Rpgr (NM_001177952) Mouse Untagged Clone

CAT#: MC220070

Rpgr (untagged) - Mouse retinitis pigmentosa GTPase regulator (Rpgr), transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rpgr
• Synonyms: Rd9; Rp3h
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220070 representing NM_001177952
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGGCAGTAACAACTGGGGTCAGTTAGGATTAGGATCAAAAGCTGCTATCATCAAGCCAACATGTA
  TCAAAGCTCTTAAGCCTGAGAAGGTGAAACTTGCTGCCTGTGGAAGGAACCACACCTTAGTTTCAACAGA
  TACTGGTGGCGTATATGCAGCTGGTGGAAATAATGAAGGTCAACTGGGGCTTGGTGACACTGACGATAGA
  GACACCTTTCATCAAATTGTCTTCTTTACACCTGCTGATACCATTAAACAGCTCTCTGCTGGCGCCAATA
  CATCCGCTGCTCTTACTGAGGATGGAAAACTTTTTATGTGGGGTGACAATTCTGAAGGGCAGATTGGTCT
  AGAAGATAAAAGTAATGTATGTATCCCTCATGAAGTGACTGTTGGAAAGCCAATTTCCTGGATCTCTTGT
  GGATATTACCATTCAGCTTTTGTAACAATGGATGGGGAGCTCTACACATTTGGAGAACCCGAGAATGGGA
  AGTTGGGCCTTCCCAATGAGCTGCTGATGAATCACAGATCACCCCAGCGTGTGCTGGGCATTCCTGAGAG
  GGTCATTCAAGTGGCCTGTGGTGGAGGGCACACTGTGGTTCTCACAGAGAAAGTTGTGTATGCCTTTGGG
  CTGGGGCAGTTTGGACAACTGGGCCTTGGCACTTTTCTCTTTGAAACATCAGAACCCAAAATTATTGAGC
  GTATTAAGGATCAGAAAATATGTCATATTTCCTGTGGAGAAAACCATACAGCTTTGATGACAGAACTAGG
  CCTCCTGTATACTTTTGGAGACGGCCGACATGGAAAGTTAGGACTTGGGATGGAGAATTTCACCAATCAG
  TTCTTTCCTACCTTGTGCTCTAACTTTTTGAGATTTGCAGTTCAATTGATTGCCTGTGGTGGATGTCATA
  TGCTAGTTTTTGCCACTCCACGACTTGGTACAATAGATGAACCTAAATTTGAAGACGTATATGAGCCTTA
  TATAAGTACAGGTTCTTTTTCCATCAATGACCTCTCCCCAAGAAGTTCACTGAATAGATCTTTATCAGCA
  CGTCTGCGGCGAAGAGAGCGGGAGAGACCCCCATGCTCAGCTTCAATGGTGGGAACACTGCCTCCATTAG
  AGGGGACTTCTGCCTCCACTTCAGCTTATTTTTACCCCAGTTCACCCCCCTTCCATTTGTCTGTGAATAA
  CTACCCAGAGAAAAGCCCCTCTGAATCAATGGAGCCACTGGACTCAGATTATTTTGAAGATAAAATGAAC
  AAAGACACAGAGACAGAAAATTCTTCAGCAGTGGATTCAGAAAACTTTGGTGAAACTAATGATATCTTAA
  ATATGACACATATGATGACTACGAGTTCCAATGAGAAGTTATTAGATTTTTCACCAATTCAAAAACAACA
  GGCTCCTCAACTTTCAGAAACTGTGAAACCAGAAGAAGGGGAAATGGATGAGGAAATTAGTATCCTGAAT
  GTAGAAGACACTGTAGAAGAAGAAAGGAAGGAAGGAGAGAAGGAGATTGTAGAAGAAGGAAGTATACCTG
  AAACAGAAGGCTCAGAAACTATTGATATTACTGATGAGAAGCTAGATGAAGTCCTTAAAGAGGAAGACAG
  TGCCAGCCTTCTGCAACGGGCCCTTCGTGAGTACAATGAAAATCCAAAAGGACACATGTATGATCGTGTA
  AAGAGCAGCTCTTCAGAAATTCTGGGAGGTAATGATCCAACAAGTAAAGACATAAAAAAAGCTAAAAAAA
  TTTCCTTCTTTAATCGGATGTCATTGACAGGTCAGAAACTGATGCAGAATACTAATGATCCACTCCCAGA
  GATAAAGCCAATAGGAGATCAGATAGCCTTACAAAGTGATAAGAAAGATGCCAACCAGAACCACATGGGT
  CAAAATCTTCAGGATTCGACAACACCAAATATGGAGGGAAAGTCCAAATCCTGTACAATTCTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177952
• Insert Size: 1956 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177952.1, NP_001171423.1
• RefSeq Size: 2795 bp
• RefSeq ORF: 1956 bp
• Locus ID: 19893
• Cytogenetics: X A1.1
• Gene Summary: Could be a guanine-nucleotide releasing factor (By similarity). Plays a role in ciliogenesis (By similarity). Probably regulates cilia formation by regulating actin stress filaments and cell contractility (By similarity). May be involved in microtubule organization and regulation of transport in primary cilia (By similarity). Plays an important role in photoreceptor integrity. Isoform 5 may play a critical role in spermatogenesis and in intraflagellar transport processes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and initiates translation at a downstream start codon, and lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The resulting protein (isoform 4) is shorter and has a distinct N-terminus compared to isoform 1.