Cd200r3 (NM_001128132) Mouse Tagged ORF Clone

CAT#: MG218430

Cd200r3 (tGFP-tagged) - Mouse CD200 receptor 3 (Cd200r3) transcript variant 1, (10ug)

Product Images

Circular map for MG218430

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cd200r3
• Synonyms: 4733401I18Rik; 4833409J19Rik; AI505817; BB106877; mCD200RLb
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218430 representing NM_001128132
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGCTTTGGGGAGGACTCTGGCTTTGATGTTACTCATCTTCATCACTATTTTGGTGCCTGAGTCAA
  GTTGTTCAGTGAAAGGACGGGAGGAGATCCCACCGGATGATTCATTTCCTTTTTCAGATGATAATATCTT
  CCCTGATGGAGTGGGCGTCACCATGGAGATTGAGATTATCACTCCAGTGTCTGTACAGATAGGTATCAAG
  GCTCAGCTTTTCTGTCATCCTAGTCCATCAAAAGAAGCAACACTTAGAATATGGGAAATAACTCCCAGAG
  ACTGGCCTTCCTGCAGACTACCCTACAGAGCAGAGTTGCAGCAGATCAGTAAAAAAATCTGTACTGAGAG
  AGGAACCACTAGGGTCCCTGCACATCACCAGAGTTCTGACCTTCCCATCAAATCAATGGCCCTCAAGCAT
  GATGGGCATTACTCATGTCGGATAGAAACAACAGATGGGATTTTCCAAGAGAGACATAGCATCCAAGTGC
  CAGGGGAAAATAGAACTGTAGTTTGTGAGGCAATTGCAAGCAAGCCTGCTATGCAGATCTTGTGGACTCC
  AGATGAGGACTGTGTCACTAAGAGTAAATCACACAATGACACCATGATTGTCAGGAGCAAGTGCCACAGG
  GAGAAAAACAATGGCCACAGTGTGTTCTGCTTTATCTCCCATTTGACTGATAACTGGATTCTCTCCATGG
  AACAGAATCGAGGTACAACCAGCATCCTGCCTTCCTTGCTGAGCATTCTCTATGTGAAACTGGCTGTAAC
  TGTTCTCATCGTAGGATTTGCTTTTTTCCAGAAGAGAAATTATTTCAGAGTGCCAGAAGGCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218430 representing NM_001128132
  Red=Cloning site Green=Tags(s)
  
  MHALGRTLALMLLIFITILVPESSCSVKGREEIPPDDSFPFSDDNIFPDGVGVTMEIEIITPVSVQIGIK
  AQLFCHPSPSKEATLRIWEITPRDWPSCRLPYRAELQQISKKICTERGTTRVPAHHQSSDLPIKSMALKH
  DGHYSCRIETTDGIFQERHSIQVPGENRTVVCEAIASKPAMQILWTPDEDCVTKSKSHNDTMIVRSKCHR
  EKNNGHSVFCFISHLTDNWILSMEQNRGTTSILPSLLSILYVKLAVTVLIVGFAFFQKRNYFRVPEGS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128132
• ORF Size: 834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128132.1, NP_001121604.1
• RefSeq Size: 1622 bp
• RefSeq ORF: 837 bp
• Locus ID: 74603
• UniProt ID: Q5UKY4
• Cytogenetics: 16 B4- B5
• Gene Summary: According to PubMed:15187158 isoform 4 is a receptor for the CD200 cell surface glycoprotein. According to PubMed:16081818 isoform 4 is not a receptor for the CD200/OX2 cell surface glycoprotein. Isoform 1, isoform 2 and isoform 3 are involved in the recruitment or surface expression of the TYROBP receptor. Isoform 6, isoform 7 and isoform 8 are not involved in the recruitment or surface expression of the TYROBP receptor.[UniProtKB/Swiss-Prot Function]