Ablim1 (NM_001103178) Mouse Tagged ORF Clone

CAT#: MG222518

Ablim1 (tGFP-tagged) - Mouse actin-binding LIM protein 1 (Ablim1) transcript variant 3, (10ug)

Product Images

Circular map for MG222518

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ablim1
• Synonyms: 2210411C18Rik; 2610209L21Rik; 4833406P10Rik; 9330196J19Rik; AV079770; AW060987
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222518 representing NM_001103178
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACAGAAGGGGAAGAAATGTATCTGCAAGGTTCTACCGTGTGGCATCCCGACTGTAAGCAGTCCA
  CTAAGACAGAGGAGAAGCTGCGGCCACCAAACATCCCGCGCTCTTCATCCGATTTCTTTTACCCCAAAAG
  TCTGATTCGTCGCACAGGACGGTCCCCAGCTCTGCAGCTTCTGTCGCCACCCTGTCTGATGAACTCCAAC
  AAAAACCCCAGGCAGCCCACCAGGACCTCCTCTGAAAGTATCTATTCTAGACCAGGCTCCAGCATCCCTG
  GTTCACCAGGCCATACTATCTATGCAAAAGTAGACAATGAAATCCTGGATTACAAGGATTTAGCAGCCAT
  CCCCAAGGTCAAGGCGATCTATGACATCGAGCGTCCAGATCTCATTACCTATGAGCCTTTCTACACATCA
  GGCTATGAGGACAAGCAGGAGAGACAGAGCCTTGGAGAGTCTCCAAGGACTTTGTCTCCAACTCCATCTG
  CAGAAGGTTATCAAGATGTCCGGGATCGGATGATCCACAGGTCCACCAGCCAGGGCTCCATCAACTCCCC
  TGTGTATAGCCGGCACAGTTACACTCCAACTACGTCTCGCTCGCCCCAGCACTTCCACCGACCTGAGCTC
  TTGTCTCCTGGTGTGCACAGGTGGTCTCCCCTGCGCACCAGCAGCTTCAGCTCCACCCACAGCGACTCCC
  GTCCCAACCCCCCCTTCCGACACCACTTCCTCCCCCATGTCAAAGGCAATGAGCCGTCCAGCGGCCGGAA
  CTCCCCTCTCCCCTACCGGCCCGACAGCCGCCCTCTAACTCCAACTTACGCTCAGGCCCCTAAACATTTC
  CATGTTCCAGATCAAGGGATCAACATTTACCGAAAACCACCCATCTACAAACAGCATGCTGCCTTGGCAG
  CCCAGAGCAAGGCTTCAGAAGATATCATCAAGTTTTCCAAGTTCCCAGCAGCCCAGGCGCCAGATCCCAA
  CGAGATACCAAAGATTGAGACGGACCACTGGCCGGGTCCCCCCTCACTTGCCGCCGTAGGAACTGACCCG
  AGGCGCAGATCAAGTGGCAGAGAGGAAGATGAAGAGGAGCTTTTGAGACGCCGGCAGCTTCAAGAAGAAC
  AGTTGATGAAGCTCAACTCAGGCCTGGGGCAGCTGATCCTGAAGGAAGAGATGGAGAAGGAGAGCAGAGA
  AAGAGCATCGCTGGCCAGTCGCTATGACTCTCCCCTCCACTCAGCTTCCCATGCTCCATCATCTAAAACT
  TCATCTCTCCCTGGTTATGGAAAGAATGGCCTTCACCGGCCAGTTTCCACAGACTTCGCTCAGTACAACA
  GCTACGGAGACATCAGTGGCGGAGTTCGAGACTACCAGACCCTCCCAGATGGCCACATGCCTGCAGTGAG
  AATGGACAGAGGAGTATCCATGCCCAACATGTTGGAACCAAAGATATTTCCATATGAGATGCTCATGGTG
  ACCAACAGAGGGCGCAACAAAATCCTGAGAGATGTGGATAGAACCCGGCTAGAGCGCCACTTAGCCCCAG
  AAGTATTTTGGGAGATCTTTGGGATGTCCATACAGGAATTTGACAAGTTACCTCTTTGGAGACGCAACGA
  CATGAAGAAAAAAGCTAAACTCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222518 representing NM_001103178
  Red=Cloning site Green=Tags(s)
  
  MFTEGEEMYLQGSTVWHPDCKQSTKTEEKLRPPNIPRSSSDFFYPKSLIRRTGRSPALQLLSPPCLMNSN
  KNPRQPTRTSSESIYSRPGSSIPGSPGHTIYAKVDNEILDYKDLAAIPKVKAIYDIERPDLITYEPFYTS
  GYEDKQERQSLGESPRTLSPTPSAEGYQDVRDRMIHRSTSQGSINSPVYSRHSYTPTTSRSPQHFHRPEL
  LSPGVHRWSPLRTSSFSSTHSDSRPNPPFRHHFLPHVKGNEPSSGRNSPLPYRPDSRPLTPTYAQAPKHF
  HVPDQGINIYRKPPIYKQHAALAAQSKASEDIIKFSKFPAAQAPDPNEIPKIETDHWPGPPSLAAVGTDP
  RRRSSGREEDEEELLRRRQLQEEQLMKLNSGLGQLILKEEMEKESRERASLASRYDSPLHSASHAPSSKT
  SSLPGYGKNGLHRPVSTDFAQYNSYGDISGGVRDYQTLPDGHMPAVRMDRGVSMPNMLEPKIFPYEMLMV
  TNRGRNKILRDVDRTRLERHLAPEVFWEIFGMSIQEFDKLPLWRRNDMKKKAKLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001103178
• ORF Size: 1635 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001103178.3
• RefSeq Size: 5927 bp
• RefSeq ORF: 1638 bp
• Locus ID: 226251
• UniProt ID: Q8K4G5
• Cytogenetics: 19 52.09 cM
• Gene Summary: May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.[UniProtKB/Swiss-Prot Function]