Plcg2 (NM_172285) Mouse Tagged ORF Clone

CAT#: MG211870

Plcg2 (tGFP-tagged) - Mouse phospholipase C, gamma 2 (Plcg2)

Product Images

Circular map for MG211870

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Plcg2
• Synonyms: PLC-gamma-2; Plcg-2; PLCgamma2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211870 representing NM_172285
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACCATGGTCAACGTGGACACCCTTCCAGAATATGAGAAGAGTCAGATCAAGAGAGCGCTGGAGT
  TAGGGACGGTGATGACCGTGTTCAACGCCCGCAAATCCACCCCAGAGCGGAGGACAGTACAGATGATCAT
  GGAGACTCGGCAGGTGGCATGGAGCAAGACCGCAGACAAGATTGAAGGCTTCCTGGACATCATGGAGATA
  AAGGAAATCCGTCCGGGGAAGAACTCCAAGGACTTTGAGCGAGCCAAGGCTGTCCGCCACAAGGCAGAAT
  GTTGCTTCACCATCCTCTACGGCACCCAGTTTGTCCTCAGCACGCTCAGTTTGGCAACGGACTCAAAGGA
  GGATGCGGTGAAGTGGCTCTCTGGTTTGAAGATCCTACACCAGGAAGCGATGAGTGCATCCACCCCCACA
  ATGATTGAGAGTTGGCTGAGAAAACAGATTTATTCAGTAGATCAAACCCGAAGAAACAGCATCAGCCTCC
  GGGAGCTGAAGACCATCTTGCCCCTGGTCAACTTCAAAGTGAGCGGCATCAAGTTTCTCAAGGACAAGCT
  GGTGGAAATCGGCGCACAGAAAGATGAGCTCAGCTTTGAACAGTTCCATCTCTTCTATAAGAAACTCATG
  TTTGACCAGCAAAAATCGATACTTGACGAATTCAAAAAAGACTCCTCCGTGTTCATCCTAGGAAACACAG
  ACCGGCCGGATGCCTCGGCTGTCTACCTGCAGGACTTCCAAAGGTTTCTTTTACATGAACAGCAGGAGCT
  ATGGGCTCAGGATCTGAACAAAGTCCGGGAGCGGATGACCAAGTTTATCGACGACACAATGAGAGAGACC
  GCAGAGCCCTTCTTATTTGTGGATGAGTTCCTCACGTATCTGTTCTCCCGGGAGAACAGCATCTGGGACG
  AGAAGTACGATGCAGTGGACATGCAGGACATGAACAACCCCTTGTCCCACTACTGGATCTCCTCTTCCCA
  CAACACGTACCTCACTGGGGACCAGCTGCGTAGTGAGTCCTCCACGGAAGCGTATATCCGCTGTCTGCGC
  GCTGGTTGCCGCTGCATTGAGCTGGACTGCTGGGACGGGCCTGATGGGAAGCCCATTATCTACCACGGCT
  GGACACGGACCACCAAGATCAAGTTTGATGATGTTGTTCAGGCCATCCGAGACCACGCCTTTGTTACCTC
  CAGCTTCCCCGTCATCTTGTCTATCGAGGAGCACTGCAGTGTGGAGCAGCAACGTCACATGGCCAAGGTG
  TTCAAGGAAGTGTTAGGAGACATGCTGTTGACGAAGCCCACAGAGGCCAGTGCTGACCAGCTGCCCTCGC
  CCAGTCAGCTTCGTGAGAAGATCATTATCAAGCATAAGAAGCTGGGTCCCAAGGGTGATGTCGACGTCAA
  CGTGGAGGACAAGAAAGATGAGCACAAGCCCCAGGGCGAGCTGTATATGTGGGACTCCATCGACCAGAAA
  TGGACGCGCCACTACTGTGCCATTGCTGACGCCAAGCTGTCCTTCGGTGATGACATTGAACAGGCTGTGG
  AGGAGGAGCCGGTCCAGGACACTCCCCCCACGGAGCTGCATTTTGGGGAGAAATGGTTCCACAAGAAGGT
  GGAGAGTAGGACCAGTGCGGAGAAGCTGCTGCAAGAGTACTGTGCTGAGACCGGGGCCAAGGACGGCACC
  TTCCTGGTGCGGGAGAGCGAGACCTTCCCCAATGACTACACACTCTCCTTCTGGCGGTCTGGCCGGGTGC
  AGCACTGCCGGATCCGGTCTACTATGGAGAATGGGGTCATGAAGTACTACCTGACCGACAACCTCACGTT
  CAACAGCATCTACGCCCTCATCCAGCACTACCGTGAGGCACACCTGCGCTGCGCGGAGTTCGAGCTGCGG
  CTCACAGACCCGGTGCCCAACCCCAACCCACACGAGTCCAAGCCGTGGTACTATGACAGTCTGAGCAGGG
  GGGAAGCGGAGGACATGCTGATGCGGATCCCCCGAGATGGAGCCTTCCTCATCCGGAAGAGGGAGGGGAC
  CAACTCCTACGCCATCACCTTCAGGGCCAGGGGCAAGGTGAAACATTGTCGCATCAACCGGGACGGACGC
  CACTTCGTGCTGGGCACCTCTGCCTACTTTGAGAGCCTAGTGGAGCTTGTCAGCTACTACGAGAAGCACG
  CGCTCTATCGCAAGATGAGGCTTCGATACCCCGTGACCCCGGAGCTCCTGGAGCGATATAATATGGAAAG
  AGACATCAACTCTCTTTATGACGTCAGCAGGATGTATGTGGACCCAAGTGAGATCAACCCTTCCATGCCT
  CAGAGGACCGTGAAAGCGCTCTATGACTACAAAGCCAAGCGCAGCGACGAGCTGACCTTCTGCCGCGGTG
  CCCTCATCCACAATGTCTCCAAGGAGCCCGGGGGATGGTGGAAAGGGGACTACGGGACCCGGATCCAGCA
  GTACTTCCCGTCCAACTATGTCGAGGATATCTCGGCAGGCGATGCTGAGGAGATGGAAAAGCAGATTATT
  GAAGACAATCCCCTCGGGTCTCTCTGCAAAGGCATATTGGATCTTAATACCTACAACGTTGTGAAGGCCC
  CCCAGGGGAAAAACCAGAAAGCCTTCGTCTTTATCCTGGAGCCTAAGAAACAGGGAGATCCTCCCGTGGA
  GTTCGCGACCGACCGTGTGGAGGAGCTGTTTGAGTGGTTTCAGAGCATACGGGAGATCACTTGGAAGATG
  GACACCAAGGAGAACAACATGAAGTACTGGGAGAGGAACCAGTCCATCGCCATTGAGCTCTCGGACTTGG
  TTGTATACTGCAAGCCAACTAGCAAAACCAAGGACCATTTGGAAAATCCCGACTTCCGAGAAATCCGCTC
  TTTTGTGGAGACGAAGGCAGACAGCATTGTCAGGCAAAAACCTGTCGATCTATTGCGATACAATCAGAAG
  GGCCTGACCCGAGTCTACCCCAAGGGACAGAGAGTTGACTCTTCGAACTATGACCCCTTCCGTCTGTGGC
  TGTGCGGCTCCCAGATGGTGGCACTCAATTTCCAGACTCCAGACAAATACATGCAGATGAACCACGCGCT
  GTTTTCGCTCAATGGGCGAACAGGCTACGTCCTGCAGCCTGAGAGCATGCGCTCTGAGAAGTATGATCCG
  ATGCCCCTGGAGTCCCAGAGGAAGATCCTGATGACACTCACTGTCAAGGTTCTTGGTGCACGCCACCTCC
  CTAAACTAGGGCGGAGTATTGCCTGTCCTTTTGTGGAGGTGGAAATCTGCGGGGCTGAGTATGACAGCAA
  CAAATTCAAGACGACGGTTGTGAATGACAATGGCCTCAGCCCTGTCTGGGCTCCAACTCAGGAGAAGGTG
  ACATTTGAGATCTATGATCCAAACCTTGCCTTCCTACGCTTTGTGGTCTACGAAGAAGACATGTTCAGTG
  ATCCCAACTTCCTGGCTCATGCCACGTACCCCATTAAAGGCATCAAATCCGGGTTTAGATCAGTCCCTCT
  GAAGAATGGGTACAGTGAAGACATCGAGCTGGCATCCCTCCTGGTTTTCTGTGAGATGAGGCCAGTCCTG
  GAGAGTGAAGAGGAACTCTATTCCTCCTGTCGCCAACTGCGGAGGCGGCAAGAAGAGCTCAACAACCAGC
  TCTTCCTTTACGACACGCACCAGAACCTGCGCGGAGCCAACCGTGATGCCCTGGTGAAGGAGTTCAATGT
  TAACGAGAATCAGCTGCAGCTGTACCAGGAGAAGTGCAACCGGAGGCTGAGAGAGAAGAGAGTAAGCAAC
  AGCAGGTTCTACTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211870 representing NM_172285
  Red=Cloning site Green=Tags(s)
  
  MTTMVNVDTLPEYEKSQIKRALELGTVMTVFNARKSTPERRTVQMIMETRQVAWSKTADKIEGFLDIMEI
  KEIRPGKNSKDFERAKAVRHKAECCFTILYGTQFVLSTLSLATDSKEDAVKWLSGLKILHQEAMSASTPT
  MIESWLRKQIYSVDQTRRNSISLRELKTILPLVNFKVSGIKFLKDKLVEIGAQKDELSFEQFHLFYKKLM
  FDQQKSILDEFKKDSSVFILGNTDRPDASAVYLQDFQRFLLHEQQELWAQDLNKVRERMTKFIDDTMRET
  AEPFLFVDEFLTYLFSRENSIWDEKYDAVDMQDMNNPLSHYWISSSHNTYLTGDQLRSESSTEAYIRCLR
  AGCRCIELDCWDGPDGKPIIYHGWTRTTKIKFDDVVQAIRDHAFVTSSFPVILSIEEHCSVEQQRHMAKV
  FKEVLGDMLLTKPTEASADQLPSPSQLREKIIIKHKKLGPKGDVDVNVEDKKDEHKPQGELYMWDSIDQK
  WTRHYCAIADAKLSFGDDIEQAVEEEPVQDTPPTELHFGEKWFHKKVESRTSAEKLLQEYCAETGAKDGT
  FLVRESETFPNDYTLSFWRSGRVQHCRIRSTMENGVMKYYLTDNLTFNSIYALIQHYREAHLRCAEFELR
  LTDPVPNPNPHESKPWYYDSLSRGEAEDMLMRIPRDGAFLIRKREGTNSYAITFRARGKVKHCRINRDGR
  HFVLGTSAYFESLVELVSYYEKHALYRKMRLRYPVTPELLERYNMERDINSLYDVSRMYVDPSEINPSMP
  QRTVKALYDYKAKRSDELTFCRGALIHNVSKEPGGWWKGDYGTRIQQYFPSNYVEDISAGDAEEMEKQII
  EDNPLGSLCKGILDLNTYNVVKAPQGKNQKAFVFILEPKKQGDPPVEFATDRVEELFEWFQSIREITWKM
  DTKENNMKYWERNQSIAIELSDLVVYCKPTSKTKDHLENPDFREIRSFVETKADSIVRQKPVDLLRYNQK
  GLTRVYPKGQRVDSSNYDPFRLWLCGSQMVALNFQTPDKYMQMNHALFSLNGRTGYVLQPESMRSEKYDP
  MPLESQRKILMTLTVKVLGARHLPKLGRSIACPFVEVEICGAEYDSNKFKTTVVNDNGLSPVWAPTQEKV
  TFEIYDPNLAFLRFVVYEEDMFSDPNFLAHATYPIKGIKSGFRSVPLKNGYSEDIELASLLVFCEMRPVL
  ESEEELYSSCRQLRRRQEELNNQLFLYDTHQNLRGANRDALVKEFNVNENQLQLYQEKCNRRLREKRVSN
  SRFYS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172285
• ORF Size: 3795 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172285.1, NP_758489.1
• RefSeq Size: 4345 bp
• RefSeq ORF: 3798 bp
• Locus ID: 234779
• UniProt ID: Q8CIH5
• Cytogenetics: 8 64.26 cM
• Gene Summary: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling (By similarity).[UniProtKB/Swiss-Prot Function]