Celf2 (NM_001110230) Mouse Tagged ORF Clone

CAT#: MG221685

Celf2 (tGFP-tagged) - Mouse CUGBP Elav-like family member 2 (Celf2) transcript variant 3, (10ug)

Product Images

Circular map for MG221685

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Celf2
• Synonyms: B230218O03; B230345P09Rik; C88023; CELF-2; CUG-BP2; Cugbp2; D230046B21Rik; Etr-3; mETR-3; Napor
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221685 representing NM_001110230
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTCCCTAATCTCTGACCTGGACTCCCTGAGAGGCTGGAAAGCGTTAAGAGAGACTGCCACAGAGT
  TATCTGGAAGTCCACCAAGTAACGGCACAGCCAACAAGATGAACGGAGCTTTGGATCATTCAGACCAGCC
  AGACCCAGATGCCATTAAGATGTTTGTCGGACAGATCCCTAGGTCCTGGTCGGAAAAGGAGCTGAAAGAA
  CTTTTTGAGCCTTATGGAGCTGTCTACCAGATCAACGTCCTCCGGGACCGGAGTCAGAACCCTCCCCAGA
  GTAAAGGTTGTTGTTTCGTAACATTTTATACAAGAAAAGCTGCACTTGAGGCCCAGAATGCACTGCACAA
  TATTAAAACTTTACCTGGGATGCATCATCCCATTCAGATGAAACCTGCAGATAGTGAAAAGTCAAACGCT
  GTGGAAGACAGAAAATTGTTCATAGGAATGGTTTCCAAGAAATGTAACGAGAATGATATCAGAGTGATGT
  TTTCTCCATTCGGTCAGATAGAAGAATGCCGGATTCTCCGGGGACCTGATGGGCTGAGTCGAGGCTGTGC
  GTTTGTCACATTTTCTACAAGGGCAATGGCACAGAATGCAATCAAAGCCATGCATCAGTCTCAGACCATG
  GAGGGCTGCTCTTCACCAATCGTGGTGAAGTTTGCTGACACTCAGAAGGACAAAGAGCAAAGGCGCCTCC
  AGCAGCAGCTTGCACAGCAGATGCAACAGCTCAACACTGCCACTTGGGGGAACCTAACAGGACTGGGTGG
  ACTTACCCCGCAGTACCTGGCGCTTCTGCAGCAGGCCACCTCCTCCAGCAACCTGGGTGCATTCAGTGGC
  ATTCAGCAAATGGCTGGCATGAATGCTTTACAGTTACAGAATCTGGCAACACTGGCTGCTGCTGCAGCTG
  CTGCTCAAACCTCAGCCACCAGCACCAATGCAAACCCTCTGTCTAGCACAAGCAGTGCCCTGGGAGCCCT
  CACAAGCCCTGTGGCTGCTTCAACCCCCAATTCCACCGCTGGTGCGGCCATGAATTCCTTGACCTCTCTT
  GGGACTCTACAAGGATTGGCTGGAGCCACTGTCGGATTGAATAATATTAATGCACTAGCAGGTATGGCGG
  CTCTGAATGGAGGACTTGGCGCCACAGGCTTGACGAATGGTACGGCTGGCACCATGGACGCCCTGACCCA
  GGCCTACTCAGGAATTCAGCAGTATGCGGCAGCTGCACTGCCCACTTTGTACAGCCAGAGCTTGCTGCAA
  CAGCAGAGTGCTGCAGGCAGCCAGAAGGAAGGTCCAGAGGGGGCAAACCTCTTTATTTACCACCTTCCAC
  AGGAGTTTGGAGACCAGGACATTCTGCAGATGTTCATGCCCTTTGGAAATGTTATCTCTGCTAAAGTCTT
  CATTGACAAACAGACCAATCTGAGCAAGTGCTTTGGTTTTGTTAGCTATGACAATCCAGTCTCTGCACAA
  GCTGCAATCCAGGCTATGAACGGCTTTCAGATCGGCATGAAACGCTTGAAGGTGCAGCTGAAACGCTCCA
  AAAACGACAGCAAACCTTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221685 representing NM_001110230
  Red=Cloning site Green=Tags(s)
  
  MVSLISDLDSLRGWKALRETATELSGSPPSNGTANKMNGALDHSDQPDPDAIKMFVGQIPRSWSEKELKE
  LFEPYGAVYQINVLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNIKTLPGMHHPIQMKPADSEKSNA
  VEDRKLFIGMVSKKCNENDIRVMFSPFGQIEECRILRGPDGLSRGCAFVTFSTRAMAQNAIKAMHQSQTM
  EGCSSPIVVKFADTQKDKEQRRLQQQLAQQMQQLNTATWGNLTGLGGLTPQYLALLQQATSSSNLGAFSG
  IQQMAGMNALQLQNLATLAAAAAAAQTSATSTNANPLSSTSSALGALTSPVAASTPNSTAGAAMNSLTSL
  GTLQGLAGATVGLNNINALAGMAALNGGLGATGLTNGTAGTMDALTQAYSGIQQYAAAALPTLYSQSLLQ
  QQSAAGSQKEGPEGANLFIYHLPQEFGDQDILQMFMPFGNVISAKVFIDKQTNLSKCFGFVSYDNPVSAQ
  AAIQAMNGFQIGMKRLKVQLKRSKNDSKPY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110230
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110230.1, NP_001103700.1
• RefSeq Size: 7749 bp
• RefSeq ORF: 1563 bp
• Locus ID: 14007
• UniProt ID: Q9Z0H4
• Cytogenetics: 2 A1
• Gene Summary: RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing (By similarity). Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle (By similarity). Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB (By similarity). Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs (By similarity). Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA (By similarity). Involved in the apoB RNA editing activity (By similarity). Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury. Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression. Binds to (CUG)n triplet repeats in the 3' UTR of transcripts such as DMPK (By similarity). Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5 (By similarity). Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs (By similarity). Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine (By similarity). Binds AU-rich sequences in the 3' UTR of COX2 mRNA. Binds to an intronic RNA element responsible for the silencing of exon 21 splicing. Binds to (CUG)n repeats. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF1, negatively regulates the processing to mature miRNA (By similarity).[UniProtKB/Swiss-Prot Function]