C77080 (NM_001033189) Mouse Tagged ORF Clone

CAT#: MG217648

C77080 (tGFP-tagged) - Mouse expressed sequence C77080 (C77080), (10ug)

Product Images

Circular map for MG217648

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C77080
• Synonyms: Kiaa1522; mKIAA1522
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217648 representing NM_001033189
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCGCGCGCCCCCCGCCGCACCTGCGGCCGACGAGCCTGGGAGCCCCGGCGGCCCCCCGCGCA
  GGAAGAAGTCCCGCTCCGGCCTCCGCCGTGCCTTCAGCTGGCTGAGGGGCAAACGGCGCAAGAAGAAGGC
  GGCCGGGGCCGAGGGCGCCGAGTCCACAGCCTCGCGGGCTAAGAAAGCGGACGACAAGGCCAAGCGGGCC
  AAGGGCAAAAGCCGAGGCTCTGCTAAGGCCGAGAGTGACAACCGTCAGGGCGCAGGGCCCAGCCAGGGGC
  CAGGATCTGTAGGAGATGAACTTCAGGACAACGTTTTCTTCCCCAGTGGGAGACCTCCTCATCTGGAAGA
  GCTGCACACACAGGCCCAGGAGGGGCTCCGCTCCCTCCAGCACCAAGAAAGACAGAAGCTGAGCAAGGGC
  GGCTGGGACCATGGAGACACCCAGAGCATCCAGTCCTCCCAGACGGGGCCGGATGAAGATACCATCTCCA
  TCTACAGCCAGAAGTCATACATGACGGAGAGCTCCACCGCGGAGGATGCGCTCTCCGTCCGCTCGGAGAT
  GATTCAGCGCAGAGGCTCCACCTTCAGACCCCATGACTCATTTCCCAAATCTGGGAAGTCAGGAAGGCGA
  CGGAGGGAGCGAAGGAGCACGGTGCTGGGGCTGCCTCAGCACGTGCAGAAGGAGCTCGGCCTGCGGAACA
  ACCGTGAGGCACCGGGCACTCCACAGCCTCCTGGTTCACGGGACGCTGTCCGCATCCCCACGGTGGATGG
  TCGCCCCGGTCTGGCCTTAGGGACAGGGGTCCGAGTGTCCCTGCAGGCTCTGGAAGCAGAAACAGAGGCC
  GGCACAGATGCAGAGGCTGTCATCCAGCGCCACATCGACCGTGTTTACCACGATGACACGCTTGTTGGCC
  GATCCACGGGAGCCCGGCCACCGCCACTGACGAGGCCAATGTCTCTCGCAGTGCCTGGACTGACAGGAGG
  AGCAGGATCTCCAGAGCCACTGAGCCCAGCCATGTCAATCTCACCCCAGGCCACCTACTTGTCGAAGCTG
  ATCCCGCACGCCGTGCTGCCGCCCACCGTGGATGTGGTGGCCCTGGGCCGCAGCAGCCTGCGCACTCTGA
  GCCGTTGCAGCCTGCTCTCTGCCAGCCCAGCTTCGGTTCGCTCCCTGGGCCGCTTCTCCTCGGCTTCCAG
  TCCACGGCCCCGCAGCCGCAACGCTTCCTCGTCCAGTGACAACTGGAGCCACTCTCAGTCCTCCGAGACC
  ATTGTGTCTGATGGTTCCACTCTTTCCTCTAAGGGGGGCTCTGAGGGCCAGCCAGAAGGCTCTGTAGCTA
  GCAATAATGTGGCGCCCCCTCCTCCAGGCGGTAGTGGGCGGGGCTCCCCCAGCGGGGGTAGCACTGCGGA
  GACCTCAGACACGGCCAGCATCCGAAGCAGTGGGCAGCTGTCTGGCAGGAGTGTGTCCCTGCGTAAGATG
  AAACGGCCTCCCCCGCCTCCCCGCCGGACCTACTCCCTCCATCAGCGCGGCTCCGCAGTGCCTGATGGGC
  CCTTAGGGTTGCCGCCCAAACCTGAGCGAAAGCAGCAGCCACAGCTGCCTCGGCCGCCCACTGCGGGTGG
  CTCTTCAGGGGTGGGGGCAGTATCTTGTCCACCCAGCTCAGCAGGCACCTGGGGCTCTGGCTTGTCCCCA
  GGTGGCTCCAGGCGTCCCCCACGTTCCCCAGAACGGACACTTTCACCTTCAAGTGGCTACTCGAGCCAGA
  GCGGTACCCCAACTCTCCCTCCCAAGGGTCTGGCAGTTGCCCCTGCTTCCCCAGGCAAGGCTCAGCCCCC
  CAAACCAGATCGAGTGACATCCCTTCGATCTCCTGGGGCCTCTGTATCCTCTTCCCTTACATCTCTGTGT
  TCCTCTTCTTCAGACCCTACTCCTTTAGACCGCTCTGGCCCACAAATGTCTACCCCCTTGAGTGACAGGT
  TCGTCATACCTCCTCATCCCAAGGTGCCTGCTCCTTTCTCTCCACCACCTTCCAAGTCCAAGAGCTCGAA
  CCAAGCTGCTCCTGTTCTGGCTGCCCCTGCTGTGGCTCCTGGGCAGGTGTCCACCATCGACACCAGTCCT
  GCATCCCCTTCCATGCCCCAGACAACCTTGACTCCAGCCCAGGAGTCTCCTGTTGCCTCCAAAGATGAGT
  CACCCCCACCATCCCCACCCCCATCTTACCATCCACCCCCACCACCTACTAAGAAGCCGGAGGTGCTGGA
  GGAGGCCCCACCTCCTCCGGAAGCTGCTGTGGAGATCCTTCCAGATCCCAGCTGGCCGCCGCCACCACCA
  CCTGCACCTGAGGAACAGGACCTGTCGATGGCTGACTTCCCCCCTCCTGAGGAGGTCTTCTTCAATGCAG
  GCCCTGAGCTTGGCCCTCTGGAGTCCTGCAGCTCTGAGGCTGCCGTCCCCCCAGCTGCTAGCTTGTCCCA
  GACTCCTCCGCCAGCTCCACCTCCTAGTTCTGGATCAGAACCTCTGGCCAGGCTCCCACAGAAGGACTCA
  GTGGGCAAGCACAGCGGGGCTCCCAGGGAGGATTCGGGCACGCCTCTGGTCACACCCTCGCTCCTGCAGA
  TGGTCCGGCTTCGCTCTGTGGGTGCTTCCACAGGGATTCCGAACCCTTCTCCGGGTTCATCGGCCCCTCA
  GAAGCCTCTGCGAAGAGCCCTGTCTGGGCGCGCCAGCCCAGTGACTGCTCCCTCCTCTGGGCTCCATGCT
  GCCGTCCGACTCAAGGCCTCTAGCCTGGCTGCCAGTGAGAGTCCTGCGAGTGCTCTGCCCACTGGAATAC
  CCGAGGCAGAGCCACGGTCGCCACAGTCTCCTGCCTCCAAGGCCAGCTTCATCTTCTCCAAGGGCACCAA
  AAAACTGCAGCTGGAGAGGCCCGTGTCCCCCGAGGCCCAGGCTGACCTCCAGCGGAATCTGGTGGCTGAA
  CTTCGGAGCATTTCAGAGCATCGGCCACCTCCCCAGGCCCAGAAGAAGCCTTCCAAGGCTCCCCCACCAG
  TGGCCCGCAAACCCTCAGTGGGAGTCCCTCCTCCGTCCCCCAGTCTTCCCAGGACGGAGTCTCTTACTGC
  TCCATCCACCAATGGGCTCCCTCACGCTGAGGACAGGACTAACGGGGAGCTGGCGGAGAATGGAGGTGTG
  CAGCTGGCTGCTACAGAGAAGATGGGCTCCCCTGGTTCAGATCCACAGAAGAAACTGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217648 representing NM_001033189
  Red=Cloning site Green=Tags(s)
  
  MAARAPPAAPAADEPGSPGGPPRRKKSRSGLRRAFSWLRGKRRKKKAAGAEGAESTASRAKKADDKAKRA
  KGKSRGSAKAESDNRQGAGPSQGPGSVGDELQDNVFFPSGRPPHLEELHTQAQEGLRSLQHQERQKLSKG
  GWDHGDTQSIQSSQTGPDEDTISIYSQKSYMTESSTAEDALSVRSEMIQRRGSTFRPHDSFPKSGKSGRR
  RRERRSTVLGLPQHVQKELGLRNNREAPGTPQPPGSRDAVRIPTVDGRPGLALGTGVRVSLQALEAETEA
  GTDAEAVIQRHIDRVYHDDTLVGRSTGARPPPLTRPMSLAVPGLTGGAGSPEPLSPAMSISPQATYLSKL
  IPHAVLPPTVDVVALGRSSLRTLSRCSLLSASPASVRSLGRFSSASSPRPRSRNASSSSDNWSHSQSSET
  IVSDGSTLSSKGGSEGQPEGSVASNNVAPPPPGGSGRGSPSGGSTAETSDTASIRSSGQLSGRSVSLRKM
  KRPPPPPRRTYSLHQRGSAVPDGPLGLPPKPERKQQPQLPRPPTAGGSSGVGAVSCPPSSAGTWGSGLSP
  GGSRRPPRSPERTLSPSSGYSSQSGTPTLPPKGLAVAPASPGKAQPPKPDRVTSLRSPGASVSSSLTSLC
  SSSSDPTPLDRSGPQMSTPLSDRFVIPPHPKVPAPFSPPPSKSKSSNQAAPVLAAPAVAPGQVSTIDTSP
  ASPSMPQTTLTPAQESPVASKDESPPPSPPPSYHPPPPPTKKPEVLEEAPPPPEAAVEILPDPSWPPPPP
  PAPEEQDLSMADFPPPEEVFFNAGPELGPLESCSSEAAVPPAASLSQTPPPAPPPSSGSEPLARLPQKDS
  VGKHSGAPREDSGTPLVTPSLLQMVRLRSVGASTGIPNPSPGSSAPQKPLRRALSGRASPVTAPSSGLHA
  AVRLKASSLAASESPASALPTGIPEAEPRSPQSPASKASFIFSKGTKKLQLERPVSPEAQADLQRNLVAE
  LRSISEHRPPPQAQKKPSKAPPPVARKPSVGVPPPSPSLPRTESLTAPSTNGLPHAEDRTNGELAENGGV
  QLAATEKMGSPGSDPQKKLV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033189
• ORF Size: 3210 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033189.3, NP_001028361.1
• RefSeq Size: 5187 bp
• RefSeq ORF: 3213 bp
• Locus ID: 97130
• UniProt ID: A2A7S8
• Cytogenetics: 4 D2.2