Rps6ka4 (NM_019924) Mouse Tagged ORF Clone

CAT#: MG210573

Rps6ka4 (tGFP-tagged) - Mouse ribosomal protein S6 kinase, polypeptide 4 (Rps6ka4)

Product Images

Circular map for MG210573

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rps6ka4
• Synonyms: 90kDa; 1110069D02Rik; AI848992; mMSK2; Msk2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210573 representing NM_019924
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGACGAGGATGAGGACGAGGGCTGCGCCGTGGAGCTGCAGATCACCGAAGCCAACCTCACCGGGC
  ATGAGGAGAAGGTGAGCGTGGAGAACTTCGCGCTGCTCAAGGTGCTGGGCACGGGAGCCTATGGGAAGGT
  GTTCCTGGTGCGGAAGACGGGTGGGCACGACGCGGGCAAGCTCTATGCCATGAAGGTGCTACGCAAGGCG
  GCGTTGGTGCAGCGCGCGAAGACACAGGAGCATACCCGCACCGAACGCTCGGTGCTGGAGCTGGTTCGCC
  AAGCACCCTTCCTGGTCACACTGCACTACGCCTTCCAGACGGATGCCAAGCTGCACCTCATCCTGGACTA
  CGTGAGCGGTGGTGAGATGTTCACTCACCTCTACCAGCGCCAGTACTTCAAGGAGGCTGAGGTTCGAGTG
  TATGGGGGAGAGATTGTGCTGGCCCTGGAACACCTGCACAAGCTGGGTATCATCTACCGGGACCTGAAGC
  TGGAGAACGTCTTACTTGACTCAGAAGGTCACATCGTCCTTACAGACTTTGGGCTGAGCAAGGAGTTCCT
  GACGGAGGAGAAAGAGCGGACCTTCTCCTTCTGTGGCACAATCGAGTACATGGCTCCCGAAATCATCCGA
  AGCAAGGCTGGACATGGCAAGGCTGTGGACTGGTGGAGCCTGGGTATCCTGCTCTTCGAGCTGCTGACAG
  GAGCCTCACCCTTCACACTGGAGGGAGAGAGGAACACTCAGGCTGAGGTGTCCCGACGGATCTTGAAGTG
  CTCCCCTCCCTTCCCTCTCCGGATTGGGCCTGTGGCACAGGACCTGCTACAGCGGCTGCTGTGCAAGGAC
  CCTAAGAAGAGGTTGGGCGCAGGTCCCCAGGGTGCGCAGGAAGTCAAGAGTCACCCCTTCTTCCAGGGTC
  TGGACTGGGTGGCTCTGGCTGCCAGAAAGATCCCAGCCCCATTCCGGCCCCAGATCCGCTCAGAGCTGGA
  TGTGGGGAATTTTGCGGAGGAATTCACCCGGCTGGAGCCCGTCTACCCCCCTGCCGGCAGCCCTCCACCT
  GGGGACCCTCGGATCTTTCAGGGATACTCCTTCGTGGCTCCGTCCATCCTCTTTGACCACAACAATGCAG
  TGATGGCTGATGTACTGCAGGCACCCGGTGCTGGATACAGGCCGGGCAGGGCAGCAGTTGCCAGGAGTGC
  CATGATGCAGGACTCGCCTTTCTTCCAGCAGTACGAACTGGACCTTCGGGAACCAGCACTGGGGCAGGGC
  AGCTTCTCTGTGTGTCGGAGATGTCGGCAGCGCCAGAGCGGCCAGGAGTTTGCTGTCAAGATCCTCAGCC
  GCAGGCTGGAGGAGAACACTCAGAGAGAGGTGGCTGCTCTTCGCCTGTGCCAGTCACACCCCAACGTGGT
  GAATCTGCATGAGGTGCTTCATGACCAGCTACACACTTACCTGGTCCTGGAGTTGCTGCGAGGCGGAGAG
  CTATTGGAACACATCCGCAAGAAGCGGCTCTTCAGCGAGTCCGAGGCCAGCCAGATCCTTCGGAGCCTGG
  TTTCGGCCGTGAGCTTCATGCACGAGGAGGCAGGCGTGGTGCACCGCGACCTGAAACCCGAGAACATCTT
  GTACGCGGACGACACTCCCGGGGCCCCGGTGAAGATCATCGACTTCGGGTTCGCGCGACTGCGGCCCCAG
  AGCCCGGCAGAGCCCATGCAGACTCCTTGCTTCACACTGCAGTACGCTGCACCCGAGCTGCTGGCACAGC
  AGGGCTACGATGAGTCCTGCGATCTATGGAGCCTGGGTGTCATTCTGTACATGATGCTGTCTGGCCAGGT
  TCCCTTCCAAGGGGCCTCGGGCCAGGGTGGACAGAGTCAGGCAGCTGAGATCATGTGCAAGATCCGTGAA
  GGGCGCTTCTCCCTGGACGGGGAAGCCTGGCAAGGTGTGTCGGAGGAAGCCAAGGAGCTGGTCCGAGGGC
  TACTGACAGTCGACCCCGCCAAGCGGCTGAAGCTGGAGGGGCTGCGTAGCAGCTCGTGGCTTCAGGACGG
  CAGCGCGCGCTCCTCGCCCCCGCTCCGCACGCCGGATGTGCTGGAGTCCTCTGGGCCAGCTGTGCGTTCC
  GGGCTCAATGCCACTTTCATGGCGTTCAACCGAGGCAAGCGCGAGGGCTTCTTTCTCAAGAGTGTAGAGA
  ATGCGCCTCTGGCCAAGAGGCGCAAGCAGAAGCTCCGGAGCGCCGCCGCCTCCCGTCGCGGCTCCCCAGT
  GCCTGCCTCCTCCGGTCGCCTACCAGCCTCTGCCGCTAAGGGGACAACTCGCCGAGCCAACGGCCCCTTG
  TCCCCTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210573 representing NM_019924
  Red=Cloning site Green=Tags(s)
  
  MGDEDEDEGCAVELQITEANLTGHEEKVSVENFALLKVLGTGAYGKVFLVRKTGGHDAGKLYAMKVLRKA
  ALVQRAKTQEHTRTERSVLELVRQAPFLVTLHYAFQTDAKLHLILDYVSGGEMFTHLYQRQYFKEAEVRV
  YGGEIVLALEHLHKLGIIYRDLKLENVLLDSEGHIVLTDFGLSKEFLTEEKERTFSFCGTIEYMAPEIIR
  SKAGHGKAVDWWSLGILLFELLTGASPFTLEGERNTQAEVSRRILKCSPPFPLRIGPVAQDLLQRLLCKD
  PKKRLGAGPQGAQEVKSHPFFQGLDWVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYPPAGSPPP
  GDPRIFQGYSFVAPSILFDHNNAVMADVLQAPGAGYRPGRAAVARSAMMQDSPFFQQYELDLREPALGQG
  SFSVCRRCRQRQSGQEFAVKILSRRLEENTQREVAALRLCQSHPNVVNLHEVLHDQLHTYLVLELLRGGE
  LLEHIRKKRLFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQ
  SPAEPMQTPCFTLQYAAPELLAQQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIRE
  GRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRSSSWLQDGSARSSPPLRTPDVLESSGPAVRS
  GLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSAAASRRGSPVPASSGRLPASAAKGTTRRANGPL
  SPS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019924
• ORF Size: 2319 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019924.1, NP_064308.1
• RefSeq Size: 3120 bp
• RefSeq ORF: 2322 bp
• Locus ID: 56613
• UniProt ID: Q9Z2B9
• Cytogenetics: 19 A
• Gene Summary: Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity).[UniProtKB/Swiss-Prot Function]