Rbl2 (NM_011250) Mouse Untagged Clone

CAT#: MC201180

Rbl2 (untagged) - Mouse retinoblastoma-like 2 (Rbl2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rbl2
• Synonyms: p130; PRB2; Rb2; RBR-2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC020528 sequence for NM_011250
  CGCGGTTTGAATGGCTGCGGGCCCGGGCCCTGAGTGCATCTGAAGAGCCGCGGCCTCGGGGTGCGCCATG GCATCTGGAGGCAACCAGTCGCCACCGCCTCCTCCAGCTGCTGCAGCCAGCTCGGAGGAAGAGGAGGAGG ATGGCGACGCCGCGGATCGCGCGCAGCCCGCGGGGTCCCCGAGCCATCAGATCCAGCAGCGGTTCGAGGA GTTGTGCAGCCGCCTCAACATGGACGAGGCGGCGCGCGCCGAGGCCTGGAGCAGTTACCGCAGCATGAGC GAGAGCTACACGCTGGAGGGAAATGACCTTCATTGGTTAGCATGTGCCTTATATGTGGCTTGCAGAAAAT CTGTTCCAACTGTGAGCAAAGGGACCGCTGAAGGAAACTATGTATCTTTAACCAGAATCCTTCGCTGTTC GGAGCAGAGCCTAATTGAATTTTTTAACAAGATGAAGAAGTGGGAAGACATGGCAAATCTGCCCCCACAT TTCCGAGAACGTACTGAAAGATTAGAAAGAAACTTCACTGTTTCTGCTGTGATTTTTAAGAAATATGAAC CCATTTTTCAAGACATTTTTAAATATCCCCAAGAAGAACAGCCTCGCCAGCAAAGAGGAAGAAAACAGAG GCGACAGCCCTGTACCACATCAGAAATTTTCCATTTTTGCTGGGTGCTTTTTATATATGCGAAAGGGAAC TTCCCCATGATTAGCGATGATCTGGTCAATTCCTACCATCTTCTGCTGTGCGCATTAGATTTAGTCTATG GAAATGCCCTTCAGTGTTCTAACCGTAAAGAACTTGTGAACCCTAATTTTAAAGGCCTGTCCGAGGACTG TCACCCCAAGGACTCTAAAGCGTCCTCCGACCCGCCGTGTGTCATTGAGAAGCTCTGCTCCTTACACGAC GGTCTAGTGTTGGAGGCCAAGGGGATAAAGGAACACTTCTGGAAACCCTATATTAGGAAACTGTTTGAGA AAAAGCTTCTCAAGGGGAAGGAAGAAAATCTTACTGGCTTCCTGGAGCCCGGAAACTTTGGAGAGAGTTT TAAGGCCGTTAATAAGGCATATGAAGAATACGTGTTAGCCGCTGGGAATCTGGATGAACGCGTATTCCTT GGTGAGGATGCTGAGGAGGAAGTTGGGACTCTGTCTCGGTGTCTAAGTGCTGCCTCAGGTACAGAGAGTG CTGAACGGACGCAGATGACAGACATCTTGCAGCAGCATCTTGACAAGTCTAAAGCACTTAGAGTCTGCAC ACCACTGACTGGCGTGAGGTATGTTCAGGAGAACAGCCCGTGTGTGACTCCAGTCTCCACAGCTGCACAC AGCCTGAGCCGTCTTCACAGCATGCTGACCGGCCTCAGGAATGCACCCAGTGAGAAGCTGGAGCAGATAC TCAGGTCATGTTCCCGAGATCCAACTCAGGCTATCGCTGACAGATTGAAAGAAATGTACGAAATATATTC TCAGCATTTCCAGCCAGATGAGAATTTTAGTAATTGTGCTAAAGAAATTGCCAACAAACATTTTCGTTTT GCAGAAATGCTCTACTATAAAGTATTAGAGTCTGTTATTGAGCAAGAACAAAAAAGATTGGGAGACATGG ATTTATCTGGTGTTCTGGAGCATGACGCATTCCACAGGTCACTCTTGGCCTGCTGCCTTGAGGTGGTCGC TTTTTCCCATAAGCCTCCTGGGAATTTTCCATTTATTGCTGAAATATTTGATGTACCACATTATCATTTT TATAAGGTAATTGAAGTATTTATTAGAGCAGAAGACGGTCTTTGCAGAGAAGTGGTCAAACACCTCAATC AGATTGAAGAACAAATTTTAGACCATTTGGCATGGAAAACCAAGTCCCCACTGTGGGACAGAATTAGAGA TAATGAAAACAGAGTCCCTACTTGTGAAGAGGTCATGCCACCTCAAAACCTAGAGAGAACAGATGAAATT TACATCGCTGGCTCTCCCTTAACCCCGAGAAGGGTGGGTGAAGTTCGTGCTGATGCTGGAGGACTTGGAA GAAGTATAACGTCTCCAACCACATTGTATGACAGGTACAGCTCCCCAACAGTCAGCACTACTAGAAGGCG GCTATTCGAGAATGATAGTCCCTCTGAAGGAAGCACATCTGGGCGCATCCCCCCACAACCCCTAGTCAAC GCTGTCCCCGTGCAGAATGTACCTGGGGAGACTGTTTCTGTCACACCAGTTCCTGGACAGACCTTGGTCA CCATGGCAACAGCCACTGTCACGGCCAACAATGGACAAACAGTGACCATTCCAGTCCAAGGTATTGCCAA CGAAAATGGAGGGATAACCTTCTTCCCAGTCCAAGTCAACGTTGGGGGCCAGGCCCAGGCTGTCGCTGGC TCTATCCAGCCCCTCAGTGCTCAAGCACTGGCTGGAAGTCTGAGTTCCCAACAGGTGACAGGAACCACTT TGCAAGTCCCTGGTCCAGTGGCCATTCAACAGATTTCCCCTGGTGGACAACAGCAGAACCCAGGCCAGCC ACTAACCAGCAGCAGTATCCGGCCACGGAAGACTAGCTCCTTAGCGCTCTTCTTTAGAAAGGTTTACTAC TTAGCCGGTGTCCGCCTTCGAGATCTTTGTATAAAACTAGATATTTCAGATGAACTGAGGAAAAAAATTT GGACCTGCTTTGAATTCTCTATAATCCAGTGCACCGAACTTATGATGGACAGACATCTGGACCAGCTGTT GATGTGTGCCATTTATGTGATGGCAAAGGTCACAAAAGAAGACAGGTCCTTCCAGAACATCATGCGTTGT TACAGGACTCAGCCACAGGCCCGGAGCCAGGTGTACAGAAGTGTCTTGATAAAAGGGAAAAGAAGAAACT CTGGCAGCAGTGAGAGCAGAAGCCATCAGAATTCTCCAACCGAACTAAATACAGACAGAGCCAGTAGAGA TTCCAGCCCAGTGATGAGGTCAAACAGCACCCTACCAGTTCCACAGCCCAGCAGTGCCCCTCCTACACCA ACTCGACTCACGGGTGCCAGCAGTGACGTTGAAGAGGAGGAACGAGGAGACCTCATTCAGTTCTACAACA ACATCTATAGGAAGCAAATCCAAGCGTTTGCCATGAAGTACTCGCAGGCAAACTCGCAGACGGACACTCC TCCCCTCTCTCCCTATCCATTTGTAAGAACAGGCTCCCCTCGCCGAGTACAGTTATCTCAAAGTCATCCT ATCTACATTTCCCCACATAACAATGAAGCAATGCCTTCTCCTCGAGAGAAGATTTTTTACTACTTCAGCA ACAGCCCATCAAAGAGACTGAGGGAAATCAACAGCATGATACGGACAGGAGAGACTCCAACTAAAAAGAG AGGGATTCTCTTGGACGACGGAAGTGAATCACCTGCAAAAAGAATCTGCCCAGAGAATCACTCTGCTCTG TTACGTCGTCTCCAGGATGTGGCGAATGACCGAGGTTCACAGTGAGGTTAGTGTCCAGGAGGAAACTGTC TTCACATGAACTGGTTAGCAGGACTTAGTGCATGCAGGACTATGGAACCTTGCTCCTGAATCCAGCAACT GATTAGAGGAGGGGATAAAAGGGAAGCGCTTCTGACTCAGTGTGGCAGCAAATGCCTGGTATCCCATCAC CCAAGGGGTAGGGGACAAGAGGACCAGGAGTTTAAGGCCAACCTGAACAATACAGCATGTCTGAGGGCAA CTTGGACTACGTGTAACCCTTACTCAAAAACAAAGAACCGGAAGGGACGTTTTGGTAAGAAATCAGACTT ATCTCACTGTCCTTAGACTATTTTTCATCCCAGTTGCCTCTTCCTCTACTTAGTGCTTACCTTCAACACG GCTCAGAATCCAAACCTGGGGTTTTGAACTCTGGCAAACTTTTACAAGTACTGCAGGAAGCAAATCTTTA GAGGCTTTTGTAGGTAGGCCCCAGGAGAGGAACTGTATTTAAATTCATTTCCACTTGCATATGGTTAGGT CCAACCATGTGTTTTAGGATGAAAACCCAGAGACATTTACAAACAGAACAAGAGGGGATGGCCCCGACCT GGAAGTGTCCAGGCCTTGGCCTAAGGCTCCTGATCCCTTGAGCACTCACTCTCCCTTCCCCAGTAGGTAC TGTACAGTGTGAAAGCGTGCAATGTCTGAAGGAACCTGTGTAATTGGTGGCACTTTATGGCTGTAAGATG CATAGCATTGTGACCCAGGGTTTGCTGTATATTTATGATGGCACTTTCTATGGTGTGAACTTTGGTAGGT ACAAGCCTTAGGCTAAACAGCTAATAATTTCTTTTAATGCTTTTCTTAAAAGACTGCTGATATAGCTACA TGTTCTGGCCACATGTAAAAAACTTCCATTTGTGGTAGTGGCATTACATAGGGATCATTTAGCTAAGTAA AGATTTTTAAGTCAAGTTGAATTGAGAGTATTTGAAAAGTTTTGACCCCTTCCTTTTGGAAGTAGTTATC CCAAAGAAACTATCTTTGAGGGTATTCCTGGAAGTTAAAAAAATAGGTTGGAGAACTGAGGTTTTTATTA GTACATAGTACCATTTATACAAATTAGAAAATTATTTAACAGCTATTGATTATCTACGCATATCTTTATT AATCATTATTGTTGGATTAATAATCCTAAGGAGAAAATTCAATTGTAAATTGGATCATGATAAACCAAGT TACTAGGTAACTTCATGCTGCTCTACAGCACCCAGCTGAGGACCTACAGCCTGGCACTCCCCCCCCACCA CAGAGTAGTGCTGTGCAGAGTACTTAGAAAACCTTAGTACCGCTACTGTAATTTTATATGAAAATATGTG TATTTTTCAATAAAGCACTTATAAATTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011250
• Insert Size: 3408 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020528, AAH20528
• RefSeq Size: 4872 bp
• RefSeq ORF: 3408 bp
• Locus ID: 19651
• UniProt ID: Q64700
• Cytogenetics: 8 44.25 cM
• Gene Summary: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).