Kdm3a (NM_173001) Mouse Tagged ORF Clone

CAT#: MG222266

Kdm3a (tGFP-tagged) - Mouse lysine (K)-specific demethylase 3A (Kdm3a) transcript variant 1, (10ug)

Product Images

Circular map for MG222266

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kdm3a
• Synonyms: 1700105C21Rik; C230043E16Rik; JHDM2a; Jmjd1; Jmjd1a; KDM2A; TGSA; Tsga
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222266 representing NM_173001
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTCACGCTCGGAGAAAGTTGGCCAGTATTGGTGGGGAAGCGATTCCTCAGTCTGTCCGCAGCCG
  AAGGCAACGAAGGCGGCCAGGACAACTGGGACTTGGAGCGCGTTGCCGAGTGGCCCTGGCTGTCGGGGAC
  CATTCGAGCTGTTTCCCACACCGACGTTACTAAGAAAGACTTGAAGGTGTGTGTGGAGTTTGATGGGGAG
  TCTTGGAGAAAGAGAAGATGGATAGATGTCTACAGCCTTCAGAGAAAAGCATTTTTAGTAGAGCATAACC
  TGGTTTTGGCAGAACGAAAATCACCTGAAGTTCCTGAGCAAGTTATTCAGTGGCCTGCAATAATGTACAA
  ATCTCTTCTAGACAAAGCTGGCTTGGGAGCCATAACTTCTGTTCGGTTTCTTGGAGATCAACAAAGTGTA
  TTTGTTTCCAAAGACCTTTTGAAACCTATACAGGATGTTAACAGTCTTCGGCTTTCCCTTACTGATAATC
  AGACAGTCAGTAAGGAATTTCAAGCTTTGATTGTAAAACATTTGGATGAAAGCCATCTTTTACAAGGTGA
  CAAGAACCTTGTTGGTTCAGAAGTAAAAATTTATAGCTTGGACCCATCTACTCAGTGGTTTTCAGCAACT
  GTTGTACATGGAAACCCATCATCCAAAACTCTTCAAGTCAACTGTGAGGAGATTCCAGCACTGAAAATTG
  TCGACCCAGCACTGATTCATGTTGAAGTTGTACATGACAACTTTGTGACATGTGGTAATTCTACAAGAAC
  TGGAGCTGTAAAACGCAAGTCTTCTGAGAATAACGGAAGTTCGGTTTCTAAACAAGCAAAATCTTGTTCT
  GAGGCCTCTCCCAGTATGTGTCCTGTACAGTCTGTTCCCACAACAGTGTTTAAGGAGATCCTGCTTGGCT
  GTACTGCAGCAACTCCATCTAGCAAGGACCCAAGACAGCAAAATACTCCCCAGGCAGCCAATTCTCCACC
  TAACATTGGAGCAAAACTTCCTCAAGGATGTCATAAGCAGAACTTACCAGAAGAACTTTCTTCCTGTCTA
  AACACAAAACCTGAAGTACCGAGAACAAAACCAGATGTCTGCAAAGAAGGATTACTTTCTTCAAAATCTT
  CTCAGGTTGGAGCTGGAGACTTGAAAATTCTGAGTGAGCCCAAAGGTAGCTGTATCCAGCCTAAAACAAA
  CACTGATCAGGAGAGCAGACTGGAGTCTGCTCCACAGCCAGTCACTGGCCTTCCAAAGGAGTGCTTGCCT
  GCAAAGACTTCCTCTAAGGCAGAACTGGACATTGCCACCACTCCTGAACTGCAGAAGCATCTAGAACATG
  CAGCTTCCACATCCGATGACCTTTCAGATAAGCCAGAAGTGAAAGCAGGTGTCACTAGCCTTAATAGTTG
  TGCAGAAAAGAAGGTCGAACCTTCACATTTAGGTTCCCAGTCACAGAATTTAAAGGAAACTTCAGTAAAA
  GTAGATAATGAAAGCTGTTGTACAAGAAGCAGTAATAAAACCCAGACTCCCCCAGCCCGGAAGTCAGTTT
  TGACAGACCCAGATAAAGTCAGGAAGCTGCAGCAGAGCGGAGAGGCCTTTGTTCAGGATGACTCCTGTGT
  TAACATCGTGGCACAGCTGCCCAAGTGTCGGGAGTGTCGACTAGACAGCCTGCGCAAGGATAAGGACCAG
  CAGAAGGACTCTCCTGTGTTTTGTCGCTTTTTCCACTTCAGGAGATTACAATTCAACAAGCATGGTGTGT
  TGCGGGTAGAAGGCTTCTTAACACCAAACAAGTATGACAGTGAAGCGATTGGCTTGTGGCTGCCTTTGAC
  CAAAAATGTTGTGGGGACTGATTTGGACACAGCAAAATATATCCTGGCCAATATTGGAGACCACTTCTGT
  CAAATGGTGATTTCTGAGAAGGAAGCTATGTCGACTATTGAGCCACACAGACAGGTTGCTTGGAAACGAG
  CTGTCAAAGGAGTTAGAGAAATGTGTGATGTGTGTGACACAACCATTTTCAACCTGCACTGGGTGTGCCC
  TCGGTGTGGGTTTGGAGTATGTGTAGATTGCTACCGGATGAAGAGGAAGAACTGCCAACAGGGTGCTGCC
  TACAAGACTTTCTCTTGGATAAGGTGTGTGAAGAGTCAGATACATGAGCCTGAGAACCTGATGCCCACAC
  AGATTATTCCTGGCAAAGCCCTCTACGATGTTGGAGACATTGTGCATTCTGTCAGAGCAAAATGGGGCAT
  AAAGGCCAATTGTCCCTGCTCCAACAGGCAGTTCAAGCTCTTCTCAAAGCCAGCCTTAAAGGAAGACCTG
  AAACAGACATCCTTGTCTGGAGAAAAACCAACTCTTGGGACCATGGTCCAGCAAAGTTCCCCTGTTTTGG
  AGCCAGTGGCTGTGTGCGGGGAAGCAGCCTCCAAGCCAGCCAGCAGCGTGAAGCCCACCTGTCCCACCAG
  CACTTCACCTTTAAACTGGCTAGCTGACCTTACCAGTGGGAATGTCAACAAGGAGAATAAGGAAAAACAG
  CTGACTATGCCAATTTTAAAGAATGAAATCAAATGCCTTCCACCCTTGCCCCCTCTGAACAAGCCCAGCA
  CAGTCCTCCATACTTTTAACAGCACCATCTTGACACCTGTGAGCAACAATAATTCAGGTTTCCTTAGAAA
  TCTTTTGAATTCATCCACAGCAAAGACAGAAAATGGATTGAAAAACACACCCAAAATTCTTGATGACATC
  TTTGCCTCTTTGGTGCAAAACAAGACTTCTTCTGATTCATCCAAGAGGCCTCAAGGACTGACAATCAAGC
  CTAGCATTCTTGGCTTTGACACTCCTCACTACTGGCTGTGTGACAACCGCCTGCTGTGCTTGCAAGACCC
  CAACAATAAGAGCAATTGGAATGTTTTTAGGGAATGCTGGAAACAAGGGCAGCCAGTGATGGTGTCGGGC
  GTGCATCATAAATTAAACACTGAACTCTGGAAACCCGAGTCCTTCAGAAAAGAGTTTGGTGAGCAGGAAG
  TAGACCTAGTCAATTGTAGGACCAATGAAATCATCACTGGAGCCACAGTCGGAGACTTCTGGGATGGATT
  TGAAGATGTTCCAAACCGTTTGAAAAACGACAAAGAAAAAGAACCAATGGTGTTGAAACTTAAGGACTGG
  CCGCCAGGAGAAGACTTCAGAGACATGATGCCTTCCAGGTTTGATGATCTGATGGCCAACATTCCACTGC
  CTGAGTACACCAGGCGAGATGGCAAACTGAACCTGGCTTCCAGACTGCCAAACTACTTTGTACGGCCAGA
  CCTGGGCCCCAAGATGTACAATGCTTATGGATTGATCACTCCAGAGGATCGGAAATATGGGACCACAAAT
  CTTCACTTAGATGTATCTGATGCAGCCAATGTCATGGTTTATGTGGGAATTCCCAAAGGACAGTGTGAAC
  AAGAAGAAGAAGTCCTTAGAACCATCCAAGATGGAGATTCTGATGAACTCACAATCAAGAGATTTATTGA
  AGGAAAAGAGAAGCCAGGAGCCCTTTGGCACATATATGCTGCTAAAGACACAGAGAAGATAAGAGAATTC
  CTTAAAAAGGTATCAGAGGAGCAGGGTCAAGACAACCCTGCAGACCATGACCCTATCCACGATCAGAGCT
  GGTATTTAGACCGATCGCTGAGAAAGCGCCTCTATCAAGAGTACGGCGTGCAAGGCTGGGCTATTGTACA
  GTTTCTTGGGGATGTGGTGTTTATCCCAGCAGGAGCGCCACATCAGGTTCATAACTTATACAGCTGTATC
  AAAGTGGCTGAAGACTTTGTGTCTCCAGAGCATGTTAAACACTGCTTCTGGCTTACTCAGGAATTCCGTT
  ACTTGTCACAGACTCATACCAACCATGAAGATAAATTGCAGGTGAAAAATGTTATCTACCATGCAGTGAA
  AGATGCAGTTGCTATGCTGAAAGCCAGTGAATCCAGTTTGGGCAAACCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222266 representing NM_173001
  Red=Cloning site Green=Tags(s)
  
  MVLTLGESWPVLVGKRFLSLSAAEGNEGGQDNWDLERVAEWPWLSGTIRAVSHTDVTKKDLKVCVEFDGE
  SWRKRRWIDVYSLQRKAFLVEHNLVLAERKSPEVPEQVIQWPAIMYKSLLDKAGLGAITSVRFLGDQQSV
  FVSKDLLKPIQDVNSLRLSLTDNQTVSKEFQALIVKHLDESHLLQGDKNLVGSEVKIYSLDPSTQWFSAT
  VVHGNPSSKTLQVNCEEIPALKIVDPALIHVEVVHDNFVTCGNSTRTGAVKRKSSENNGSSVSKQAKSCS
  EASPSMCPVQSVPTTVFKEILLGCTAATPSSKDPRQQNTPQAANSPPNIGAKLPQGCHKQNLPEELSSCL
  NTKPEVPRTKPDVCKEGLLSSKSSQVGAGDLKILSEPKGSCIQPKTNTDQESRLESAPQPVTGLPKECLP
  AKTSSKAELDIATTPELQKHLEHAASTSDDLSDKPEVKAGVTSLNSCAEKKVEPSHLGSQSQNLKETSVK
  VDNESCCTRSSNKTQTPPARKSVLTDPDKVRKLQQSGEAFVQDDSCVNIVAQLPKCRECRLDSLRKDKDQ
  QKDSPVFCRFFHFRRLQFNKHGVLRVEGFLTPNKYDSEAIGLWLPLTKNVVGTDLDTAKYILANIGDHFC
  QMVISEKEAMSTIEPHRQVAWKRAVKGVREMCDVCDTTIFNLHWVCPRCGFGVCVDCYRMKRKNCQQGAA
  YKTFSWIRCVKSQIHEPENLMPTQIIPGKALYDVGDIVHSVRAKWGIKANCPCSNRQFKLFSKPALKEDL
  KQTSLSGEKPTLGTMVQQSSPVLEPVAVCGEAASKPASSVKPTCPTSTSPLNWLADLTSGNVNKENKEKQ
  LTMPILKNEIKCLPPLPPLNKPSTVLHTFNSTILTPVSNNNSGFLRNLLNSSTAKTENGLKNTPKILDDI
  FASLVQNKTSSDSSKRPQGLTIKPSILGFDTPHYWLCDNRLLCLQDPNNKSNWNVFRECWKQGQPVMVSG
  VHHKLNTELWKPESFRKEFGEQEVDLVNCRTNEIITGATVGDFWDGFEDVPNRLKNDKEKEPMVLKLKDW
  PPGEDFRDMMPSRFDDLMANIPLPEYTRRDGKLNLASRLPNYFVRPDLGPKMYNAYGLITPEDRKYGTTN
  LHLDVSDAANVMVYVGIPKGQCEQEEEVLRTIQDGDSDELTIKRFIEGKEKPGALWHIYAAKDTEKIREF
  LKKVSEEQGQDNPADHDPIHDQSWYLDRSLRKRLYQEYGVQGWAIVQFLGDVVFIPAGAPHQVHNLYSCI
  KVAEDFVSPEHVKHCFWLTQEFRYLSQTHTNHEDKLQVKNVIYHAVKDAVAMLKASESSLGKP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173001
• ORF Size: 3969 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173001.3, NP_766589.1
• RefSeq Size: 4868 bp
• RefSeq ORF: 3972 bp
• Locus ID: 104263
• UniProt ID: Q6PCM1
• Cytogenetics: 6 C1
• Gene Summary: Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation (By similarity). Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin (PubMed:17943087). Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.[UniProtKB/Swiss-Prot Function]