Ablim1 (NM_001103178) Mouse Untagged Clone

CAT#: MC218885

Ablim1 (untagged) - Mouse actin-binding LIM protein 1 (Ablim1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ablim1
• Synonyms: 2210411C18Rik; 2610209L21Rik; 4833406P10Rik; 9330196J19Rik; AV079770; AW060987
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218885 representing NM_001103178
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACAGAAGGGGAAGAAATGTATCTGCAAGGTTCTACCGTGTGGCATCCCGACTGTAAGCAGTCCA
  CTAAGACAGAGGAGAAGCTGCGGCCACCAAACATCCCGCGCTCTTCATCCGATTTCTTTTACCCCAAAAG
  TCTGATTCGTCGCACAGGACGGTCCCCAGCTCTGCAGCTTCTGTCGCCACCCTGTCTGATGAACTCCAAC
  AAAAACCCCAGGCAGCCCACCAGGACCTCCTCTGAAAGTATCTATTCTAGACCAGGCTCCAGCATCCCTG
  GTTCACCAGGCCATACTATCTATGCAAAAGTAGACAATGAAATCCTGGATTACAAGGATTTAGCAGCCAT
  CCCCAAGGTCAAGGCGATCTATGACATCGAGCGTCCAGATCTCATTACCTATGAGCCTTTCTACACATCA
  GGCTATGAGGACAAGCAGGAGAGACAGAGCCTTGGAGAGTCTCCAAGGACTTTGTCTCCAACTCCATCTG
  CAGAAGGTTATCAAGATGTCCGGGATCGGATGATCCACAGGTCCACCAGCCAGGGCTCCATCAACTCCCC
  TGTGTATAGCCGGCACAGTTACACTCCAACTACGTCTCGCTCGCCCCAGCACTTCCACCGACCTGAGCTC
  TTGTCTCCTGGTGTGCACAGGTGGTCTCCCCTGCGCACCAGCAGCTTCAGCTCCACCCACAGCGACTCCC
  GTCCCAACCCCCCCTTCCGACACCACTTCCTCCCCCATGTCAAAGGCAATGAGCCGTCCAGCGGCCGGAA
  CTCCCCTCTCCCCTACCGGCCCGACAGCCGCCCTCTAACTCCAACTTACGCTCAGGCCCCTAAACATTTC
  CATGTTCCAGATCAAGGGATCAACATTTACCGAAAACCACCCATCTACAAACAGCATGCTGCCTTGGCAG
  CCCAGAGCAAGGCTTCAGAAGATATCATCAAGTTTTCCAAGTTCCCAGCAGCCCAGGCGCCAGATCCCAA
  CGAGATACCAAAGATTGAGACGGACCACTGGCCGGGTCCCCCCTCACTTGCCGCCGTAGGAACTGACCCG
  AGGCGCAGATCAAGTGGCAGAGAGGAAGATGAAGAGGAGCTTTTGAGACGCCGGCAGCTTCAAGAAGAAC
  AGTTGATGAAGCTCAACTCAGGCCTGGGGCAGCTGATCCTGAAGGAAGAGATGGAGAAGGAGAGCAGAGA
  AAGAGCATCGCTGGCCAGTCGCTATGACTCTCCCCTCCACTCAGCTTCCCATGCTCCATCATCTAAAACT
  TCATCTCTCCCTGGTTATGGAAAGAATGGCCTTCACCGGCCAGTTTCCACAGACTTCGCTCAGTACAACA
  GCTACGGAGACATCAGTGGCGGAGTTCGAGACTACCAGACCCTCCCAGATGGCCACATGCCTGCAGTGAG
  AATGGACAGAGGAGTATCCATGCCCAACATGTTGGAACCAAAGATATTTCCATATGAGATGCTCATGGTG
  ACCAACAGAGGGCGCAACAAAATCCTGAGAGATGTGGATAGAACCCGGCTAGAGCGCCACTTAGCCCCAG
  AAGTATTTTGGGAGATCTTTGGGATGTCCATACAGGAATTTGACAAGTTACCTCTTTGGAGACGCAACGA
  CATGAAGAAAAAAGCTAAACTCTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001103178
• Insert Size: 1638 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001103178.2, NP_001096648.1
• RefSeq Size: 5942 bp
• RefSeq ORF: 1638 bp
• Locus ID: 226251
• UniProt ID: Q8K4G5
• Cytogenetics: 19 52.09 cM
• Gene Summary: May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks multiple 5' coding exons, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3, also known as abLIM-s) has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.