Plcg2 (NM_172285) Mouse Tagged ORF Clone

USD 1,330.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Plcg2 (NM_172285) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Plcg2
Synonyms PLC-gamma-2; Plcg-2; PLCgamma2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR211870 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCACCATGGTCAACGTGGACACCCTTCCAGAATATGAGAAGAGTCAGATCAAGAGAGCGCTGGAGT
TAGGGACGGTGATGACCGTGTTCAACGCCCGCAAATCCACCCCAGAGCGGAGGACAGTACAGATGATCAT
GGAGACTCGGCAGGTGGCATGGAGCAAGACCGCAGACAAGATTGAAGGCTTCCTGGACATCATGGAGATA
AAGGAAATCCGTCCGGGGAAGAACTCCAAGGACTTTGAGCGAGCCAAGGCTGTCCGCCACAAGGCAGAAT
GTTGCTTCACCATCCTCTACGGCACCCAGTTTGTCCTCAGCACGCTCAGTTTGGCAACGGACTCAAAGGA
GGATGCGGTGAAGTGGCTCTCTGGTTTGAAGATCCTACACCAGGAAGCGATGAGTGCATCCACCCCCACA
ATGATTGAGAGTTGGCTGAGAAAACAGATTTATTCAGTAGATCAAACCCGAAGAAACAGCATCAGCCTCC
GGGAGCTGAAGACCATCTTGCCCCTGGTCAACTTCAAAGTGAGCGGCATCAAGTTTCTCAAGGACAAGCT
GGTGGAAATCGGCGCACAGAAAGATGAGCTCAGCTTTGAACAGTTCCATCTCTTCTATAAGAAACTCATG
TTTGACCAGCAAAAATCGATACTTGACGAATTCAAAAAAGACTCCTCCGTGTTCATCCTAGGAAACACAG
ACCGGCCGGATGCCTCGGCTGTCTACCTGCAGGACTTCCAAAGGTTTCTTTTACATGAACAGCAGGAGCT
ATGGGCTCAGGATCTGAACAAAGTCCGGGAGCGGATGACCAAGTTTATCGACGACACAATGAGAGAGACC
GCAGAGCCCTTCTTATTTGTGGATGAGTTCCTCACGTATCTGTTCTCCCGGGAGAACAGCATCTGGGACG
AGAAGTACGATGCAGTGGACATGCAGGACATGAACAACCCCTTGTCCCACTACTGGATCTCCTCTTCCCA
CAACACGTACCTCACTGGGGACCAGCTGCGTAGTGAGTCCTCCACGGAAGCGTATATCCGCTGTCTGCGC
GCTGGTTGCCGCTGCATTGAGCTGGACTGCTGGGACGGGCCTGATGGGAAGCCCATTATCTACCACGGCT
GGACACGGACCACCAAGATCAAGTTTGATGATGTTGTTCAGGCCATCCGAGACCACGCCTTTGTTACCTC
CAGCTTCCCCGTCATCTTGTCTATCGAGGAGCACTGCAGTGTGGAGCAGCAACGTCACATGGCCAAGGTG
TTCAAGGAAGTGTTAGGAGACATGCTGTTGACGAAGCCCACAGAGGCCAGTGCTGACCAGCTGCCCTCGC
CCAGTCAGCTTCGTGAGAAGATCATTATCAAGCATAAGAAGCTGGGTCCCAAGGGTGATGTCGACGTCAA
CGTGGAGGACAAGAAAGATGAGCACAAGCCCCAGGGCGAGCTGTATATGTGGGACTCCATCGACCAGAAA
TGGACGCGCCACTACTGTGCCATTGCTGACGCCAAGCTGTCCTTCGGTGATGACATTGAACAGGCTGTGG
AGGAGGAGCCGGTCCAGGACACTCCCCCCACGGAGCTGCATTTTGGGGAGAAATGGTTCCACAAGAAGGT
GGAGAGTAGGACCAGTGCGGAGAAGCTGCTGCAAGAGTACTGTGCTGAGACCGGGGCCAAGGACGGCACC
TTCCTGGTGCGGGAGAGCGAGACCTTCCCCAATGACTACACACTCTCCTTCTGGCGGTCTGGCCGGGTGC
AGCACTGCCGGATCCGGTCTACTATGGAGAATGGGGTCATGAAGTACTACCTGACCGACAACCTCACGTT
CAACAGCATCTACGCCCTCATCCAGCACTACCGTGAGGCACACCTGCGCTGCGCGGAGTTCGAGCTGCGG
CTCACAGACCCGGTGCCCAACCCCAACCCACACGAGTCCAAGCCGTGGTACTATGACAGTCTGAGCAGGG
GGGAAGCGGAGGACATGCTGATGCGGATCCCCCGAGATGGAGCCTTCCTCATCCGGAAGAGGGAGGGGAC
CAACTCCTACGCCATCACCTTCAGGGCCAGGGGCAAGGTGAAACATTGTCGCATCAACCGGGACGGACGC
CACTTCGTGCTGGGCACCTCTGCCTACTTTGAGAGCCTAGTGGAGCTTGTCAGCTACTACGAGAAGCACG
CGCTCTATCGCAAGATGAGGCTTCGATACCCCGTGACCCCGGAGCTCCTGGAGCGATATAATATGGAAAG
AGACATCAACTCTCTTTATGACGTCAGCAGGATGTATGTGGACCCAAGTGAGATCAACCCTTCCATGCCT
CAGAGGACCGTGAAAGCGCTCTATGACTACAAAGCCAAGCGCAGCGACGAGCTGACCTTCTGCCGCGGTG
CCCTCATCCACAATGTCTCCAAGGAGCCCGGGGGATGGTGGAAAGGGGACTACGGGACCCGGATCCAGCA
GTACTTCCCGTCCAACTATGTCGAGGATATCTCGGCAGGCGATGCTGAGGAGATGGAAAAGCAGATTATT
GAAGACAATCCCCTCGGGTCTCTCTGCAAAGGCATATTGGATCTTAATACCTACAACGTTGTGAAGGCCC
CCCAGGGGAAAAACCAGAAAGCCTTCGTCTTTATCCTGGAGCCTAAGAAACAGGGAGATCCTCCCGTGGA
GTTCGCGACCGACCGTGTGGAGGAGCTGTTTGAGTGGTTTCAGAGCATACGGGAGATCACTTGGAAGATG
GACACCAAGGAGAACAACATGAAGTACTGGGAGAGGAACCAGTCCATCGCCATTGAGCTCTCGGACTTGG
TTGTATACTGCAAGCCAACTAGCAAAACCAAGGACCATTTGGAAAATCCCGACTTCCGAGAAATCCGCTC
TTTTGTGGAGACGAAGGCAGACAGCATTGTCAGGCAAAAACCTGTCGATCTATTGCGATACAATCAGAAG
GGCCTGACCCGAGTCTACCCCAAGGGACAGAGAGTTGACTCTTCGAACTATGACCCCTTCCGTCTGTGGC
TGTGCGGCTCCCAGATGGTGGCACTCAATTTCCAGACTCCAGACAAATACATGCAGATGAACCACGCGCT
GTTTTCGCTCAATGGGCGAACAGGCTACGTCCTGCAGCCTGAGAGCATGCGCTCTGAGAAGTATGATCCG
ATGCCCCTGGAGTCCCAGAGGAAGATCCTGATGACACTCACTGTCAAGGTTCTTGGTGCACGCCACCTCC
CTAAACTAGGGCGGAGTATTGCCTGTCCTTTTGTGGAGGTGGAAATCTGCGGGGCTGAGTATGACAGCAA
CAAATTCAAGACGACGGTTGTGAATGACAATGGCCTCAGCCCTGTCTGGGCTCCAACTCAGGAGAAGGTG
ACATTTGAGATCTATGATCCAAACCTTGCCTTCCTACGCTTTGTGGTCTACGAAGAAGACATGTTCAGTG
ATCCCAACTTCCTGGCTCATGCCACGTACCCCATTAAAGGCATCAAATCCGGGTTTAGATCAGTCCCTCT
GAAGAATGGGTACAGTGAAGACATCGAGCTGGCATCCCTCCTGGTTTTCTGTGAGATGAGGCCAGTCCTG
GAGAGTGAAGAGGAACTCTATTCCTCCTGTCGCCAACTGCGGAGGCGGCAAGAAGAGCTCAACAACCAGC
TCTTCCTTTACGACACGCACCAGAACCTGCGCGGAGCCAACCGTGATGCCCTGGTGAAGGAGTTCAATGT
TAACGAGAATCAGCTGCAGCTGTACCAGGAGAAGTGCAACCGGAGGCTGAGAGAGAAGAGAGTAAGCAAC
AGCAGGTTCTACTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211870 protein sequence
Red=Cloning site Green=Tags(s)

MTTMVNVDTLPEYEKSQIKRALELGTVMTVFNARKSTPERRTVQMIMETRQVAWSKTADKIEGFLDIMEI
KEIRPGKNSKDFERAKAVRHKAECCFTILYGTQFVLSTLSLATDSKEDAVKWLSGLKILHQEAMSASTPT
MIESWLRKQIYSVDQTRRNSISLRELKTILPLVNFKVSGIKFLKDKLVEIGAQKDELSFEQFHLFYKKLM
FDQQKSILDEFKKDSSVFILGNTDRPDASAVYLQDFQRFLLHEQQELWAQDLNKVRERMTKFIDDTMRET
AEPFLFVDEFLTYLFSRENSIWDEKYDAVDMQDMNNPLSHYWISSSHNTYLTGDQLRSESSTEAYIRCLR
AGCRCIELDCWDGPDGKPIIYHGWTRTTKIKFDDVVQAIRDHAFVTSSFPVILSIEEHCSVEQQRHMAKV
FKEVLGDMLLTKPTEASADQLPSPSQLREKIIIKHKKLGPKGDVDVNVEDKKDEHKPQGELYMWDSIDQK
WTRHYCAIADAKLSFGDDIEQAVEEEPVQDTPPTELHFGEKWFHKKVESRTSAEKLLQEYCAETGAKDGT
FLVRESETFPNDYTLSFWRSGRVQHCRIRSTMENGVMKYYLTDNLTFNSIYALIQHYREAHLRCAEFELR
LTDPVPNPNPHESKPWYYDSLSRGEAEDMLMRIPRDGAFLIRKREGTNSYAITFRARGKVKHCRINRDGR
HFVLGTSAYFESLVELVSYYEKHALYRKMRLRYPVTPELLERYNMERDINSLYDVSRMYVDPSEINPSMP
QRTVKALYDYKAKRSDELTFCRGALIHNVSKEPGGWWKGDYGTRIQQYFPSNYVEDISAGDAEEMEKQII
EDNPLGSLCKGILDLNTYNVVKAPQGKNQKAFVFILEPKKQGDPPVEFATDRVEELFEWFQSIREITWKM
DTKENNMKYWERNQSIAIELSDLVVYCKPTSKTKDHLENPDFREIRSFVETKADSIVRQKPVDLLRYNQK
GLTRVYPKGQRVDSSNYDPFRLWLCGSQMVALNFQTPDKYMQMNHALFSLNGRTGYVLQPESMRSEKYDP
MPLESQRKILMTLTVKVLGARHLPKLGRSIACPFVEVEICGAEYDSNKFKTTVVNDNGLSPVWAPTQEKV
TFEIYDPNLAFLRFVVYEEDMFSDPNFLAHATYPIKGIKSGFRSVPLKNGYSEDIELASLLVFCEMRPVL
ESEEELYSSCRQLRRRQEELNNQLFLYDTHQNLRGANRDALVKEFNVNENQLQLYQEKCNRRLREKRVSN
SRFYS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_172285
ORF Size 3798 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_172285.1, NM_172285.2, NP_758489.1
RefSeq Size 4345 bp
RefSeq ORF 3798 bp
Locus ID 234779
Cytogenetics 8 64.26 cM
MW 147.6 kDa

Citations (1)

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