Card14 (NM_130886) Mouse Tagged ORF Clone

CAT#: MG211416

Card14 (tGFP-tagged) - Mouse caspase recruitment domain family, member 14 (Card14)

Product Images

Circular map for MG211416

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Card14
• Synonyms: Bimp2; CARMA2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211416 representing NM_130886
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAACTGTGCCGCATGGATTCCACACTGACTGCTCTGGACGAGGAGATGCTCTGGGATATGCTGG
  AGAGTCACCGATGCAGGATTGTGCAGAGCATCTGCCCTAGCCGGCTCACCCCCTACCTGCGCCAGGCCAA
  GGTGCTGGGCCAGCTGGACGAGGAGGAGATCCTGCATAGCTCCCGTTTCACCAACAGTGCCATGAGAGTT
  GGGCACTTGCTGGATCTGCTAAAGGCCCGAGGGAAGAATGGAGCCATTGCCTTTCTGGAAAGCCTGAAGT
  TCCACAACCCTGATGTCTACACCCTGGTCACTGGGCTGCAGTCTGACATTGACTTCAGCACTTTCAGCGG
  TCTCATGGAGACATCCAAGCTGACCGAGTGTCTGGCTGGGGCCATCAGCAGCCTGCAGGAGGAGCTGGCC
  CAGGAGAAGGCACAGAAGGAGGTTCTGCTCCGGAGATGCCAGCAGCTGAAGGAGCGCCTGGGCTTGGCTG
  AGGCTCACGCAGAAGGTCTGCGCCAGCTGGAGGTCGACCACAGCCGCATGAAACGTGAGGTCAGCACCCA
  CTTCCACGAGGTCCTGAAACTGAAGGACGAGATGCTGAACCTGTCACTGCACTACAGCAACGCACTCAGA
  GAGAAGGAGCTGGCTGCCACACGCTGCCACAGCCTTCAGGAAGAGCTCTACCTGGTGAAGCAGGAACTCC
  AGCGAGCAAGCCTTGTATCGTCATGTGAAAGAGAATCTCGAGAGAGGTCCCTGAAGATGGCCAGCAACCT
  GGAGCCTCAGGGAGAAGAACTAAATCGGCTTAAGGAGGAGAATGAGAAACTCCGCTCCATGACCTTCAGC
  CTGGTGGAGAAGGACATTCTGGAACAGAGTCTGGATGAGGCCAGGGAGAGCAAGCAAGAGCTGGTGGACC
  GCATCCACTCACTGCGGGAGAGAGCAGTGGCCGCGGAGAGGCAGCAGAAGCAGTACTGGGAGGAGAAGGA
  ACAGACCCTACTCCAGTTCCGGAAGACACAGGTGGACTGTGAACTATACAAAGAAAAGATGACCATGCTT
  CAGGGCCAGGTGGCTGAGCTGCAGAAGGAACGTGACCAGGCATACACAGCAAGGGACAGGGCCCAGATGG
  AGATTTCTCAGCGCCTGGTGGAGAAGGATGCCCTTCGCAGGAGAGTGTTCGAGCTGACGGAGCAGGTCTG
  TGAGCTGCGTACTCAGCTGCGCAGGCTGCAGGCGGAGGCCCCAGGAGGACCCAAGCAGGAAGCCGGAGCC
  AGGGAACTCTGCCTCCGGGGGAAGCAGAGGCTTGTGCGGATGCATGCTGTGTGCCCACCAGATGACAGTG
  ACTGCAGCCTCCTCAGCTCCACAGAGTCCCGGTTGTGGTGGGACCTGAATTCCACATCCAGCCGGGAGCA
  GATGGACAGCTTCCGCTCCAGCAGCCCCATGCCTCCCAGCCAGCAGTCCCTGTACAAGCGGGTGGCAGAG
  GACTTCCTGGAAGACCCCGAGTCTCTAAGCTTCCCAGAAGTCCTTGAGATGCGCCTTCAAGGGGCTACGG
  TGGACGACACAGACACAGACCTGGAGTTTGAGATGATTGATGGAGCAGACCTCTCTCAGACAGAAGACAG
  CCTGCAGGGGTCTTCCCGGAGCCTGAATGTCTCAGAAAGCAGTGTCCCCGTGAGGAGGAGGCCAGCCCGC
  AAGATCCTAAGCCAGGTCACGGTGCTGGCGTTCCAAGGGGACGCGTTGCTGGAACAGATTGGTGTCATCG
  GTGGGAACCTCACTGGGATCTTCATTCACCGTGTCACCCCCGGCTCTGCAGCAGATGAGATGGCCCTGCG
  CCCTGGTACCCAGATCATGATGGTGGACTATAAACCTACCAAGCCATCATTAAGGGCCACTCTGGAGAAT
  ACAACGCTGGAGCAGGCTGTGGGCCTTCTCAGGAGGGTGAATGGCTCCTGCTACTTGTCTGTGAAGATCA
  ACACTGAAGGTTATAAGAACCTCATCCAGGACCTAGATGCCAAAGTGGTAACGTCTGGGGATTCCTTCTA
  CATCCGTGTCAACCTGGCCATGCAGAGGGGAGGGGACGGAGAACTGCAAACTCACTGCAATGACATCCTG
  CATGTCACTGACACCATGTTCCAAGGCCGCAGCTGCTGGCATGCCCACCATGTGAACCCCTACACCATGA
  AAGACATGGAGCCTGGCACCATCCCCAACTATTCACAGGCTCAGCAGCAGCTTCTGGCCCTCATCCAGGA
  CATGACTCAACGGTGCACAGTCCCCCGCAAGCCTCCTGGAGGACCACAGAAGTTAGTACGAATTGTCAGT
  GTGGACAAAGCCGCTGTCAGTCCTCTGACTTCATCCTTTGACCAAAGCCAGTGGGATTCTGGCAAGGAGG
  AAGGTGGTCCTAGCGTGTGCTTCTGGTCAGAGAGCTGCTTCACCCTGGCACCGTACACCCTGGTGCACCC
  CCACAGGCCTGCCCGGCCCCGGCCTGTGCTGTTTGTGCCTAGATTAGTTGGAAGGATCCTGGGTAAAAAG
  CTGTGTCTCCTCCAAGGTTTTAAGCAATGTTCAGCAGAGTACTTGAGCCAGGAGGAGTATGCCACCTGGA
  GTCAGAGAGGGGACATCATCCAGGAGGGAGAGTCAATAGGTGACCACCACTGGATCACCCGGCATGCTGT
  GGAGTCCCTCATGAATATGAGCACCCACGCCCTGCTGGATGTGCGGCTGGACAGCGTCCGAGTCCTACAT
  AGGATGGACATGTTTCCCATTATCATCCATGTCTCTGTCAATGAGAAGACAGCAAAGAAACTCAGGAAGG
  GCCTGCATCGGCTTGGCAGCTCGGAGGAGCAGTTCCTGGAGGTGGCCCGGCAGGAGGAGGGGGAGCTGGA
  CAGAGTGCCGTGTCTGTACAGCAGCCTGGCCCCCGACAGCTGGAGTGATCTGGACAGCCTGCTCAGCTGT
  GTGCGCCTGGCCATTGCAGATGAGCAGAAGAAGGTGGTGTGGACAGAGAGCCCCTGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211416 representing NM_130886
  Red=Cloning site Green=Tags(s)
  
  MAELCRMDSTLTALDEEMLWDMLESHRCRIVQSICPSRLTPYLRQAKVLGQLDEEEILHSSRFTNSAMRV
  GHLLDLLKARGKNGAIAFLESLKFHNPDVYTLVTGLQSDIDFSTFSGLMETSKLTECLAGAISSLQEELA
  QEKAQKEVLLRRCQQLKERLGLAEAHAEGLRQLEVDHSRMKREVSTHFHEVLKLKDEMLNLSLHYSNALR
  EKELAATRCHSLQEELYLVKQELQRASLVSSCERESRERSLKMASNLEPQGEELNRLKEENEKLRSMTFS
  LVEKDILEQSLDEARESKQELVDRIHSLRERAVAAERQQKQYWEEKEQTLLQFRKTQVDCELYKEKMTML
  QGQVAELQKERDQAYTARDRAQMEISQRLVEKDALRRRVFELTEQVCELRTQLRRLQAEAPGGPKQEAGA
  RELCLRGKQRLVRMHAVCPPDDSDCSLLSSTESRLWWDLNSTSSREQMDSFRSSSPMPPSQQSLYKRVAE
  DFLEDPESLSFPEVLEMRLQGATVDDTDTDLEFEMIDGADLSQTEDSLQGSSRSLNVSESSVPVRRRPAR
  KILSQVTVLAFQGDALLEQIGVIGGNLTGIFIHRVTPGSAADEMALRPGTQIMMVDYKPTKPSLRATLEN
  TTLEQAVGLLRRVNGSCYLSVKINTEGYKNLIQDLDAKVVTSGDSFYIRVNLAMQRGGDGELQTHCNDIL
  HVTDTMFQGRSCWHAHHVNPYTMKDMEPGTIPNYSQAQQQLLALIQDMTQRCTVPRKPPGGPQKLVRIVS
  VDKAAVSPLTSSFDQSQWDSGKEEGGPSVCFWSESCFTLAPYTLVHPHRPARPRPVLFVPRLVGRILGKK
  LCLLQGFKQCSAEYLSQEEYATWSQRGDIIQEGESIGDHHWITRHAVESLMNMSTHALLDVRLDSVRVLH
  RMDMFPIIIHVSVNEKTAKKLRKGLHRLGSSEEQFLEVARQEEGELDRVPCLYSSLAPDSWSDLDSLLSC
  VRLAIADEQKKVVWTESPC
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130886
• ORF Size: 2997 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130886.2
• RefSeq Size: 3958 bp
• RefSeq ORF: 3000 bp
• Locus ID: 170720
• UniProt ID: Q99KF0
• Cytogenetics: 11 E2
• Gene Summary: Acts as a scaffolding protein that can activate the inflammatory transcription factor NF-kappa-B and p38/JNK MAP kinase signaling pathways. Forms a signaling complex with BCL10 and MALT1, and activates MALT1 proteolytic activity and inflammatory gene expression. MALT1 is indispensable for CARD14-induced activation of NF-kappa-B and p38/JNK MAP kinases. May play a role in signaling mediated by TRAF2, TRAF3 and TRAF6 and protects cells against apoptosis.[UniProtKB/Swiss-Prot Function]