Rbl2 (NM_011250) Mouse Tagged ORF Clone

CAT#: MG211690

Rbl2 (tGFP-tagged) - Mouse retinoblastoma-like 2 (Rbl2)

Product Images

Circular map for MG211690

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rbl2
• Synonyms: p130; PRB2; Rb2; RBR-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211690 representing NM_011250
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCTGGAGGCAACCAGTCGCCACCGCCTCCTCCAGCTGCTGCAGCCAGCTCGGAGGAAGAGGAGG
  AGGATGGCGACGCCGCGGATCGCGCGCAGCCCGCGGGGTCCCCGAGCCATCAGATCCAGCAGCGGTTCGA
  GGAGCTGTGCAGCCGCCTCAACATGGACGAGGCGGCGCGCGCCGAGGCCTGGAGCAGCTACCGCAGCATG
  AGCGAGAGCTACACGCTGGAGGGAAATGACCTTCATTGGTTAGCATGTGCCTTATATGTGGCTTGCAGAA
  AATCTGTTCCAACTGTGAGCAAAGGGACCGCTGAAGGAAACTATGTATCTTTAACCAGAATCCTTCGCTG
  TTCGGAGCAGAGCCTAATTGAATTTTTTAACAAGATGAAGAAGTGGGAAGACATGGCAAATCTGCCCCCA
  CATTTCCGAGAACGTACTGAAAGATTAGAAAGAAACTTCACTGTTTCTGCTGTGATTTTTAAGAAATATG
  AACCCATTTTTCAAGACATTTTTAAATATCCCCAAGAAGAACAGCCTCGCCAGCAAAGAGGAAGAAAACA
  GAGGCGACAGCCCTGTACCACATCAGAAATTTTCCATTTTTGCTGGGTGCTTTTTATATATGCGAAAGGG
  AACTTCCCCATGATTAGCGATGATCTGGTCAATTCCTACCATCTTCTGCTGTGCGCATTAGATTTAGTCT
  ATGGAAATGCCCTTCAGTGTTCTAACCGTAAAGAACTTGTGAACCCTAATTTTAAAGGCCTGTCCGAGGA
  CTGTCACCCCAAGGACTCTAAAGCGTCCTCCGACCCGCCGTGTGTCATTGAGAAGCTGTGCTCCTTACAC
  GACGGTCTAGTGTTGGAGGCCAAGGGGATAAAGGAACACTTCTGGAAACCCTATATTAGGAAACTGTTTG
  AGAAAAAGCTTCTCAAGGGGAAGGAAGAAAATCTTACTGGCTTCCTGGAGCCCGGAAACTTTGGAGAGAG
  TTTTAAGGCCGTTAATAAGGCATATGAAGAATACGTGTTAGCCGCTGGGAATCTGGATGAGCGCGTATTC
  CTTGGTGAGGATGCTGAGGAGGAAGTTGGGACTCTGTCTCGGTGTCTAAGTGCTGCCTCAGGTACAGAGA
  GTGCTGAACGGACGCAGATGAGAGACATCTTGCAGCAGCATCTTGACAAGTCTAAAGCACTTAGAGTCTG
  CACACCACTGACTGGCGTGAGGTATGTTCAGGAGAACAGCCCGTGTGTGACTCCCGTCTCCACAGCTGCA
  CACAGCCTGAGCCGTCTTCACACCATGCTGTCCGGCCTCAGGAATGCACCCAGTGAGAAGCTGGAGCGGA
  TACTCAGGTCATGTTCCCGAGATCCAACTCAGGCTATCGCTGACAGATTGAAAGAAATGTACGAAATATA
  TTCTCAGCATTTCCAGCCAGATGAGAATTTTAGTAATTGTGCTAAAGAAATTGCCAACAAACATTTTCGT
  TTTGCAGAAATGCTCTACTATAAAGTATTAGAGTCTGTTATTGAGCAAGAACAAAAAAGATTGGGAGACA
  TGGATTTATCTGGTGTTCTGGAGCATGACGCATTCCACAGGTCACTCTTGGCCTGCTGCCTTGAGGTGGT
  CGCTTTTTCCCATAAGCCTCCTGGGAATTTTCCATTTATTGCTGAAATATTTGATGTACCACATTATCAT
  TTTTATAAGGTAATTGAAGTATTTATTAGAGCAGAAGACGGTCTTTGCAGAGAAGTGGTCAAACACCTCA
  ATCAGATTGAAGAACAAATTTTAGACCATTTGGCATGGAAAACCAAGTCCCCACTGTGGGACAGAATTAG
  AGATAATGAAAACAGAGTCCCTACTTGTGAAGAGGTCATGCCACCTCAAAACCTAGAGAGAACAGATGAA
  ATTTACATCGCTGGCTCTCCCTTAACCCCGAGAAGGGTGGGTGAAGTTCGTGCTGATGCTGGAGGACTTG
  GAAGAAGTATAACGTCTCCAACCACATTGTATGACAGGTACAGCTCCCCAACAGTCAGCACTACTAGAAG
  GCGGCTATTCGAGAATGATAGTCCCTCTGAAGGAAGCACATCTGGGCGCATCCCCCCACAACCCCTAGTC
  AACGCTGTCCCCGTGCAGAATGTACCTGGGGAGACTGTTTCTGTCACACCAGTTCCTGGACAGACCTTGG
  TCACCATGGCAACAGCCACTGTCACGGCCAACAATGGACAAACAGTGACCATTCCAGTCCAAGGTATTGC
  CAACGAAAATGGAGGGATAACCTTCTTCCCAGTCCAAGTCAACGTTGGGGGCCAGGCCCAGGCTGTCGCT
  GGCTCTATCCAGCCCCTCAGTGCTCAAGCACTGGCTGGAAGTCTGAGTTCCCAACAGGTGACAGGAACCA
  CTTTGCAAGTCCCTGGTCCGGTGGCCATTCAACAGATTTCCCCTGGTGGACAACAGCAGAACCCAGGCCA
  GCCACTAACCAGCAGCAGTATCCGGCCGCGGAAGACTAGCTCCTTAGCGCTCTTCTTTAGAAAGGTTTAC
  TACTTAGCCGGTGTCCGCCTTCGAGATCTTTGTATAAAACTAGATATTTCAGATGAACTGAGGAAAAAAA
  TTTGGACCTGCTTTGAATTCTCTATAATCCAGTGCACCGAACTTATGATGGACAGACATCTGGACCAGCT
  GTTGATGTGTGCCATTTATGTGATGGCAAAGGTCACAAAAGAAGACAGGTCCTTCCAGAACATCATGCGT
  TGTTACAGAACTCAGCCACAGGCCCGGAGCCAGGTGTACAGAAGTGTCTTGATAAAAGGGAAAAGAAGAA
  ACTCTGGCAGCAGTGAGAGCAGAAGCCATCAGAATTCTCCAACCGAACTAAATACAGACAGAGCCAGTAG
  AGATTCCAGCCCAGTGATGAGGTCAAACAGCACCCTACCAGTTCCACAGCCCAGCAGTGCCCCTCCTACA
  CCAACTCGACTCACGGGTGCCAGCAGTGACGTTGAAGAGGAGGAACGAGGAGACCTCATTCAGTTCTACA
  ACAACATCTATAGGAAGCAAATCCAAGCGTTTGCCATGAAGTACTCGCAGGCAAACGCGCAGACGGACAC
  TCCTCCCCTCTCTCCCTATCCATTTGTAAGAACAGGCTCCCCTCGCCGAGTACAGTTATCTCAAAGTCAT
  CCTATCTACATTTCCCCACATAACAACGAAGCAATGCCTTCTCCTCGAGAGAAGATTTTTTACTACTTCA
  GCAACAGCCCATCAAAGAGACTGAGGGAAATCAACAGTATGATACGGACAGGAGAGACTCCAACTAAAAA
  GAGAGGGATTCTCTTGGACGACGGAAGTGAATCACCTGCAAAAAGAATCTGCCCAGAGAATCACTCTGCT
  CTGTTACGTCGTCTCCAGGATGTGGCGAATGACCGAGGTTCACAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211690 representing NM_011250
  Red=Cloning site Green=Tags(s)
  
  MASGGNQSPPPPPAAAASSEEEEEDGDAADRAQPAGSPSHQIQQRFEELCSRLNMDEAARAEAWSSYRSM
  SESYTLEGNDLHWLACALYVACRKSVPTVSKGTAEGNYVSLTRILRCSEQSLIEFFNKMKKWEDMANLPP
  HFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTTSEIFHFCWVLFIYAKG
  NFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGLSEDCHPKDSKASSDPPCVIEKLCSLH
  DGLVLEAKGIKEHFWKPYIRKLFEKKLLKGKEENLTGFLEPGNFGESFKAVNKAYEEYVLAAGNLDERVF
  LGEDAEEEVGTLSRCLSAASGTESAERTQMRDILQQHLDKSKALRVCTPLTGVRYVQENSPCVTPVSTAA
  HSLSRLHTMLSGLRNAPSEKLERILRSCSRDPTQAIADRLKEMYEIYSQHFQPDENFSNCAKEIANKHFR
  FAEMLYYKVLESVIEQEQKRLGDMDLSGVLEHDAFHRSLLACCLEVVAFSHKPPGNFPFIAEIFDVPHYH
  FYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKTKSPLWDRIRDNENRVPTCEEVMPPQNLERTDE
  IYIAGSPLTPRRVGEVRADAGGLGRSITSPTTLYDRYSSPTVSTTRRRLFENDSPSEGSTSGRIPPQPLV
  NAVPVQNVPGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVA
  GSIQPLSAQALAGSLSSQQVTGTTLQVPGPVAIQQISPGGQQQNPGQPLTSSSIRPRKTSSLALFFRKVY
  YLAGVRLRDLCIKLDISDELRKKIWTCFEFSIIQCTELMMDRHLDQLLMCAIYVMAKVTKEDRSFQNIMR
  CYRTQPQARSQVYRSVLIKGKRRNSGSSESRSHQNSPTELNTDRASRDSSPVMRSNSTLPVPQPSSAPPT
  PTRLTGASSDVEEEERGDLIQFYNNIYRKQIQAFAMKYSQANAQTDTPPLSPYPFVRTGSPRRVQLSQSH
  PIYISPHNNEAMPSPREKIFYYFSNSPSKRLREINSMIRTGETPTKKRGILLDDGSESPAKRICPENHSA
  LLRRLQDVANDRGSQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011250
• ORF Size: 4932 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011250.4, NP_035380.3
• RefSeq Size: 4895 bp
• RefSeq ORF: 3408 bp
• Locus ID: 19651
• UniProt ID: Q64700
• Cytogenetics: 8 44.25 cM
• Gene Summary: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.[UniProtKB/Swiss-Prot Function]