Spag1 (NM_012031) Mouse Untagged Clone

CAT#: MC205920

Spag1 (untagged) - Mouse sperm associated antigen 1 (Spag1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spag1
• Synonyms: tpis
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046313
  CTTCAGCCCCCAGCCCAAGCCGGAGGAAGGACTCCCTGCGCCAAAGCTTGCAGGTTGGCCCAAGAGCTAG TTCCCAGGCTCGTCCTAGACACTTTACCTGGGTCTGGCGGTGCCTGGAAACCGTTGCCATGGCTGGGGCG TGCCACTTCCGTTTCTGGCTGCAGAGGGCGCCGGCTGCCTGAGTAGGAGAAGCTGAGTTTTCACGTTACT GGGGTTACCAGGAAACGCTATGCCATGACTGCTAAGGCTAAGGACTGTCCATCATTGTGGGGCTTTGGAA CAACAAAAACATTTAAAATTCCCATCGAACATTTGGATTTCAAGTATATTGAAAACTGTTCAGATGTGAA ACACCTGGAAAAAATTCTTTATGTGCTGAGGTCTGGTGAAGAAGGCTACTATCCTGAACTCACGGAATTT TGCGAGAAGTGCCTCACCAACTTGGCTCCTAAAAGTCGAGCTCTGAGGAAGGACAAGCCTGCAGAGACAG CATCCAGCTTTTCAGCGGAAGAGTGGGAAAAAATCGACAGTGATCTAAAGAGTTGGGTGTCAGAAATCAA AAGAGAGGAAAATACATGTCACTTCCATGACCCTGAAAACCATCCCGGAGTGGAAGATCCTCTGCCTCCT GTCCGCGGCTCCACCTGCTGCCCTCACAGTGGCAAGGAAACATATTCTAAAAGTAAGACAGCTAAGAAGA GGATTCCAAGGGATTATGCAGAATGGGATAAATTTGATGTGGAAAAAGAATGTTCAAAGATTGATGAGGA TTACAAAGAAAAGACTGTCATAAACAACAAGGCTCATCTGTCTAAAATTGAGACAAAAATAGAAACAGCA GGTCTGACTGAGAAGGAAAAGAGTTTTCTTGCCAATCGTGAAAAGGGAAAAGGGAACGAGGCTTTCTACT CTGGGGACTATGAAGAGGCCGTGATGTATTACACCAGGAGCTTATCAGCCCTTCCCACCGCCATTGCCTA TAACAATCGAGCTCAGGCCGAAATCAAGTTGCAGAGATGGAGCAGTGCTCTTGAAGACTGTGAGAAGGCC TTGGAGCTAGATCCCGGAAACGTAAAAGCTCTCCTGAGACGAGCCACTACATATAAACATCAAAACAAGC TCCAGGAAGCTGTGGACGACCTGAGAAAGGTGCTGCAGGTGGAGCCGGACAACGACTTGGCCAAGAAGAC CTTATCAGAGGTTGAAAGAGATCTGAAGAATTCAGAGCCTGTCTCTGAGCTCCAAACCAAAGGGAAGAGG ATGGTTATTGAAGAAGTTGAAAACTCGGGAGATGAAGGTGGAAAGGGAAGCGCAGATGAGCGTGAAGATG GTGGCTCGGATGAGGCGGCCATGGGCAACATTCAGAAGAAGCTGATGGTCCGCCGGAGCGAGGGTGGCAG GCGGTCGCGGCGGGGCCGGACACCGGGGCCCCGAGCTGAGCAGCAGGGAGGCCTGCGAGAGACAGCGACG GCCAGCACCGGCGACAGTCACTACCCGGAGGAGCCGCGAGCCGCTGACAACCCCAGCGGCCTCAAGAGAC GGGGCAACGAGCTGTTCCGCGGCGGGCAATTCGCAGAGGCAGCCGCGCAATACTCGGTGGCGATAGCGCA GCTGGAACCCACAGGAAGTGCAAATGCGGATGAGCTAAGCATCTTGTATTCAAATAGAGCGGCATGCTAC CTCAAAGAAGGGAACTGCAGGGACTGCATCCAGGATTGTAACAGGGCTCTGGAACTTCATCCGTTCTCAG TGAAGCCTCTTCTGCGGCGAGCAATGGCCTATGAGACCTTAGAGCAATACAGGAACGCATATGTGGACTA TAAAACAGTGCTGCAGATAGATTGTGGGATCCAGCTGGCGAGCGACAGTGCTAACAGAATAGCACGAATC TTAACAGAGCTAGATGGATCAAAATGGCGGGAGAGACTGCCACCCATCCCAGCTGTACCCACATCTGAGC CACTGCGAGTTTGGCTCCCTGCTGCAGAGACCCCAGACCAAGATCCCTGTCCCAACAACTGCATGCCGAG CATCACAGATGAAAAAATGTTTCAAGCCCTCAAGGAAGAAGGAAATCAACTTGTAAAGGACAAAAATTAT AAAGATGCCATTAGTAAATACAATGAATGCTTAAAGATTAACAGCAAGGCATGTGCCATCTATACAAACA GGGCTCTCTGTTACCTGAAGCTGGGCCAGTTTGAAGAGGCAAAGCTGGACTGTGAGCAGGCGCTTCAGAT AGACGGCGAGAATGTGAAAGCCAGCCACAGACTAGCGCTCGCCCAGAAAGGACTCGAGAACTGCCGGGAA AGCGGGGTGGACCCCAGTCAAGTCCTCCTAAGCCCAGATTCCAGTGAAGCCGCCCGGCACCTGGACACTA AGAATGACACAGCACCTCCCAGCAAAGGAAGGGAGAGGAGGAGAATCCAAGTCCAAGAGGTGGATGGCAG CAGTGATGAGGAGCCTGAGAGACCTGCAGAGGCCTCTGCTACCTCCGCACCTGCCAGAGATGGTGTGGAA GATGGAGGGTCTGCAGAACCAGCAGAAAAACTCGACGTCTCCAAGCCTACTAATGCCTATGAGTTTGGAC AGGTCCTAAGCACTATCAGTGCCAGGAAAGATGAAGAGGCCTGTGCACATCTTTTAGCCATCACTGCACC CAAAGACCTGCCACTGTTGCTAAGTAACAAACTTGAGGGGGACACGTTCCTCCTCCTCATTCAGTCACTT AAAAGTCACCTTGTGGCTAAAGACCCCTCCCTGGTGTATGAGCATCTCCTGTATCTGAGCAAAGCAGAGA GGTTTAAGACGATGCTGACACTAATTAACAAAGGCCAAAAGGAGCAGATGGCGCAGCTGTTTGATGGTCT GTCAGACACACAGTCCGACGGTTTGACGGCAGAGGATGTACAGGCCCTAAGAAGGCAGTATGAGCTTTAA CTTGGGGTTGCTGAGTTCAAGACAGCAAACATTGTGATGCAGTTGCATGGGTAAAGCCACACCCAGAAGG ATCCCTGTTTGGACATCAGATGTTGTACTTATTTTTATATCTAGAACATGTATATTCTATAATCTGATTT TTATTTATTGTAAATATTCTCTTCTGTACTAGAGCCAACACCTTTATATTTAATAACTATATTCAGAACT TGGCAAATTATACTCTAGCTTATAATACATTTTCTTTTAATGACCTACCCATTAAAAATTTAAAATACAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_012031
• Insert Size: 2706 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC046313, AAH46313
• RefSeq Size: 3270 bp
• RefSeq ORF: 2706 bp
• Locus ID: 26942
• UniProt ID: Q80ZX8
• Cytogenetics: 15 B3.1
• Gene Summary: May play a role in the cytoplasmic assembly of the ciliary dynein arms (By similarity). May play a role in fertilization. Binds GTP and has GTPase activity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1.