Rnf31 (NM_194346) Mouse Untagged Clone

CAT#: MC205166

Rnf31 (untagged) - Mouse ring finger protein 31 (Rnf31), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf31
• Synonyms: AL033293; BC031509; Flj10111; HOIP; mFLJ00217; Paul
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057595
  GCGGTGGCTTAAGTGACCCGGGCTGCTGTGGCGCCGCGCGCACCTGCGCCAGCCCCTGGAGGGCAGAGAA GCTGAGCCTGTCTCCAGTCTCAGGATGCCGGGAGACGAGGAGCGAGGCTTCCTGGCGGCCCGCGAGGAGC TGGCGAGCGCCCTGAGGTGGGATTCTGCGCAGGTTTTTCCCCTGGAGCAGCTCATGCCGCTTCTGGCCAC CTCTCTGCCACCAGCCGCCCGCTACCTGCAGCTGGACGCCGGACGCTTGGTCCGCTGCAACGCTCATGGG GAGCCTCGAAACTACCTCAACACCCTATCCACGGCCCTGAACATCCTGGAAAAATATGGTCGCAACCTCC TCAGCCCGCAGCGGCCCCGGTATTGGCGCTCAGTGAAGTTTAATAACCCCGTCTTTCGCAGCACGGTGGA TGCTGTGCAGGGTGGCCGGGATGTACTACGGTTGTATGGCTATACTGAGGAGCGCCCAGATGGATTGAGT TTCCCCGAAGGGCAGGAGGAACCAGATGAATACCAGGTTGCTGTTGTCACACTAGAAGTACTGCTGCTTC GCACCGAGCTCAGTTTGCTGTTGCAGAATACTCATCCCAGACAGAATGCACTGGACCAGCTGCTAAGAGA GAGCGTTGAAGATGGTATGCTGCAGCTTTCAGAGTTTCACCCCCTTCTGAGGGAGATTGTTCCTGGCCCC CGCCCCTCTGCCCAAGGCTCCACTCCTGGTCCCTGTTTCCTCTGTGGTTCTGCCCCAGGCACACTGCACT GTCCAGCCTGTAACCAAGTCTCGTGCCCAGCTTGTGACATTTTGTTCCATGGGCATCCGTCCCGTGCACA TCACCTTCGCCAAGCCCTGCCTGGGTCCCACCAGACTGCCAGCCTGAGCTCTAGTTTACCTGCCTCGTCC CAACCACGGCCCCCCTCCTCCTCCTTGGCCCTGGGAGATAGCTCTCTTTCTTCCCCTGACCCTGCAAATG CCTGTCTGCCCTGGCATTGTCTTACCTGTGCCACACTAAATGAGCCTTGGGCAGTGTTCTGTGCAGTCTG TAGTCAGCCCAAAGGCTGCAAGGTGCCGGGAATAGAGGGTTCCCATGGAACCGGGGGCCTAGAACCTGAG CCTGCACGGGATCAATGGGCCTGCCAGAGCTGTACCTTTGAGAATGAGGCAGCAGCTGTGCTATGCGCCA TATGTGAGCGACCTCGGCTGGCCCAGCCTCCCAGCTTGGTGGTGGATTCCCATGATGCTGGTGTTTGCCA ACAGTCCCTTAAGCAGGAGGATCCTTTGCTCACCGCTGCCCAGCCTCAGGTGTGGTACTGTGACCATTGT ACCTTCTGCAATTCAGGCCCTGTCTGGGTGTGTGCCATGTGCAACCGAACCCGAGACCCCATCCCTACAC AGCCTGCCCTCCAGTCCTATCCCAGCTCTTTGGAAAAGGGACGCCCAAAGCCAGGGTCCTCACAACACCT TGGTTCCTCCCTGCCTGCTTCCTGTGGAGACCCAGAGAAACAACGCCAAGATAAGATGCGGAAGGAAGGT CTCCAGCTCGTGAGCATGATCCAGGAAGGAGAAACTGCGGGTGCCAGTCCAGAAGAGGTCTTCTCAGCTC TCCAATACTCAGGCACAGAGGTGCCCCTCCAGTGGTTGCGTTCAGAGCTGTCCTACGTCCTGGAGATGGT GGCTGAGCTTGCTGGACAACAGGATCCAGAGCTGGGGGCCTTTTCCTGTCAGGAAGCCCGGAAAGCCTGG CTTGATCGCCATGGCAACCTGGATGAAGCTGTAGAGGAGTGTGTGAGGGCCAGGAGGAGGAAGGTGCACG AGCTGCAGTCCCTGGGCTTTGGGCCTAAGGAAGGGTCACTACAGGCATTGTTCCAGCATGGGGGTGACGT GGCTCGGGCCCTGACTGAGTTACAGCGCCAGCGCCTGGAGCCCTTCCATCAGCGCCTATGGGACAGAGAC CCTGAACCCACTCCCTGCTGGGATGGGCTGGACAGACAGAGCTTGGTCAGACGCCTTCTGGCCGTCTACA CACTCCCCAGCTGGGGCCGAGCAGAGCTGGCGCTGGCGCTGCTGCAGGAGACACCCAGGAACTATGAGTT GTTGGACGTGGTGGAGGCTGTGAGGCACAGCCAGGACCGGGCCTTTCTGCGTCGACTGCTTGCCCAGGAA TGTGCTGTGTGCGGGTGGGCCCTTCCCCGCAACCGGATGCAGGCCCTGATCTCCTGTGAGTGCACCATAT GTCCCGAATGCTTCCGCCAACACTTCACCATTGCCCTGAAGGAGAAGCACATCACAGACATGGTGTGCCC TGCCTGTGGCCGCCCTGACCTCACTGATGACGCTCAGTTACTCAGCTACTTCTCCACCCTTGACATCCAG CTCAGAGAGAGCCTAGACCCCGATGCATATGCCCTGTTTCACAAGAAGCTGACCGAGGCTGTGCTTATGC GAGACCCCAAGTTCTTGTGGTGCGCCCAGTGTTCCTTTGGCTTCATCTATGAACGCGAACAGCTGGAGGC GACGTGTCCCCAGTGTCACCAGACCTTCTGTGTGCGCTGCAAGCGCCAGTGGGAGGAGCAGCACAGAGGA CGGAGTTGTGAGGATTTCCAGAACTGGAAACGCACCAATGACCCAGAGTACCAGGCTCAAGGCTTGGCCA TGTACCTTCAGGAAAACGGCATTGACTGTCCGAAATGCAAGTTCTCGTACGCACTGGCCCGGGGAGGCTG CATGCACTTCCACTGCACGCAGTGTCGACACCAGTTCTGCAGTGGCTGCTACAACGCCTTTTACGCCAAG AATAAATGTCCAGACCCTAACTGCAAGGTGAAAAAGTCCCTGCATGGCCACCACCCTCGAGACTGCCTCT TCTACCTACGGGACTGGACTGCTGCCCGCCTCCAGAAACTGTTGCAGGACAATAATGTCATGTTTAATAC AGAGCCTCCAGCTGGGACACGGGCAGTCCCTGGAGGGGGCTGCAGAGTGATGGAGCAGAAGGAGGTCCAT AGTGGGTTCAGGGATGAAGCTTGCGGCAAGGAAACTCCACCTGGCTATGCCGGCCTATGTCAGGCACACT ACAAAGAGTATCTCGTGAGCCTCATCAATGCCCATTCACTGGACCCAGCTACCTTGTATGAAGTGGAGGA GCTGGAGACAGCCACTATTCGCTACCTACATTTAGCTCCTCAGCCCGCGGATGGAGAGGATCTGCCTGCT TACCAGGCCCGGCTATTACAGAAGCTGAGAGAAGAGGTACCCTTGGGACAGAGTATTGCCCGCAGAAGAA AGTAGTAGCAGAGAGCCGGGTCCTGATGGGACTTCCTGACCCAGGCCTCAGCAGCAGTTCCAGCACCAAT AAAGAGGCATCTTATGGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_194346
• Insert Size: 3201 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057595, AAH57595
• RefSeq Size: 3443 bp
• RefSeq ORF: 3201 bp
• Locus ID: 268749
• UniProt ID: Q924T7
• Cytogenetics: 14 C3
• Gene Summary: E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation (PubMed:28701375). LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways (By similarity). Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation (PubMed:28701375). Recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex (By similarity). Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis (By similarity). Binds polyubiquitin of different linkage types (By similarity).[UniProtKB/Swiss-Prot Function]