Bicd2 (NM_029791) Mouse Tagged ORF Clone

CAT#: MG221687

Bicd2 (tGFP-tagged) - Mouse bicaudal D homolog 2 (Drosophila) (Bicd2) transcript variant 2, (10ug)

Product Images

Circular map for MG221687

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bicd2
• Synonyms: 0610027D24Rik; 1110005D12Rik; AA408834; mKIAA0699
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221687 representing NM_029791
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCGCCGTCGGAGGAGGAGGAGTATGCGCGTCTGGTGATGGAGGCGCAGCCGGAGTGGCTGCGCG
  CCGAGGTGAAGCGGCTGTCCCACGAGCTGGCCGAGACCACGCGTGAGAAGATCCAGGCGGCCGAGTATGG
  ACTGGCGGTGCTGGAGGAGAAGCACCAGCTCAAGCTGCAGTTCGAGGAGCTCGAGGTGGACTATGAGGCC
  ATCCGCAGCGAGATGGAGCAGCTCAAAGAGGCATTTGGCCAGGCACATACAAACCACAAGAAGGTGGCTG
  CTGATGGTGAGAGCCGGGAGGAGAGCCTGATCCAGGAGTCGGCCTCCAAGGAGCAGTACTACGTGCGGAA
  GGTGCTGGAGCTGCAGACAGAGCTGAAACAGCTGCGCAACGTCCTCACCAACACCCAGTCTGAGAATGAG
  CGCCTCACGTCTGTGGCCCAGGAGCTAAAAGAGATCAACCAGAATGTGGAGATCCAGCGTGGTCGCCTGC
  GGGATGACATCAAGGAGTACAAGTTCCGGGAGGCCCGTCTACTTCAGGACTACTCAGAGCTGGAGGAGGA
  GAACATCAGCCTGCAGAAACAAGTGTCTGTGCTCAGGCAGAACCAGGTGGAGTTTGAGGGCCTCAAGCAT
  GAAATCAAGCGCCTGGAGGAGGAGACAGAGTACCTCAACAGCCAGCTAGAGGATGCCATCCGGCTTAAGG
  AGATCTCTGAACGGCAGTTGGAGGAGGCGCTGGAGACACTGAAGACAGAGCGGGAGCAGAAGAACAACCT
  GCGCAAGGAGTTGTCGCACTACATGAGCATCAACGATTCCTTCTATACCAGCCACCTGCAGGTCTCCTTA
  GATGGCCTCAAGTTCAGTGATGATACTGTCACCGCAGAGCCCAACAATGACGCCGAAGCCCTGGTCAATG
  GCTTTGAGCACAGTGGCTTGGTCAAATCATCCTTGGACAACAAGACATCCACACCCAGGAAGGATGGCCT
  AGCTCCACCCTCCCCCAGCCTCGTCTCTGACCTGCTCAGTGAGCTCCACATATCTGAGATCCAGAAGCTG
  AAACAGCAGCTGGTGCAGATGGAGCGGGAGAAGGTGGGCCTGCTGGCAACACTGCAGGACACACAGAAGC
  AGCTGGAGCAGGCTCGGGGAACCCTCTCTGAGCAGCATGAGAAGGTGAATCGTCTCACAGAGAACCTTAG
  TGCCCTCCGGCGCCTGCAGGCTGGCAAGGAGCGACAGACCTCGTTGGATAATGAGAAGGACCGTGACAGC
  CACGAAGACGGTGACTACTATGAGGTGGACATCAATGGGCCTGAGATCCTGGCCTGCAAGTACCACGTGG
  CTGTGGCTGAGGCTGGCGAGCTCCGGGAGCAGCTCAAGGCGTTGCGCAGCACACACGAAGCTCGCGAAGC
  CCAGCACGCAGAAGAAAAGGGCCGGTATGAGGCTGAGGGCCAGGCACTCACTGAGAAGATCTCTCTGCTG
  GAGAAGGCTAGCCACCAGGACCGAGAGCTGCTGGCCCATCTGGAAAAGGAGCTGAAGAAGGTGAGCGATG
  TGGCTGGTGAGACCCAGGGCAGCCTGAATGTGGCTCAGGATGAGCTGGTGACCTTCAGCGAGGAGCTGGC
  CAACCTCTACCACCATGTGTGCATGTGCAACAACGAGACGCCCAACCGTGTCATGCTCGACTATTATCGT
  GAGGGCCAGGGCAAGGCTGGCCGCACCAGCCCAGAGGGCCGCGGGCGCCGGTCACCTGTCCTCTTGCCCA
  AGGGGCTGTTGGCCACAGAGGTGGGCCGAGCAGATGGTGGGACTGGGGACAACAGTCCTTCACCCAGTTC
  TTCACTGCCGTCACCTCTGAGTGACCCCCGCCGGGAACCTATGAACATCTACAACCTGATCGCCATCATC
  CGAGACCAGATCAAGCACCTGCAGGCAGCCGTGGACCGTACGACCGAGCTGTCCCGGCAGCGGATTGCCT
  CGCAGGAGCTGGGCCCTGCTGTGGACAAGGACAAGGAAGCACTCATGGAGGAGATCCTCAAGCTGAAGTC
  CCTGCTGAGTACCAAGCGGGAGCAGATCACCACACTGCGCACCGTGCTCAAGGCCAACAAGCAGACAGCT
  GAGGTGGCCCTGGCCAACCTGAAGAGCAAGTATGAGAACGAGAAGGCCATGGTGACCGAGACCATGATGA
  AGCTACGGAACGAGCTCAAGGCCCTCAAGGAAGACGCAGCCACCTTCTCCTCCCTGCGTGCCATGTTTGC
  CACCAGGTGTGACGAATACATCACACAGCTGGATGAGATGCAGCGGCAGCTGGCAGCTGCCGAGGACGAG
  AAGAAGACCCTTAACTCTCTGTTGCGCATGGCCATCCAGCAGAAGCTGGCGCTCACCCAGCGGCTGGAGC
  TGCTGGAGCTGGACCATGAACAGACCCGCAGGGGCCGCAGCAAGGCCGCCTCCAAGGCCAAGCCAGCCTC
  ACCGAGCCTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221687 representing NM_029791
  Red=Cloning site Green=Tags(s)
  
  MSAPSEEEEYARLVMEAQPEWLRAEVKRLSHELAETTREKIQAAEYGLAVLEEKHQLKLQFEELEVDYEA
  IRSEMEQLKEAFGQAHTNHKKVAADGESREESLIQESASKEQYYVRKVLELQTELKQLRNVLTNTQSENE
  RLTSVAQELKEINQNVEIQRGRLRDDIKEYKFREARLLQDYSELEEENISLQKQVSVLRQNQVEFEGLKH
  EIKRLEEETEYLNSQLEDAIRLKEISERQLEEALETLKTEREQKNNLRKELSHYMSINDSFYTSHLQVSL
  DGLKFSDDTVTAEPNNDAEALVNGFEHSGLVKSSLDNKTSTPRKDGLAPPSPSLVSDLLSELHISEIQKL
  KQQLVQMEREKVGLLATLQDTQKQLEQARGTLSEQHEKVNRLTENLSALRRLQAGKERQTSLDNEKDRDS
  HEDGDYYEVDINGPEILACKYHVAVAEAGELREQLKALRSTHEAREAQHAEEKGRYEAEGQALTEKISLL
  EKASHQDRELLAHLEKELKKVSDVAGETQGSLNVAQDELVTFSEELANLYHHVCMCNNETPNRVMLDYYR
  EGQGKAGRTSPEGRGRRSPVLLPKGLLATEVGRADGGTGDNSPSPSSSLPSPLSDPRREPMNIYNLIAII
  RDQIKHLQAAVDRTTELSRQRIASQELGPAVDKDKEALMEEILKLKSLLSTKREQITTLRTVLKANKQTA
  EVALANLKSKYENEKAMVTETMMKLRNELKALKEDAATFSSLRAMFATRCDEYITQLDEMQRQLAAAEDE
  KKTLNSLLRMAIQQKLALTQRLELLELDHEQTRRGRSKAASKAKPASPSL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029791
• ORF Size: 2460 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029791.4
• RefSeq Size: 4634 bp
• RefSeq ORF: 2463 bp
• Locus ID: 76895
• UniProt ID: Q921C5
• Cytogenetics: 13 A5
• Gene Summary: Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates and stabilizes the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:11483508, PubMed:25035494, PubMed:24986880, PubMed:22956769). Facilitates the binding of RAB6A to the Golgi by stabilizing its GTP-bound form (PubMed:25962623). Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport via its interaction with RAB6A and recruitment of the dynein-dynactin motor complex (PubMed:12447383, PubMed:25962623). Contributes to nuclear and centrosomal positioning prior to mitotic entry through regulation of both dynein and kinesin-1. During G2 phase of the cell cycle, associates with RANBP2 at the nuclear pores and recruits dynein and dynactin to the nuclear envelope to ensure proper positioning of the nucleus relative to centrosomes prior to the onset of mitosis (PubMed:20386726).[UniProtKB/Swiss-Prot Function]