Sorbs1 (NM_001034962) Mouse Tagged ORF Clone

CAT#: MG222352

Sorbs1 (tGFP-tagged) - Mouse sorbin and SH3 domain containing 1 (Sorbs1) transcript variant 3, (10ug)

Product Images

Circular map for MG222352

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sorbs1
• Synonyms: 2310065E01Rik; 9530001P15Rik; CAP; mKIAA1296; Sh3d5; SH3P12
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222352 representing NM_001034962
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCTGAATGTGATGTTGGAAGCTCTAAAGCTGTGGTGAATGGCTTGGCATCTGGCAACCATGGAC
  CAGACAAAGCCGACCCTTTCCGTGCTCGCTCTATATCTGCTGTTAAAATAATTCCCGTGAAGACAGTGAA
  AAGCCCTTCAGGCCTAGTACTCCCTCCAGACATGGACCCTACCAAAATCTGCACTGGGAAAGGAACAGTG
  ACTCTTCGGGCCTCGTCTTCCTACAGGGGAACCCCAAGCAGCAGCCCTGTGAGCCCCCAGGAATCTCCGA
  AGCATGAAAGCAAGTCAGATGAATGGAAACTTTCTTCCAGTGCAGATACCAATGGCAACGCCCAGCCCTC
  CCCACTTGCTGCCAAGGGCTATAGAAGTGTGCATCCCAGCCTTTCTGCTGACAAGCCCCAGGGCAGTCCT
  TTACTAAACGAAGTTTCTTCTTCCCACATTGAAACCGATTCCCAAGACTTCCCTCCAACAAGCAGACCTT
  CGTCTGCCTACCCCTCCACCACCATCGTCAACCCTACCATTGTGCTCCTGCAGCACAATCGAGAGCAGCA
  AAAGCGACTCAGTAGTCTTTCAGATCCTGCCTCAGAGAGAAGAGCGGGTGAGCAGGACCCAGTACCAACC
  CCAGCAGAACTCACTTCGCCCGGCAGGGCTTCTGAGAGAAGGGCAAAGGATGCTAGCAGACGGGTGGTGA
  GGAGCACACAGGACCTGAGCGATGTGTCTACAGATGAAGTGGGCATCCCACTCCGGAATACCGAGCGATC
  GAAAGACTGGTACAAAACTATGTTTAAACAGATCCACAAACTGAACAGAGATGATGATTCTGATGTCCAT
  TCCCCTCGATATTCCTTCTCTGACGACACAAAGTCTCCCCTTTCTGTGCCTCGCTCAAAAAGTGAGATGA
  ACTACATCGAAGGGGAGAAAGTGGTTAAGAGGTCCGCCACACTCCCGCTCCCAGCCCGCTCCTCCTCACT
  CAAGTCCAGCCCGGAAAGAAACGACTGGGAGCCCCCAGATAAGAAAGTGGATACGAGAAAATACCGAGCA
  GAGCCCAAAAGCATTTACGAATATCAGCCGGGCAAGTCTTCGGTCCTGACCAATGAGAAGATGAGCTCAG
  CAGTCAGCCCGACTCCAGACATTACGTCAGAGCCTCCTGGATATATCTATTCTTCCAACTTCCATGCAGT
  GAAGAGAGAATCGGACGGGACCCCCGGGGGTCTCGCTAGCTTGGAGAATGAGAGGCAGATCTATAAGAGT
  GTCTTGGAAGGTGGCGACATCCCTCTTCAGGGCCTCAGTGGGCTCAAGCGACCTTCCAGCTCAGCTTCCA
  CTAAAGATTCAGAGTCACCAAGACATTTTATACCAGCTGATTACTTGGAGTCCACAGAAGAATTTATTCG
  GAGACGGCACGATGATAAAGAGATGAGACCTGCTCGAGCCAAATTTGACTTTAAAGCCCAGACCCTGAAG
  GAGCTGCCTCTGCAGAAGGGAGACGTTGTTTACATCTACAGACAGATTGACCAGAACTGGTATGAAGGTG
  AACACCATGGCCGGGTGGGAATCTTCCCACGCACCTATATCGAGCTTCTTCCTCCAGCTGAGAAGGCTCA
  GCCCAGAAAGTTGGCACCCGTACAAGTTTTGGAATATGGAGAAGCCATTGCGAAGTTTAACTTTAATGGA
  GATACACAAGTAGAAATGTCTTTCCGAAAGGGGGAGAGGATCACGCTGCTCCGACAGGTGGATGAGAACT
  GGTACGAAGGGAGGATTCCTGGGACATCTCGCCAAGGCATTTTCCCTATCACCTATGTAGATGTGCTTAA
  GAGGCCATTGGTGAAAACCCCTGTGGATTACATCGACCTGCCTTATTCTTCTTCCCCAAGTCGCAGTGCC
  ACTGTGAGCCCACAGCAACCTCAAGCCCAGCAGCGAAGAGTCACCCCAGACAGGAGTCAGCCCTCACTGG
  ATTTGTGTAGCTACCAAGCGTTATATAGTTATGTGCCACAGAACGATGATGAGTTGGAACTCCGAGATGG
  AGATATTGTTGATGTCATGGAAAAATGTGACGATGGATGGTTTGTTGGCACTTCGAGAAGGACGAGGCAG
  TTTGGTACTTTTCCAGGCAACTATGTAAAACCTTTATATCTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222352 representing NM_001034962
  Red=Cloning site Green=Tags(s)
  
  MSSECDVGSSKAVVNGLASGNHGPDKADPFRARSISAVKIIPVKTVKSPSGLVLPPDMDPTKICTGKGTV
  TLRASSSYRGTPSSSPVSPQESPKHESKSDEWKLSSSADTNGNAQPSPLAAKGYRSVHPSLSADKPQGSP
  LLNEVSSSHIETDSQDFPPTSRPSSAYPSTTIVNPTIVLLQHNREQQKRLSSLSDPASERRAGEQDPVPT
  PAELTSPGRASERRAKDASRRVVRSTQDLSDVSTDEVGIPLRNTERSKDWYKTMFKQIHKLNRDDDSDVH
  SPRYSFSDDTKSPLSVPRSKSEMNYIEGEKVVKRSATLPLPARSSSLKSSPERNDWEPPDKKVDTRKYRA
  EPKSIYEYQPGKSSVLTNEKMSSAVSPTPDITSEPPGYIYSSNFHAVKRESDGTPGGLASLENERQIYKS
  VLEGGDIPLQGLSGLKRPSSSASTKDSESPRHFIPADYLESTEEFIRRRHDDKEMRPARAKFDFKAQTLK
  ELPLQKGDVVYIYRQIDQNWYEGEHHGRVGIFPRTYIELLPPAEKAQPRKLAPVQVLEYGEAIAKFNFNG
  DTQVEMSFRKGERITLLRQVDENWYEGRIPGTSRQGIFPITYVDVLKRPLVKTPVDYIDLPYSSSPSRSA
  TVSPQQPQAQQRRVTPDRSQPSLDLCSYQALYSYVPQNDDELELRDGDIVDVMEKCDDGWFVGTSRRTRQ
  FGTFPGNYVKPLYL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034962
• ORF Size: 2142 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034962.1, NP_001030134.1
• RefSeq Size: 5282 bp
• RefSeq ORF: 2145 bp
• Locus ID: 20411
• UniProt ID: Q62417
• Cytogenetics: 19 34.25 cM
• Gene Summary: Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions.[UniProtKB/Swiss-Prot Function]