Bicd2 (NM_029791) Mouse Untagged Clone

CAT#: MC201761

Bicd2 (untagged) - Mouse bicaudal D homolog 2 (Drosophila) (Bicd2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bicd2
• Synonyms: 0610027D24Rik; 1110005D12Rik; AA408834; mKIAA0699
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC032198 sequence for NM_029791
  CCACGCGTCCGGCAGCGGCGGCTAAGAGGGCCCGGCTTGTGGGAGCCAAGGTGGGAGCCGGCCTCGGCGC GCGGCCACCATGTCCGCGCCGTCGGAGGAGGAGGAGTATGCGCGTCTGGTGATGGAGGCGCAGCCGGAGT GGCTGCGCGCCGAGGTGAAGCGGCTGTCCCACGAGCTGGCCGAGACCACGCGTGAGAAGATCCAGGCGGC CGAGTATGGACTGGCGGTGCTGGAGGAGAAGCACCAGCTCAAGCTGCAGTTCGAGGAGCTCGAGGTGGAC TATGAGGCCATCCGCAGCGAGATGGAGCAGCTCAAAGAGGCATTTGGCCAGGCACATACAAACCACAAGA AGGTGGCTGCTGATGGTGAGAGCCGGGAGGAGAGCCTGATCCAGGAGTCGGCCTCCAAGGAGCAGTACTA CGTGCGGAAGGTGCTGGAGCTGCAGACAGAGCTGAAACAGCTGCGCAACGTCCTCACCAACACCCAGTCT GAGAATGAGCGCCTCACGTCTGTGGCCCAGGAGCTAAAAGAGATCAACCAGAATGTGGAGATCCAGCGTG GTCGCCTGCGGGATGACATCAAGGAGTACAAGTTCCGGGAGGCCCGTCTACTTCAGGACTACTCAGAGCT GGAGGAGGAGAACATCAGCCTGCAGAAACAAGTGTCTGTGCTCAGGCAGAACCAGGTGGAGTTTGAGGGC CTCAAGCATGAAATCAAGCGCCTGGAGGAGGAGACAGAGTACCTCAACAGCCAGCTAGAGGATGCCATCC GGCTTAAGGAGATCTCTGAACGGCAGTTGGAGGAGGCGCTGGAGACACTGAAGACAGAGCGGGAGCAGAA GAACAACCTGCGCAAGGAGTTGTCGCACTACATGAGCATCAACGATTCCTTCTATACCAGCCACCTGCAG GTCTCCTTAGATGGCCTCAAGTTCAGTGATGATACTGTCACCGCAGAGCCCAACAATGACGCCGAAGCCC TGGTCAATGGCTTTGAGCACAGTGGCTTGGTCAAATCATCCTTGGACAACAAGACATCCACACCCAGGAA GGATGGCCTAGCTCCACCCTCCCCCAGCCTCGTCTCTGACCTGCTCAGTGAGCTCCACATATCTGAGATC CAGAAGCTGAAACAGCAGCTGGTGCAGATGGAGCGGGAGAAGGTGGGCCTGCTGGCAACACTGCAGGACA CACAGAAGCAGCTGGAGCAGGCTCGGGGAACCCTCTCTGAGCAGCATGAGAAGGTGAATCGTCTCACAGA GAACCTTAGTGCCCTCCGGCGCCTGCAGGCTGGCAAGGAGCGACAGACCTCGTTGGATAATGAGAAGGAC CGTGACAGCCACGAAGACGGTGACTACTATGAGGTGGACATCAATGGGCCTGAGATCCTGGCCTGCAAGT ACCACGTGGCTGTGGCTGAGGCTGGCGAGCTCCGGGAGCAGCTCAAGGCGTTGCGCAGCACACACGAAGC TCGCGAAGCCCAGCACGCAGAAGAAAAGGGCCGGTATGAGGCTGAGGGCCAGGCACTCACTGAGAAGATC TCTCTGCTGGAGAAGGCTAGCCACCAGGACCGAGAGCTGCTGGCCCATCTGGAAAAGGAGCTGAAGAAGG TGAGCGATGTGGCTGGTGAGACCCAGGGCAGCCTGAATGTGGCTCAGGATGAGCTGGTGACCTTCAGCGA GGAGCTGGCCAACCTCTACCACCATGTGTGCATGTGCAACAACGAGACGCCCAACCGTGTCATGCTCGAC TATTATCGTGAGGGCCAGGGCAAGGCTGGCCGCACCAGCCCAGAGGGCCGCGGGCGCCGGTCACCTGTCC TCTTGCCCAAGGGGCTGTTGGCCACAGAGGTGGGCCGAGCAGATGGTGGGACTGGGGACAACAGTCCTTC ACCCAGTTCTTCACTGCCGTCACCTCTGAGTGACCCCCGCCGGGAACCTATGAACATCTACAACCTGATC GCCATCATCCGAGACCAGATCAAGCACCTGCAGGCAGCCGTGGACCGTACGACCGAGCTGTCCCGGCAGC GGATTGCCTCGCAGGAGCTGGGCCCTGCTGTGGACAAGGACAAGGAAGCACTCATGGAGGAGATCCTCAA GCTGAAGTCCCTGCTGAGTACCAAGCGGGAGCAGATCACCACACTGCGCACCGTGCTCAAGGCCAACAAG CAGACAGCTGAGGTGGCCCTGGCCAACCTGAAGAGCAAGTATGAGAACGAGAAGGCCATGGTGACCGAGA CCATGATGAAGCTACGGAACGAGCTCAAGGCCCTCAAGGAAGACGCAGCCACCTTCTCCTCCCTGCGTGC CATGTTTGCCACCAGGTGTGACGAATACATCACACAGCTGGATGAGATGCAGCGGCAGCTGGCAGCTGCC GAGGACGAGAAGAAGACCCTTAACTCTCTGTTGCGCATGGCCATCCAGCAGAAGCTGGCGCTCACCCAGC GGCTGGAGCTGCTGGAGCTGGACCATGAACAGACCCGCAGGGGCCGCAGCAAGGCCGCCTCCAAGGCCAA GCCAGCCTCACCGAGCCTGTAGAGTAGCTGCTGGGAGGAGGCGGCCACCCGGCCCTGTCACACAGCAGCC CCTCCCCCTCCCCTCCTGTGGCCCATGCAGAGGAAGGAAGGGCAGCCTAAAGTCCACTTAGAAATGTTTG GATATGCCACTGTAATTCTTTTCAAAATAGCATTCCCCAAGTTTTTAATGGGAGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_029791
• Insert Size: 2463 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC032198, AAH32198
• RefSeq Size: 2729 bp
• RefSeq ORF: 2463 bp
• Locus ID: 76895
• UniProt ID: Q921C5
• Cytogenetics: 13 A5
• Gene Summary: Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates and stabilizes the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:11483508, PubMed:25035494, PubMed:24986880, PubMed:22956769). Facilitates the binding of RAB6A to the Golgi by stabilizing its GTP-bound form (PubMed:25962623). Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport via its interaction with RAB6A and recruitment of the dynein-dynactin motor complex (PubMed:12447383, PubMed:25962623). Contributes to nuclear and centrosomal positioning prior to mitotic entry through regulation of both dynein and kinesin-1. During G2 phase of the cell cycle, associates with RANBP2 at the nuclear pores and recruits dynein and dynactin to the nuclear envelope to ensure proper positioning of the nucleus relative to centrosomes prior to the onset of mitosis (PubMed:20386726).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' coding sequence and 3' UTR, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.