Rnf31 (NM_194346) Mouse Tagged ORF Clone

CAT#: MG211571

Rnf31 (tGFP-tagged) - Mouse ring finger protein 31 (Rnf31)

Product Images

Circular map for MG211571

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rnf31
• Synonyms: AL033293; BC031509; Flj10111; HOIP; mFLJ00217; Paul
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211571 representing NM_194346
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGAGACGAGGAGCGAGGCTTCCTGGCGGCCCGCGAGGAGCTGGCGAGCGCCCTGAGGTGGGATT
  CTGCGCAGGTTTTTCCCCTGGAGCAGCTCATGCCGCTTCTGGCCACCTCTCTGCCACCAGCCGCCCGCTA
  CCTGCAGCTGGACGCCGGACGCTTGGTCCGCTGCAACGCTCATGGGGAGCCTCGAAACTACCTCAACACC
  CTATCCACGGCCCTGAACATCCTGGAAAAATATGGTCGCAACCTCCTCAGCCCGCAGCGGCCCCGGTATT
  GGCGCTCAGTGAAGTTTAATAACCCCGTCTTTCGCAGCACGGTGGATGCTGTGCAGGGTGGCCGGGATGT
  ACTACGGTTGTATGGCTATACTGAGGAGCGCCCAGATGGATTGAGTTTCCCCGAAGGGCAGGAGGAACCA
  GATGAATACCAGGTTGCTGTTGTCACACTAGAAGTACTGCTGCTTCGCACCGAGCTCAGTTTGCTGTTGC
  AGAATACTCATCCCAGACAGAATGCACTGGACCAGCTGCTAAGAGAGAGCGTTGAAGATGGTATGCTGCA
  GCTTTCAGAGTTTCACCCCCTTCTGAGGGAGATTGTTCCTGGCCCCCGCCCCTCTGCCCAAGGCTCCACT
  CCTGGTCCCTGTTTCCTCTGTGGTTCTGCCCCAGGCACACTGCACTGTCCAGCCTGTAACCAAGTCTCGT
  GCCCAGCTTGTGACATTTTGTTCCATGGGCATCCGTCCCGTGCACATCACCTTCGCCAAGCCCTGCCTGG
  GTCCCACCAGACTGCCAGCCTGAGCTCTAGTTTACCTGCCTCGTCCCAACCACGGCCCCCCTCCTCCTCC
  TTGGCCCTGGGAGATAGCTCTCTTTCTTCCCCTGACCCTGCAAATGCCTGTCTGCCCTGGCATTGTCTTA
  CCTGTGCCACACTAAATGAGCCTTGGGCAGTGTTCTGTGCAGTCTGTAGTCAGCCCAAAGGCTGCAAGGT
  GCCGGGAATAGAGGGTTCCCATGGAACCGGGGGCCTAGAACCTGAGCCTGCACGGGATCAATGGGCCTGC
  CAGAGCTGTACCTTTGAGAATGAGGCAGCAGCTGTGCTATGCGCCATATGTGAGCGACCTCGGCTGGCCC
  AGCCTCCCAGCTTGGTGGTGGATTCCCATGATGCTGGTGTTTGCCAACAGTCCCTTAAGCAGGAGGATCC
  TTTGCTCACCGCTGCCCAGCCTCAGGTGTGGTACTGTGACCATTGTACCTTCTGCAATTCAGGCCCTGTC
  TGGGTGTGTGCCATGTGCAACCGAACCCGAGACCCCATCCCTACACAGCCTGCCCTCCAGTCCTATCCCA
  GCTCTTTGGAAAAGGGACGCCCAAAGCCAGGGTCCTCACAACACCTTGGTTCCTCCCTGCCTGCTTCCTG
  TGGAGACCCAGAGAAACAACGCCAAGATAAGATGCGGAAGGAAGGTCTCCAGCTCGTGAGCATGATCCAG
  GAAGGAGAAACTGCGGGTGCCAGTCCAGAAGAGGTCTTCTCAGCTCTCCAATACTCAGGCACAGAGGTGC
  CCCTCCAGTGGTTGCGTTCAGAGCTGTCCTACGTCCTGGAGATGGTGGCTGAGCTTGCTGGACAACAGGA
  TCCAGAGCTGGGGGCCTTTTCCTGTCAGGAAGCCCGGAAAGCCTGGCTTGATCGCCATGGCAACCTGGAT
  GAAGCTGTAGAGGAGTGTGTGAGGGCCAGGAGGAGGAAGGTGCACGAGCTGCAGTCCCTGGGCTTTGGGC
  CTAAGGAAGGGTCACTACAGGCATTGTTCCAGCATGGGGGTGACGTGGCTCGGGCCCTGACTGAGTTACA
  GCGCCAGCGCCTGGAGCCCTTCCATCAGCGCCTATGGGACAGAGACCCTGAACCCACTCCCTGCTGGGAT
  GGGCTGGACAGACAGAGCTTGGTCAGACGCCTTCTGGCCGTCTACACACTCCCCAGCTGGGGCCGAGCAG
  AGCTGGCGCTGGCGCTGCTGCAGGAGACACCCAGGAACTATGAGTTGTTGGACGTGGTGGAGGCTGTGAG
  GCACAGCCAGGACCGGGCCTTTCTGCGTCGACTGCTTGCCCAGGAATGTGCTGTGTGCGGGTGGGCCCTT
  CCCCGCAACCGGATGCAGGCCCTGATCTCCTGTGAGTGCACCATATGTCCCGAATGCTTCCGCCAACACT
  TCACCATTGCCCTGAAGGAGAAGCACATCACAGACATGGTGTGCCCTGCCTGTGGCCGCCCTGACCTCAC
  TGATGACGCTCAGTTACTCAGCTACTTCTCCACCCTTGACATCCAGCTCAGAGAGAGCCTAGACCCCGAT
  GCATATGCCCTGTTTCACAAGAAGCTGACCGAGGCTGTGCTTATGCGAGACCCCAAGTTCTTGTGGTGCG
  CCCAGTGTTCCTTTGGCTTCATCTATGAACGCGAACAGCTGGAGGCGACGTGTCCCCAGTGTCACCAGAC
  CTTCTGTGTGCGCTGCAAGCGCCAGTGGGAGGAGCAGCACAGAGGACGGAGTTGTGAGGATTTCCAGAAC
  TGGAAACGCACCAATGACCCAGAGTACCAGGCTCAAGGCTTGGCCATGTACCTTCAGGAAAACGGCATTG
  ACTGTCCGAAATGCAAGTTCTCGTACGCACTGGCCCGGGGAGGCTGCATGCACTTCCACTGCACGCAGTG
  TCGACACCAGTTCTGCAGTGGCTGCTACAACGCCTTTTACGCCAAGAATAAATGTCCAGACCCTAACTGC
  AAGGTGAAAAAGTCCCTGCATGGCCACCACCCTCGAGACTGCCTCTTCTACCTACGGGACTGGACTGCTG
  CCCGCCTCCAGAAACTGTTGCAGGACAATAATGTCATGTTTAATACAGAGCCTCCAGCTGGGACACGGGC
  AGTCCCTGGAGGGGGCTGCAGAGTGATGGAGCAGAAGGAGGTCCATAGTGGGTTCAGGGATGAAGCTTGC
  GGCAAGGAAACTCCACCTGGCTATGCCGGCCTATGTCAGGCACACTACAAAGAGTATCTCGTGAGCCTCA
  TCAATGCCCATTCACTGGACCCAGCTACCTTGTATGAAGTGGAGGAGCTGGAGACAGCCACTATTCGCTA
  CCTACATTTAGCTCCTCAGCCCGCGGATGGAGAGGATCTGCCTGCTTACCAGGCCCGGCTATTACAGAAG
  CTGAGAGAAGAGGTACCCTTGGGACAGAGTATTGCCCGCAGAAGAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211571 representing NM_194346
  Red=Cloning site Green=Tags(s)
  
  MPGDEERGFLAAREELASALRWDSAQVFPLEQLMPLLATSLPPAARYLQLDAGRLVRCNAHGEPRNYLNT
  LSTALNILEKYGRNLLSPQRPRYWRSVKFNNPVFRSTVDAVQGGRDVLRLYGYTEERPDGLSFPEGQEEP
  DEYQVAVVTLEVLLLRTELSLLLQNTHPRQNALDQLLRESVEDGMLQLSEFHPLLREIVPGPRPSAQGST
  PGPCFLCGSAPGTLHCPACNQVSCPACDILFHGHPSRAHHLRQALPGSHQTASLSSSLPASSQPRPPSSS
  LALGDSSLSSPDPANACLPWHCLTCATLNEPWAVFCAVCSQPKGCKVPGIEGSHGTGGLEPEPARDQWAC
  QSCTFENEAAAVLCAICERPRLAQPPSLVVDSHDAGVCQQSLKQEDPLLTAAQPQVWYCDHCTFCNSGPV
  WVCAMCNRTRDPIPTQPALQSYPSSLEKGRPKPGSSQHLGSSLPASCGDPEKQRQDKMRKEGLQLVSMIQ
  EGETAGASPEEVFSALQYSGTEVPLQWLRSELSYVLEMVAELAGQQDPELGAFSCQEARKAWLDRHGNLD
  EAVEECVRARRRKVHELQSLGFGPKEGSLQALFQHGGDVARALTELQRQRLEPFHQRLWDRDPEPTPCWD
  GLDRQSLVRRLLAVYTLPSWGRAELALALLQETPRNYELLDVVEAVRHSQDRAFLRRLLAQECAVCGWAL
  PRNRMQALISCECTICPECFRQHFTIALKEKHITDMVCPACGRPDLTDDAQLLSYFSTLDIQLRESLDPD
  AYALFHKKLTEAVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQN
  WKRTNDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPDPNC
  KVKKSLHGHHPRDCLFYLRDWTAARLQKLLQDNNVMFNTEPPAGTRAVPGGGCRVMEQKEVHSGFRDEAC
  GKETPPGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATIRYLHLAPQPADGEDLPAYQARLLQK
  LREEVPLGQSIARRRK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194346
• ORF Size: 3198 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194346.3
• RefSeq Size: 3443 bp
• RefSeq ORF: 3201 bp
• Locus ID: 268749
• UniProt ID: Q924T7
• Cytogenetics: 14 C3
• Gene Summary: E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation (PubMed:28701375). LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways (By similarity). Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation (PubMed:28701375). Recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex (By similarity). Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis (By similarity). Binds polyubiquitin of different linkage types (By similarity).[UniProtKB/Swiss-Prot Function]