Spag1 (NM_012031) Mouse Tagged ORF Clone

CAT#: MG211105

Spag1 (tGFP-tagged) - Mouse sperm associated antigen 1 (Spag1)

Product Images

Circular map for MG211105

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Spag1
• Synonyms: tpis
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211105 representing NM_012031
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGCTAAGGCTAAGGACTGTCCATCATTGTGGGGCTTTGGAACAACAAAAACATTTAAAATTCCCA
  TCGAACATTTGGATTTCAAGTATATTGAAAACTGTTCAGATGTGAAACACCTGGAAAAAATTCTTTATGT
  GCTGAGGTCTGGTGAAGAAGGCTACTATCCTGAACTCACGGAATTTTGCGAGAAGTGCCTCACCAACTTG
  GCTCCTAAAAGTCGAGCTCTGAGGAAGGACAAGCCTGCAGAGACAGCATCCAGCTTTTCAGCGGAAGAGT
  GGGAAAAAATCGACAGTGATCTAAAGAGTTGGGTGTCAGAAATCAAAAGAGAGGAAAATACATGTCACTT
  CCATGACCCTGAAAACCATCCCGGAGTGGAAGATCCTCTGCCTCCTGTCCGCGGCTCCACCTGCTGCCCT
  CACAGTGGCAAGGAAACATATTCTAAAAGTAAGACAGCTAAGAAGAGGATTCCAAGGGATTATGCAGAAT
  GGGATAAATTTGATGTGGAAAAAGAATGTTCAAAGATTGATGAGGATTACAAAGAAAAGACTGTCATAAA
  CAACAAGGCTCATCTGTCTAAAATTGAGACAAAAATAGAAACAGCAGGTCTGACTGAGAAGGAAAAGAGT
  TTTCTTGCCAATCGTGAAAAGGGAAAAGGGAACGAGGCTTTCTACTCTGGGGACTATGAAGAGGCCGTGA
  TGTATTACACCAGGAGCTTATCAGCCCTTCCCACCGCCATTGCCTATAACAATCGAGCTCAGGCCGAAAT
  CAAGTTGCAGAGATGGAGCAGTGCTCTTGAAGACTGTGAGAAGGCCTTGGAGCTAGATCCCGGAAACGTA
  AAAGCTCTCCTGAGACGAGCCACTACATATAAACATCAAAACAAGCTCCAGGAAGCTGTGGACGACCTGA
  GAAAGGTGCTGCAGGTGGAGCCGGACAACGACTTGGCCAAGAAGACCTTATCAGAGGTTGAAAGAGATCT
  GAAGAATTCAGAGCCTGTCTCTGAGCTCCAAACCAAAGGGAAGAGGATGGTTATTGAAGAAGTTGAAAAC
  TCGGGAGATGAAGGTGGAAAGGGAAGCGCAGATGAGCGTGAAGATGGTGGCTCGGATGAGGCGGCCATGG
  GCAACATTCAGAAGAAGCTGATGGTCCGCCGGAGCGAGGGTGGCAGGCGGTCGCGGCGGGGCCGGACACC
  GGGGCCCCGAGCTGAGCAGCAGGGAGGCCTGCGAGAGACAGCGACGGCCAGCACCGGCGACAGTCACTAC
  CCGGAGGAGCCGCGAGCCGCTGACAACCCCAGCGGCCTCAAGAGACGGGGCAACGAGCTGTTCCGCGGCG
  GGCAATTCGCAGAGGCAGCCGCGCAATACTCGGTGGCGATAGCGCAGCTGGAACCCACAGGAAGTGCAAA
  TGCGGATGAGCTAAGCATCTTGTATTCAAATAGAGCGGCATGCTACCTCAAAGAAGGGAACTGCAGGGAC
  TGCATCCAGGATTGTAACAGGGCTCTGGAACTTCATCCGTTCTCAGTGAAGCCTCTTCTGCGGCGAGCAA
  TGGCCTATGAGACCTTAGAGCAATACAGGAACGCATATGTGGACTATAAAACAGTGCTGCAGATAGATTG
  TGGGATCCAGCTGGCGAGCGACAGTGCTAACAGAATAGCACGAATCTTAACAGAGCTAGATGGATCAAAA
  TGGCGGGAGAGACTGCCACCCATCCCAGCTGTACCCACATCTGAGCCACTGCGAGTTTGGCTCCCTGCTG
  CAGAGACCCCAGACCAAGATCCCTGTCCCAACAACTGCATGCCGAGCATCACAGATGAAAAAATGTTTCA
  AGCCCTCAAGGAAGAAGGAAATCAACTTGTAAAGGACAAAAATTATAAAGATGCCATTAGTAAATACAAT
  GAATGCTTAAAGATTAACAGCAAGGCATGTGCCATCTATACAAACAGGGCTCTCTGTTACCTGAAGCTGG
  GCCAGTTTGAAGAGGCAAAGCTGGACTGTGAGCAGGCGCTTCAGATAGACGGCGAGAATGTGAAAGCCAG
  CCACAGACTAGCGCTCGCCCAGAAAGGACTCGAGAACTGCCGGGAAAGCGGGGTGGACCCCAGTCAAGTC
  CTCCTAAGCCCAGATTCCAGTGAAGCCGCCCGGCACCTGGACACTAAGAATGACACAGCACCTCCCAGCA
  AAGGAAGGGAGAGGAGGAGAATCCAAGTCCAAGAGGTGGATGGCAGCAGTGATGAGGAGCCTGAGAGACC
  TGCAGAGGCCTCTGCTACCTCCGCACCTGCCAGAGATGGTGTGGAAGATGGAGGGTCTGCAGAACCAGCA
  GAAAAACTCGACGTCTCCAAGCCTACTAATGCCTATGAGTTTGGACAGGTCCTAAGCACTATCAGTGCCA
  GGAAAGATGAAGAGGCCTGTGCACATCTTTTAGCCATCACTGCACCCAAAGACCTGCCACTGTTGCTAAG
  TAACAAACTTGAGGGGGACACGTTCCTCCTCCTCATTCAGTCACTTAAAAGTCACCTTGTGGCTAAAGAC
  CCCTCCCTGGTGTATGAGCATCTCCTGTATCTGAGCAAAGCAGAGAGGTTTAAGACGATGCTGACACTAA
  TTAACAAAGGCCAAAAGGAGCAGATGGCGCAGCTGTTTGATGGTCTGTCAGACACACAGTCCGACGGTTT
  GACGGCAGAGGATGTACAGGCCCTAAGAAGGCAGTATGAGCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211105 representing NM_012031
  Red=Cloning site Green=Tags(s)
  
  MTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNL
  APKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCP
  HSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKS
  FLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNV
  KALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVEN
  SGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHY
  PEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRD
  CIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSK
  WRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYN
  ECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQV
  LLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPA
  EKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKD
  PSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012031
• ORF Size: 2703 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012031.4
• RefSeq Size: 3270 bp
• RefSeq ORF: 2706 bp
• Locus ID: 26942
• UniProt ID: Q80ZX8
• Cytogenetics: 15 B3.1
• Gene Summary: May play a role in the cytoplasmic assembly of the ciliary dynein arms (By similarity). May play a role in fertilization. Binds GTP and has GTPase activity (By similarity).[UniProtKB/Swiss-Prot Function]