Nos1ap (NM_027528) Mouse Tagged ORF Clone

CAT#: MG219444

Nos1ap (tGFP-tagged) - Mouse nitric oxide synthase 1 (neuronal) adaptor protein (Nos1ap) transcript variant 2, (10ug)

Product Images

Circular map for MG219444

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nos1ap
• Synonyms: 6330408P19Rik; Capon
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219444 representing NM_027528
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCAGAATCCGGTATGAGTTTAAAGCCAAGAACATCAAGAAGAAGAAAGTAAGCATCATGGTCTCTG
  TGGACGGTGTCAAGGTGATTCTGAAGAAGAAGAAAAAGAAAAAGGAGTGGACGTGGGATGAGAGCAAGAT
  GCTGGTGATGCAGGACCCTATCTACAGGATCTTCTATGTCTCTCATGACTCCCAAGACTTGAAAATCTTC
  AGCTATATTGCCAGAGATGGTGCCAGCAATATCTTCAGATGTAACGTCTTTAAATCCAAAAAGAAGAGTC
  AAGCTATGAGAATCGTGCGGACAGTGGGACAGGCCTTCGAAGTCTGCCACAAGCTGAGCCTGCAGCACAC
  ACAGCAGAATGCAGATGGCCAGGAAGATGGGGAGAGCGAGAGGAACAGTGATGGCTCAGGAGACCCAGGC
  CGGCAGCTCACGGGAGCTGAGAGGGTCTCCACAGCCGCTGCAGAGGAGACAGACATTGACGCCGTGGAGG
  TCCCACTCCCTGGGAATGACATTCTGGAATTCAGCCGAGGTGTGACTGATCTGGATGCCGTAGGGAAGGA
  TGGAGGCTCCCACATAGACTCAACGGTCTCACCCCACCCTCAGGAGCCCATGCTGACAGCCTCCCCTCGA
  ATGCTGCTCCCTTCCTCTTCCTCGAAGCCGCCAGGCTTGGGCACGGGGACGCCCCTGTCCACTCACCACC
  AGATGCAGCTCCTCCAGCAGCTCCTCCAGCAGCAGCAACAACAGACACAAGTGGCTGTGGCCCAGGTTCT
  CCTCAGCCTCCTCCCTTCTTCACAGGCTCCACTCTCATGGGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219444 representing NM_027528
  Red=Cloning site Green=Tags(s)
  
  MRRIRYEFKAKNIKKKKVSIMVSVDGVKVILKKKKKKKEWTWDESKMLVMQDPIYRIFYVSHDSQDLKIF
  SYIARDGASNIFRCNVFKSKKKSQAMRIVRTVGQAFEVCHKLSLQHTQQNADGQEDGESERNSDGSGDPG
  RQLTGAERVSTAAAEETDIDAVEVPLPGNDILEFSRGVTDLDAVGKDGGSHIDSTVSPHPQEPMLTASPR
  MLLPSSSSKPPGLGTGTPLSTHHQMQLLQQLLQQQQQQTQVAVAQVLLSLLPSSQAPLSWA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027528
• ORF Size: 813 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027528.2, NP_081804.1
• RefSeq Size: 1533 bp
• RefSeq ORF: 816 bp
• Locus ID: 70729
• Cytogenetics: 1 H3
• Gene Summary: Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4 (By similarity).[UniProtKB/Swiss-Prot Function]