952.1, NP_001171423.1
• RefSeq Size: 2795 bp
• RefSeq ORF: 1956 bp
• Locus ID: 19893
• Cytogenetics: X A1.1
• Gene Summary: Could be a guanine-nucleotide releasing factor (By similarity). Plays a role in ciliogenesis (By similarity). Probably regulates cilia formation by regulating actin stress filaments and cell contractility (By similarity). May be involved in microtubule organization and regulation of transport in primary cilia (By similarity). Plays an important role in photoreceptor integrity. Isoform 5 may play a critical role in spermatogenesis and in intraflagellar transport processes.[UniProtKB/Swiss-Prot Function]