Rps6ka4 (NM_019924) Mouse Untagged Clone

CAT#: MC200919

Rps6ka4 (untagged) - Mouse ribosomal protein S6 kinase, polypeptide 4 (Rps6ka4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rps6ka4
• Synonyms: 90kDa; 1110069D02Rik; AI848992; mMSK2; Msk2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC012964 sequence for NM_019924
  CCACGCGTCCGCACCCGCCCCGGCCGGAGCCGCCATGTAACCGGCGCCGCCCGTAGCCCCAGCCGCGCGG GCCCCAGCGACCCGCCCGCCATGGGAGACGAGGATGAGGACGAGGGCTGCGCCGTGGAGCTGCAGATCAC CGAAGCCAACCTCACCGGGCATGAGGAGAAGGTGAGCGTGGAGAACTTCGCGCTGCTCAAGGTGCTGGGC ACGGGAGCCTATGGGAAGGTGTTCCTGGTGCGGAAGACGGGTGGGCACGACGCGGGCAAGCTCTATGCCA TGAAGGTGCTACGCAAGGCGGCGTTGGTGCAGCGCGCGAAGACACAGGAGCATACCCGCACCGAACGCTC GGTGCTGGAGCTGGTTCGCCAAGCACCCTTCCTGGTCACACTGCACTACGCCTTCCAGACGGATGCCAAG CTGCACCTCATCCTGGACTACGTGAGCGGTGGTGAGATGTTCACTCACCTCTACCAGCGCCAGTACTTCA AGGAGGCTGAGGTTCGAGTGTATGGGGGAGAGATTGTGCTGGCCCTGGAACACCTGCACAAGCTGGGTAT CATCTACCGGGACCTGAAGCTGGAGAACGTCTTACTTGACTCAGAAGGTCACATCGTCCTTACAGACTTT GGGCTGAGCAAGGAGTTCCTGACGGAGGAGAAAGAGCGGACCTTCTCCTTCTGTGGCACAATCGAGTACA TGGCTCCCGAAATCATCCGAAGCAAGGCTGGACATGGCAAGGCTGTGGACTGGTGGAGCCTGGGTATCCT GCTCTTCGAGCTGCTGACAGGAGCCTCACCCTTCACACTGGAGGGAGAGAGGAACACTCAGGCTGAGGTG TCCCGACGGATCTTGAAGTGCTCCCCTCCCTTCCCTCTCCGGATTGGGCCTGTGGCACAGGACCTGCTAC AGCGGCTGCTGTGCAAGGACCCTAAGAAGAGGTTGGGCGCAGGTCCCCAGGGTGCGCAGGAAGTCAAGAG TCACCCCTTCTTCCAGGGTCTGGACTGGGTGGCTCTGGCTGCCAGAAAGATCCCAGCCCCATTCCGGCCC CAGATCCGCTCAGAGCTGGATGTGGGGAATTTTGCGGAGGAATTCACCCGGCTGGAGCCCGTCTACCCCC CTGCCGGCAGCCCTCCACCTGGGGACCCTCGGATCTTTCAGGGATACTCCTTCGTGGCTCCGTCCATCCT CTTTGACCACAACAATGCAGTGATGGCTGATGTACTGCAGGCACCCGGTGCTGGATACAGGCCGGGCAGG GCAGCAGTTGCCAGGAGTGCCATGATGCAGGACTCGCCTTTCTTCCAGCAGTACGAACTGGACCTTCGGG AACCAGCACTGGGGCAGGGCAGCTTCTCTGTGTGTCGGAGATGTCGGCAGCGCCAGAGCGGCCAGGAGTT TGCTGTCAAGATCCTCAGCCGCAGGCTGGAGGAGAACACTCAGAGAGAGGTGGCTGCTCTTCGCCTGTGC CAGTCACACCCCAACGTGGTGAATCTGCATGAGGTGCTTCATGACCAGCTACACACTTACCTGGTCCTGG AGTTGCTGCGAGGCGGAGAGCTATTGGAACACATCCGCAAGAAGCGGCTCTTCAGCGAGTCCGAGGCCAG CCAGATCCTTCGGAGCCTGGTTTCGGCCGTGAGCTTCATGCACGAGGAGGCAGGCGTGGTGCACCGCGAC CTGAAACCCGAGAACATCTTGTACGCGGACGACACTCCCGGGGCCCCGGTGAAGATCATCGACTTCGGGT TCGCGCGACTGCGGCCCCAGAGCCCGGCAGAGCCCATGCAGACTCCTTGCTTCACACTGCAGTACGCTGC ACCCGAGCTGCTGGCACAGCAGGGCTACGATGAGTCCTGCGATCTATGGAGCCTGGGTGTCATTCTGTAC ATGATGCTGTCTGGCCAGGTTCCCTTCCAAGGGGCCTCGGGCCAGGGTGGACAGAGTCAGGCAGCTGAGA TCATGTGCAAGATCCGTGAAGGGCGCTTCTCCCTGGACGGGGAAGCCTGGCAAGGTGTGTCGGAGGAAGC CAAGGAGCTGGTCCGAGGGCTACTGACAGTCGACCCCGCCAAGCGGCTGAAGCTGGAGGGGCTGCGTAGC AGCTCGTGGCTTCAGGACGGCAGCGCGCGCTCCTCGCCCCCGCTCCGCACGCCGGATGTGCTGGAGTCCT CTGGGCCAGCTGTGCGTTCCGGGCTCAATGCCACTTTCATGGCGTTCAACCGAGGCAAGCGCGAGGGCTT CTTTCTCAAGAGTGTAGAGAATGCGCCTCTGGCCAAGAGGCGCAAGCAGAAGCTCCGGAGCGCCGCCGCC TCCCGTCGCGGCTCCCCAGTGCCTGCCTCCTCCGGTCGCCTACCAGCCTCTGCCGCTAAGGGGACAACTC GCCGAGCCAACGGCCCCTTGTCCCCTTCCTAGCTCCCGCCACTGTGACCCCCTTACCTTTAGGAGCTCCG ATCCGGGAGCCTGGTGCTGCCACCAGACTTTTGCCAGCGGCTCCCACTCTGATCCCTAGGAATTGCCCTC CTCTCCACACCCACTTCCAGACAGAGCAGAAGTATTTTTATAAGCAGAGATTTTTAAAAAGTGTCTTACT AGATAAGAGTTGGGGACACAGAGAAGCAACTGTTGTGAGCAAAGTGGAGCTTGGGGCTCCCTCGCCCGAT CCCAATGCTGCCCCATCAGGAAGAGAGAAGGTCCTTTCCAGGAGCAACTCTGCTAAGAAAGGGCATCCTA TCCTGTTCCTAAGTACCCAGTTGCCATGGAGAAGAAATCAGGAGCCCGTATACTGTGCTTCCCTGGGACT TTGCCCCTAGGAGGGGACACCCATCAATGTCACTTTATGGACCGTGTGCAATGATGTCCACCAAAGACCT GTGTTGGGAATGAGAGAGAGAGAGACCCTGAGGGACCTCTAGAGCATTTCTGAGATACATCACTTTTGTC AACGGCTCCCCTAGTGGGGTTAAGGAAGAAGGCAGGTGCTCCCCAGGGGGAGCCCCTTTCTCACTCACCC ATTCCTCATGGGCACAGCTGCTTCTGGCTGCAGGTGGAGCCTTAGGGTTCTGGGCTAGGCATCCACAGTC ACTGCCTCAGCCCCTCTAGGCTGTGCCTTTGACTTTAAAATAAAACTCCACCCAGTGCTGCAAAAAAAAA AAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_019924
• Insert Size: 2322 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012964, AAH12964
• RefSeq Size: 3156 bp
• RefSeq ORF: 2322 bp
• Locus ID: 56613
• UniProt ID: Q9Z2B9
• Cytogenetics: 19 A
• Gene Summary: Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity).[UniProtKB/Swiss-Prot Function]