Kdm3a (NM_173001) Mouse Untagged Clone

CAT#: MC224246

Kdm3a (untagged) - Mouse lysine (K)-specific demethylase 3A (Kdm3a), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kdm3a
• Synonyms: 1700105C21Rik; C230043E16Rik; JHDM2a; Jmjd1; Jmjd1a; KDM2A; TGSA; Tsga
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224246 representing NM_173001
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTCACGCTCGGAGAAAGTTGGCCAGTATTGGTGGGGAAGCGATTCCTCAGTCTGTCCGCAGCCG
  AAGGCAACGAAGGCGGCCAGGACAACTGGGACTTGGAGCGCGTTGCCGAGTGGCCCTGGCTGTCGGGGAC
  CATTCGAGCTGTTTCCCACACCGACGTTACTAAGAAAGACTTGAAGGTGTGTGTGGAGTTTGATGGGGAG
  TCTTGGAGAAAGAGAAGATGGATAGATGTCTACAGCCTTCAGAGAAAAGCATTTTTAGTAGAGCATAACC
  TGGTTTTGGCAGAACGAAAATCACCTGAAGTTCCTGAGCAAGTTATTCAGTGGCCTGCAATAATGTACAA
  ATCTCTTCTAGACAAAGCTGGCTTGGGAGCCATAACTTCTGTTCGGTTTCTTGGAGATCAACAAAGTGTA
  TTTGTTTCCAAAGACCTTTTGAAACCTATACAGGATGTTAACAGTCTTCGGCTTTCCCTTACTGATAATC
  AGACAGTCAGTAAGGAATTTCAAGCTTTGATTGTAAAACATTTGGATGAAAGCCATCTTTTACAAGGTGA
  CAAGAACCTTGTTGGTTCAGAAGTAAAAATTTATAGCTTGGACCCATCTACTCAGTGGTTTTCAGCAACT
  GTTGTACATGGAAACCCATCATCCAAAACTCTTCAAGTCAACTGTGAGGAGATTCCAGCACTGAAAATTG
  TCGACCCAGCACTGATTCATGTTGAAGTTGTACATGACAACTTTGTGACATGTGGTAATTCTACAAGAAC
  TGGAGCTGTAAAACGCAAGTCTTCTGAGAATAACGGAAGTTCGGTTTCTAAACAAGCAAAATCTTGTTCT
  GAGGCCTCTCCCAGTATGTGTCCTGTACAGTCTGTTCCCACAACAGTGTTTAAGGAGATCCTGCTTGGCT
  GTACTGCAGCAACTCCATCTAGCAAGGACCCAAGACAGCAAAATACTCCCCAGGCAGCCAATTCTCCACC
  TAACATTGGAGCAAAACTTCCTCAAGGATGTCATAAGCAGAACTTACCAGAAGAACTTTCTTCCTGTCTA
  AACACAAAACCTGAAGTACCGAGAACAAAACCAGATGTCTGCAAAGAAGGATTACTTTCTTCAAAATCTT
  CTCAGGTTGGAGCTGGAGACTTGAAAATTCTGAGTGAGCCCAAAGGTAGCTGTATCCAGCCTAAAACAAA
  CACTGATCAGGAGAGCAGACTGGAGTCTGCTCCACAGCCAGTCACTGGCCTTCCAAAGGAGTGCTTGCCT
  GCAAAGACTTCCTCTAAGGCAGAACTGGACATTGCCACCACTCCTGAACTGCAGAAGCATCTAGAACATG
  CAGCTTCCACATCCGATGACCTTTCAGATAAGCCAGAAGTGAAAGCAGGTGTCACTAGCCTTAATAGTTG
  TGCAGAAAAGAAGGTCGAACCTTCACATTTAGGTTCCCAGTCACAGAATTTAAAGGAAACTTCAGTAAAA
  GTAGATAATGAAAGCTGTTGTACAAGAAGCAGTAATAAAACCCAGACTCCCCCAGCCCGGAAGTCAGTTT
  TGACAGACCCAGATAAAGTCAGGAAGCTGCAGCAGAGCGGAGAGGCCTTTGTTCAGGATGACTCCTGTGT
  TAACATCGTGGCACAGCTGCCCAAGTGTCGGGAGTGTCGACTAGACAGCCTGCGCAAGGATAAGGACCAG
  CAGAAGGACTCTCCTGTGTTTTGTCGCTTTTTCCACTTCAGGAGATTACAATTCAACAAGCATGGTGTGT
  TGCGGGTAGAAGGCTTCTTAACACCAAACAAGTATGACAGTGAAGCGATTGGCTTGTGGCTGCCTTTGAC
  CAAAAATGTTGTGGGGACTGATTTGGACACAGCAAAATATATCCTGGCCAATATTGGAGACCACTTCTGT
  CAAATGGTGATTTCTGAGAAGGAAGCTATGTCGACTATTGAGCCACACAGACAGGTTGCTTGGAAACGAG
  CTGTCAAAGGAGTTAGAGAAATGTGTGATGTGTGTGACACAACCATTTTCAACCTGCACTGGGTGTGCCC
  TCGGTGTGGGTTTGGAGTATGTGTAGATTGCTACCGGATGAAGAGGAAGAACTGCCAACAGGGTGCTGCC
  TACAAGACTTTCTCTTGGATAAGGTGTGTGAAGAGTCAGATACATGAGCCTGAGAACCTGATGCCCACAC
  AGATTATTCCTGGCAAAGCCCTCTACGATGTTGGAGACATTGTGCATTCTGTCAGAGCAAAATGGGGCAT
  AAAGGCCAATTGTCCCTGCTCCAACAGGCAGTTCAAGCTCTTCTCAAAGCCAGCCTTAAAGGAAGACCTG
  AAACAGACATCCTTGTCTGGAGAAAAACCAACTCTTGGGACCATGGTCCAGCAAAGTTCCCCTGTTTTGG
  AGCCAGTGGCTGTGTGCGGGGAAGCAGCCTCCAAGCCAGCCAGCAGCGTGAAGCCCACCTGTCCCACCAG
  CACTTCACCTTTAAACTGGCTAGCTGACCTTACCAGTGGGAATGTCAACAAGGAGAATAAGGAAAAACAG
  CTGACTATGCCAATTTTAAAGAATGAAATCAAATGCCTTCCACCCTTGCCCCCTCTGAACAAGCCCAGCA
  CAGTCCTCCATACTTTTAACAGCACCATCTTGACACCTGTGAGCAACAATAATTCAGGTTTCCTTAGAAA
  TCTTTTGAATTCATCCACAGCAAAGACAGAAAATGGATTGAAAAACACACCCAAAATTCTTGATGACATC
  TTTGCCTCTTTGGTGCAAAACAAGACTTCTTCTGATTCATCCAAGAGGCCTCAAGGACTGACAATCAAGC
  CTAGCATTCTTGGCTTTGACACTCCTCACTACTGGCTGTGTGACAACCGCCTGCTGTGCTTGCAAGACCC
  CAACAATAAGAGCAATTGGAATGTTTTTAGGGAATGCTGGAAACAAGGGCAGCCAGTGATGGTGTCGGGC
  GTGCATCATAAATTAAACACTGAACTCTGGAAACCCGAGTCCTTCAGAAAAGAGTTTGGTGAGCAGGAAG
  TAGACCTAGTCAATTGTAGGACCAATGAAATCATCACTGGAGCCACAGTCGGAGACTTCTGGGATGGATT
  TGAAGATGTTCCAAACCGTTTGAAAAACGACAAAGAAAAAGAACCAATGGTGTTGAAACTTAAGGACTGG
  CCGCCAGGAGAAGACTTCAGAGACATGATGCCTTCCAGGTTTGATGATCTGATGGCCAACATTCCACTGC
  CTGAGTACACCAGGCGAGATGGCAAACTGAACCTGGCTTCCAGACTGCCAAACTACTTTGTACGGCCAGA
  CCTGGGCCCCAAGATGTACAATGCTTATGGATTGATCACTCCAGAGGATCGGAAATATGGGACCACAAAT
  CTTCACTTAGATGTATCTGATGCAGCCAATGTCATGGTTTATGTGGGAATTCCCAAAGGACAGTGTGAAC
  AAGAAGAAGAAGTCCTTAGAACCATCCAAGATGGAGATTCTGATGAACTCACAATCAAGAGATTTATTGA
  AGGAAAAGAGAAGCCAGGAGCCCTTTGGCACATATATGCTGCTAAAGACACAGAGAAGATAAGAGAATTC
  CTTAAAAAGGTATCAGAGGAGCAGGGTCAAGACAACCCTGCAGACCATGACCCTATCCACGATCAGAGCT
  GGTATTTAGACCGATCGCTGAGAAAGCGCCTCTATCAAGAGTACGGCGTGCAAGGCTGGGCTATTGTACA
  GTTTCTTGGGGATGTGGTGTTTATCCCAGCAGGAGCGCCACATCAGGTTCATAACTTATACAGCTGTATC
  AAAGTGGCTGAAGACTTTGTGTCTCCAGAGCATGTTAAACACTGCTTCTGGCTTACTCAGGAATTCCGTT
  ACTTGTCACAGACTCATACCAACCATGAAGATAAATTGCAGGTGAAAAATGTTATCTACCATGCAGTGAA
  AGATGCAGTTGCTATGCTGAAAGCCAGTGAATCCAGTTTGGGCAAACCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173001
• Insert Size: 3972 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173001.3, NP_766589.1
• RefSeq Size: 4857 bp
• RefSeq ORF: 3972 bp
• Locus ID: 104263
• UniProt ID: Q6PCM1
• Cytogenetics: 6 C1
• Gene Summary: Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation (By similarity). Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin (PubMed:17943087). Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 all encode the same isoform (a).