Celf2 (NM_001110230) Mouse Untagged Clone

CAT#: MC217421

Celf2 (untagged) - Mouse CUGBP, Elav-like family member 2 (Celf2), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Celf2
• Synonyms: B230218O03; B230345P09Rik; C88023; CELF-2; CUG-BP2; Cugbp2; D230046B21Rik; Etr-3; mETR-3; Napor
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217421 representing NM_001110230
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTCCCTAATCTCTGACCTGGACTCCCTGAGAGGCTGGAAAGCGTTAAGAGAGACTGCCACAGAGT
  TATCTGGAAGTCCACCAAGTAACGGCACAGCCAACAAGATGAACGGAGCTTTGGATCATTCAGACCAGCC
  AGACCCAGATGCCATTAAGATGTTTGTCGGACAGATCCCTAGGTCCTGGTCGGAAAAGGAGCTGAAAGAA
  CTTTTTGAGCCTTATGGAGCTGTCTACCAGATCAACGTCCTCCGGGACCGGAGTCAGAACCCTCCCCAGA
  GTAAAGGTTGTTGTTTCGTAACATTTTATACAAGAAAAGCTGCACTTGAGGCCCAGAATGCACTGCACAA
  TATTAAAACTTTACCTGGGATGCATCATCCCATTCAGATGAAACCTGCAGATAGTGAAAAGTCAAACGCT
  GTGGAAGACAGAAAATTGTTCATAGGAATGGTTTCCAAGAAATGTAACGAGAATGATATCAGAGTGATGT
  TTTCTCCATTCGGTCAGATAGAAGAATGCCGGATTCTCCGGGGACCTGATGGGCTGAGTCGAGGCTGTGC
  GTTTGTCACATTTTCTACAAGGGCAATGGCACAGAATGCAATCAAAGCCATGCATCAGTCTCAGACCATG
  GAGGGCTGCTCTTCACCAATCGTGGTGAAGTTTGCTGACACTCAGAAGGACAAAGAGCAAAGGCGCCTCC
  AGCAGCAGCTTGCACAGCAGATGCAACAGCTCAACACTGCCACTTGGGGGAACCTAACAGGACTGGGTGG
  ACTTACCCCGCAGTACCTGGCGCTTCTGCAGCAGGCCACCTCCTCCAGCAACCTGGGTGCATTCAGTGGC
  ATTCAGCAAATGGCTGGCATGAATGCTTTACAGTTACAGAATCTGGCAACACTGGCTGCTGCTGCAGCTG
  CTGCTCAAACCTCAGCCACCAGCACCAATGCAAACCCTCTGTCTAGCACAAGCAGTGCCCTGGGAGCCCT
  CACAAGCCCTGTGGCTGCTTCAACCCCCAATTCCACCGCTGGTGCGGCCATGAATTCCTTGACCTCTCTT
  GGGACTCTACAAGGATTGGCTGGAGCCACTGTCGGATTGAATAATATTAATGCACTAGCAGGTATGGCGG
  CTCTGAATGGAGGACTTGGCGCCACAGGCTTGACGAATGGTACGGCTGGCACCATGGACGCCCTGACCCA
  GGCCTACTCAGGAATTCAGCAGTATGCGGCAGCTGCACTGCCCACTTTGTACAGCCAGAGCTTGCTGCAA
  CAGCAGAGTGCTGCAGGCAGCCAGAAGGAAGGTCCAGAGGGGGCAAACCTCTTTATTTACCACCTTCCAC
  AGGAGTTTGGAGACCAGGACATTCTGCAGATGTTCATGCCCTTTGGAAATGTTATCTCTGCTAAAGTCTT
  CATTGACAAACAGACCAATCTGAGCAAGTGCTTTGGTTTTGTTAGCTATGACAATCCAGTCTCTGCACAA
  GCTGCAATCCAGGCTATGAACGGCTTTCAGATCGGCATGAAACGCTTGAAGGTGCAGCTGAAACGCTCCA
  AAAACGACAGCAAACCTTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001110230
• Insert Size: 1563 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110230.1, NP_001103700.1
• RefSeq Size: 7749 bp
• RefSeq ORF: 1563 bp
• Locus ID: 14007
• UniProt ID: Q9Z0H4
• Cytogenetics: 2 A1
• Gene Summary: RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing (By similarity). Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle (By similarity). Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB (By similarity). Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs (By similarity). Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA (By similarity). Involved in the apoB RNA editing activity (By similarity). Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury. Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression. Binds to (CUG)n triplet repeats in the 3' UTR of transcripts such as DMPK (By similarity). Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5 (By similarity). Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs (By similarity). Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine (By similarity). Binds AU-rich sequences in the 3' UTR of COX2 mRNA. Binds to an intronic RNA element responsible for the silencing of exon 21 splicing. Binds to (CUG)n repeats. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF1, negatively regulates the processing to mature miRNA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and has multiple differences in the presence and absence of exons at its 5' end, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.