Sgms1 (NM_001168525) Mouse Tagged ORF Clone

CAT#: MG219071

Sgms1 (tGFP-tagged) - Mouse sphingomyelin synthase 1 (Sgms1) transcript variant 1, (10ug)

Product Images

Circular map for MG219071

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sgms1
• Synonyms: 9530058O11Rik; AI841905; C80702; Mob; Sms1; Sor1; Tmem23
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219071 representing NM_001168525
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTCTGCCAGGACCATGAAGGAAGTGGTTTACTGGTCACCCAAGAAGGTGGCAGACTGGCTGCTGG
  AGAATGCTATGCCAGAATACTGTGAGCCTCTGGAGCACTTCACAGGCCAGGACTTAATCAACCTAACCCA
  AGAGGATTTCAAAAAACCCCCACTGTACCGAGTCTCCTCTGACAATGGGCAGCGACTCTTAGACATGATA
  GAGACCCTGAAGATGGAGCACCATATGGAAGCACACAAGAATGGCCACGCCAACGGACACCTCAGCATTG
  GCGTTGACATTCCCAACCCCGATGGCAGCTTCAGCATCAAGACTAAACCCAACGGAATGCCAAATGGGTT
  TAGGAAAGAGATGATCAAGATCCCCATGCCAGAACCGGAGCGCTCCCAGTATCCCATGGAGTGGGGCAAG
  ACTTTCCTGGCCTTTCTTTATGCACTTTCCTGTTTTGTTCTCACTACAGTGATGATCTCGGTCGTCCATG
  AACGAGTACCTCCTAAGGAGGTGCAGCCTCCACTACCGGACACGTTTTTTGACCATTTTAACCGGGTGCA
  GTGGGCGTTTTCTATTTGCGAAATTAACGGCATGATCCTTGTAGGACTCTGGCTATTTCAGTGGCTGCTC
  TTAAAATACAAGTCTATTATTAGCAGAAGATTTTTCTGCATAGTTGGCACGCTGTACCTGTATCGGTGTA
  TTACAATGTATGTAACTACACTCCCAGTACCTGGCATGCATTTCAACTGTTCTCCGAAGCTCTTTGGAGA
  CTGGGAAGCTCAAGTGCGGAGAATAATGAAGCTCATTGCTGGAGGTGGCTTATCCATCACAGGCTCGCAC
  AACATGTGTGGCGACTATCTGTACAGTGGCCACACGGTCATGCTAACGCTCACCTACCTATTTATCAAAG
  AGTATTCTCCTCGGCGGCTCTGGTGGTACCACTGGATTTGCTGGCTCCTCAGCGTCGTTGGAATCTTCTG
  TATTCTCTTAGCGCATGACCACTACACTGTGGACGTGGTGGTGGCCTACTACATCACCACAAGACTCTTC
  TGGTGGTATCACACGATGGCCAATCAGCAAGTGCTTAAGGAAGCCTCCCAGATGAACCTCCTGGCCAGGG
  TGTGGTGGTACAGGCCATTTCAGTACTTTGAAAAGAATGTCCAAGGAATTGTACCTCGATCTTACCATTG
  GCCCTTCCCCTGGCCGGTAGTCCACCTTAGTAGGCAAGTTAAATATAGCCGGCTGGTAAACGACACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219071 representing NM_001168525
  Red=Cloning site Green=Tags(s)
  
  MLSARTMKEVVYWSPKKVADWLLENAMPEYCEPLEHFTGQDLINLTQEDFKKPPLYRVSSDNGQRLLDMI
  ETLKMEHHMEAHKNGHANGHLSIGVDIPNPDGSFSIKTKPNGMPNGFRKEMIKIPMPEPERSQYPMEWGK
  TFLAFLYALSCFVLTTVMISVVHERVPPKEVQPPLPDTFFDHFNRVQWAFSICEINGMILVGLWLFQWLL
  LKYKSIISRRFFCIVGTLYLYRCITMYVTTLPVPGMHFNCSPKLFGDWEAQVRRIMKLIAGGGLSITGSH
  NMCGDYLYSGHTVMLTLTYLFIKEYSPRRLWWYHWICWLLSVVGIFCILLAHDHYTVDVVVAYYITTRLF
  WWYHTMANQQVLKEASQMNLLARVWWYRPFQYFEKNVQGIVPRSYHWPFPWPVVHLSRQVKYSRLVNDT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168525
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168525.1, NP_001161997.1
• RefSeq Size: 4056 bp
• RefSeq ORF: 1260 bp
• Locus ID: 208449
• UniProt ID: Q8VCQ6
• Cytogenetics: 19 C1
• Gene Summary: Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types (By similarity). Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide.[UniProtKB/Swiss-Prot Function]