Fnip1 (NM_173753) Mouse Tagged ORF Clone

CAT#: MG211733

Fnip1 (tGFP-tagged) - Mouse folliculin interacting protein 1 (Fnip1)

Product Images

Circular map for MG211733

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fnip1
• Synonyms: A730024A03Rik; AI838773; AW557298
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211733 representing NM_173753
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCACCCTGTTCCAGAAGCTCTTCAGCAAGCGGAGCGGATTAGGCGCGCCGGGACGAGACGCCC
  GCGACCCGGACTGCGCGTTCAGTTGGCCCTTGCCAGAATTTGACCCAAGTCAGATTCGACTGATCGTATA
  TCAAGACTGTGAAAGACGAGGGAGAAATGTATTGTTTGACTCCAGTGTTAAGAGAAAAAATGAGGACACA
  TCAGTATCGAAGCTCTGTAATGATGCTCAAGTCAAGGTCTTTGGGAAGTGCTGCCAGCTGAAGCCAGGAG
  GAGATAGTTCTTCCTCTTTGGATAGCTCTATCACTTTATCTTCTGATGGAAAAGACCAGTGTCCCAAATA
  CCAGGGTTCTCGGTGTTCTTCTGATGCCAATATGCTTGGAGAGATGATGTTTGGCTCAGTAGCAATGAGC
  TACAAAGGATCCACCTTAAAAATTCATCAGATCCGCTCGCCTCCACAGCTTATGCTTAGCAAAGTTTTCA
  CCGCACGGACTGGCAGTAGTATCTGTGGGAGTCTCAATACTCTGCAGGACAGTCTTGAATTCATCAATCA
  GGACAGTAATACATTGAAAGCTGATAGCAGCACAGTTAGTAATGGGCTGCTTGGAAATATAGGTCTTTCA
  CAGTTCTGCAGCCCCAGGCGGGCTTTCTCTGAGCAGGGTCCGCTCCGCCTGATCAGGAGTGCCTCTTTCT
  TTGCAGTTCACAGCAACCCAATGGACATGCCAGGAAGAGAACTGAATGAGGACAGAGACAGCGGCATAGC
  ACGTTCTGCATCTCTGAGCAGCTTGTTCATCACCCCATTCCCCTCCCCAAACTCTTCACTTACCCGAAGC
  TGTGCTAGCAGCTACCAGCGACGTTGGCGACGTAGCCAAACAACAAGTTTGGAAAATGGGGTGTTTCCTA
  GATGGTCTGTAGAAGAAAGCTTTAATCTGTCAGATGAAAGTTGTGGCCCTAATCCAGGAATTGTGAGGAA
  AAAGAAGATTGCAATTGGGGTAATCTTTTCATTATCCAAAGATGAAGATGAAAATAACAAATTCAATGAA
  TTCTTTTTTTCACATTTTCCTCTCTTTGAAAGCCACATGAACAAATTAAAAAGTGCAATAGAACAGGCAA
  TGAAAATGAGCCGGAGATCAGCTGATGCCAGCCAGAGAAGTTTGGCATACAATCGAATACTTGATGCCCT
  GACTGAGTTCAGAACAACAATTTGTAATCTTTATACAATGCCAAGAATTGGGGAACCTGTCTGGCTTACA
  ATGATGTCAGGGACTCCAGAGAAGAACCAGCTTTGCCATCGTTTCATGAAGGAATTCACCTTTCTAATGG
  AAAATGCCTCCAAAAATCAATTCTTGCCAGCTCTCATTACTGCAGTTTTGACCAATCATCTTGCCTGGGT
  TCCAACAGTCATGCCAAATGGACAGCCACCTATAAAAATATTTTTAGAAAAACATTCCTCTCAGAGTGTG
  GGCATGTTGGCAAAGACTCATCCATATAACCCTCTTTGGGCACAACTTGGAGATTTGTATGGCGCTATTG
  GATCTCCTGTACGGTTAGCAAGAACTGTAGTCGTTGGCAAACGACAAGACCTGGTCCAGAGACTGCTTTA
  TTTTCTTACTTACTTCATCAGATGCTCTGAACTTCAAGAAACCCATCTTTTAGAAAATGGAGAAGATGAA
  GCCATTGTTATGCCAGGCACAGTGATTACTACCACTTTAGAAAAGGGGGAAATAGAGGAATCAGAGTATG
  TGCTAATCACAATGCATAGAAACAAAAGCAGCTTGCTCTTTAAAGAGTCAGAAGAGACAAGAACTCCCAA
  CTGTAACTGTAAATATTGCAGTCATCCAGTCCTTGGGCAAAATACAGAGAATGTGTCACAACCAGAAAGA
  GAAGATACTCAAGACAACTCTAAGGAATTGCTAGGAATTTCAGATGAATGCCAAAAGATTTCTCCTCCTG
  ACTGCCAAGAAGAAAATGCTGTTGATGTTCAACAGTACAGAGATAAATTAAGAACTTGCCTTGACACCAA
  GTTAGAGACAGTTGTTTGTACAGGATCTGCTCCAGCCGACAAATGTGTGTTGTCAGAGACATGCTTGGAA
  CCAAGAGAAGAATCCTGGCAGAATAAGGAATTGCTGGATTCAGATAATCACACAGGCACAGCAATGAGAC
  CCACAGGAATAGTTGTTGAAAAGAAACCTCCAGATAAGAATGTGCCCAGTGCATTTTCCTGTGAGGTAAC
  TCAGACAAAGGTTACGTTCTTGATTGGAGATTCTATGTCACCTGATTCAGATACTGAACTTCGGAGTCAG
  GCTGTGGTGGATCAGATTAACAGACATCACAGCGAACCACTAAAGGAAGACAGAGGAGTGGCTGATAAGC
  ATCAAGAAAGTAAAATAACTAAGGACCAATCTGAAGACTCTGACACACAGAACATAGTTTCTGGAGAGTC
  CTGTGAGCTTCCTTGTTGGAGTCATTCAGATCCAGAAAGCATGAGCTTGTTTGATGAATATTTTAATGAT
  GATTCAATTGAAACCAGGACTATTGATGATGTTCCAGTTAAAACAAGTACAGATAGTAAAGAGTATTGCT
  GTATGTTGGAGTATCCAAAAAGATTGTATACAAAAACTAACAAACAGAAAAGTGAACTTTGTAAATGTAT
  AGAAACAGTTCATCAAGATTCATGTAATGCCTGCTTTCCTCAGCAGGACCAGAGGAATTCCCTCTCCATT
  CTTGTCCCCCATGGGGATAAAGAGAGTTCAGACAAAAAAAATGCTGTAGGAACTGAATGGGACATTCCAA
  GAAATGAAAGTTCAGATAGTGCCCTTGGAGATAGTGAGAGTGAAGACACAGGCCCGGACATAAGGAGACA
  AGCTGGCGGTTACTGTGGAGGGGACCAGGAAGACTGGACAGAAGAGGATGAGATACCTTTTCCTGGGTCA
  AAATTGATTGAAGTGAGTGCTGTTCAGCCCAACATTGCCAACTTTGGAAGATCTTTGCTGGGTGGCTACT
  GCTCATCTTATGTTCCTGACTTTGTTCTTCAAGGAATTGGAAATGATGAGAGGCTCCGTCAGTGCCTGGT
  ATCAGATTTGTCTCATGCTGTGCAGCATCCAGTGTTAGATGAACCAATAGCAGAAGCTGTCTGTATTATA
  GCTGATATGGATAAATGGACTGTTCAAGTGGCCAGCAGTCAGAGACGAGTAACAGATAATAAATTGGGAA
  AGGAGGTGTTGGTCTCTAGTCTTGTTTCCAACCTGCTTCATTCCACTCTTCAGCTTTATAAACATAATTT
  ATCTCCAAACTTTTGTGTAATGCACCTTGAAGACCGGTTACAGGAGTTATACTTCAAGAGCAAAATGCTG
  TCTGAGTACCTGAGGGGACAGATGCGTGTTCATGTCAAGGAGCTAGGAGTGGTTCTTGGGATTGAATCCA
  GTGACCTTCCTCTTCTGGCTGCGGTAGCAAGCACCCACTCTCCATACGTTGCTCAGATACTCCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211733 representing NM_173753
  Red=Cloning site Green=Tags(s)
  
  MAPTLFQKLFSKRSGLGAPGRDARDPDCAFSWPLPEFDPSQIRLIVYQDCERRGRNVLFDSSVKRKNEDT
  SVSKLCNDAQVKVFGKCCQLKPGGDSSSSLDSSITLSSDGKDQCPKYQGSRCSSDANMLGEMMFGSVAMS
  YKGSTLKIHQIRSPPQLMLSKVFTARTGSSICGSLNTLQDSLEFINQDSNTLKADSSTVSNGLLGNIGLS
  QFCSPRRAFSEQGPLRLIRSASFFAVHSNPMDMPGRELNEDRDSGIARSASLSSLFITPFPSPNSSLTRS
  CASSYQRRWRRSQTTSLENGVFPRWSVEESFNLSDESCGPNPGIVRKKKIAIGVIFSLSKDEDENNKFNE
  FFFSHFPLFESHMNKLKSAIEQAMKMSRRSADASQRSLAYNRILDALTEFRTTICNLYTMPRIGEPVWLT
  MMSGTPEKNQLCHRFMKEFTFLMENASKNQFLPALITAVLTNHLAWVPTVMPNGQPPIKIFLEKHSSQSV
  GMLAKTHPYNPLWAQLGDLYGAIGSPVRLARTVVVGKRQDLVQRLLYFLTYFIRCSELQETHLLENGEDE
  AIVMPGTVITTTLEKGEIEESEYVLITMHRNKSSLLFKESEETRTPNCNCKYCSHPVLGQNTENVSQPER
  EDTQDNSKELLGISDECQKISPPDCQEENAVDVQQYRDKLRTCLDTKLETVVCTGSAPADKCVLSETCLE
  PREESWQNKELLDSDNHTGTAMRPTGIVVEKKPPDKNVPSAFSCEVTQTKVTFLIGDSMSPDSDTELRSQ
  AVVDQINRHHSEPLKEDRGVADKHQESKITKDQSEDSDTQNIVSGESCELPCWSHSDPESMSLFDEYFND
  DSIETRTIDDVPVKTSTDSKEYCCMLEYPKRLYTKTNKQKSELCKCIETVHQDSCNACFPQQDQRNSLSI
  LVPHGDKESSDKKNAVGTEWDIPRNESSDSALGDSESEDTGPDIRRQAGGYCGGDQEDWTEEDEIPFPGS
  KLIEVSAVQPNIANFGRSLLGGYCSSYVPDFVLQGIGNDERLRQCLVSDLSHAVQHPVLDEPIAEAVCII
  ADMDKWTVQVASSQRRVTDNKLGKEVLVSSLVSNLLHSTLQLYKHNLSPNFCVMHLEDRLQELYFKSKML
  SEYLRGQMRVHVKELGVVLGIESSDLPLLAAVASTHSPYVAQILL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173753
• ORF Size: 3495 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173753.4, NP_776114.2
• RefSeq Size: 6281 bp
• RefSeq ORF: 3498 bp
• Locus ID: 216742
• UniProt ID: Q68FD7
• Cytogenetics: 11 B1.3
• Gene Summary: Acts as a co-chaperone of HSP90AA1. Inhibits the ATPase activity of HSP90AA1 leading to reduction in its chaperone activity. Facilitates the binding of client protein FLCN to HSP90AA1. Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.[UniProtKB/Swiss-Prot Function]