Mlh1 (NM_026810) Mouse Tagged ORF Clone

CAT#: MG210511

Mlh1 (tGFP-tagged) - Mouse mutL homolog 1 (E. coli) (Mlh1)

Product Images

Circular map for MG210511

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mlh1
• Synonyms: 1110035C23Rik; AI317206; AI325952; AI561766
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210511 representing NM_026810
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTTGTAGCAGGAGTTATTCGGCGTCTGGACGAGACGGTGGTGAACCGCATAGCGGCGGGGGAAG
  TCATTCAGCGGCCGGCCAATGCTATCAAAGAGATGATAGAAAACTGTTTAGATGCAAAATCTACAAATAT
  TCAAGTGGTTGTTAAGGAAGGTGGCCTGAAGCTAATTCAGATCCAAGACAATGGCACTGGAATCAGGAAG
  GAAGATCTGGATATTGTGTGTGAGAGGTTCACTACGAGTAAACTGCAGACTTTTGAGGATTTAGCCAGTA
  TTTCTACCTATGGCTTTCGTGGTGAGGCATTGGCAAGCATAAGCCATGTGGCCCATGTCACTATTACAAC
  CAAAACAGCTGATGGGAAATGTGCGTACAGAGCAAGTTACTCAGATGGAAAGCTGCAAGCCCCTCCTAAA
  CCCTGTGCAGGCAACCAGGGCACCCTGATCACGGTGGAAGACCTTTTTTACAACATAATCACAAGGAGGA
  AAGCTTTAAAAAATCCAAGTGAAGAGTACGGAAAAATTTTGGAAGTTGTTGGCAGGTATTCAATACACAA
  TTCAGGCATTAGTATCTCAGTTAAAAAACAAGGTGAGACAGTATCTGATGTCAGAACACTGCCCAATGCC
  ACAACCGTGGACAACATTCGCTCCATCTTTGGAAATGCGGTTAGTCGAGAACTGATAGAAGTTGGGTGTG
  AGGATAAAACCCTAGCTTTCAAAATGAATGGCTATATATCGAATGCAAACTATTCAGTGAAGAAGTGCAT
  TTTCCTACTCTTCATCAACCACCGTCTGGTAGAATCAGCTGCCTTGAGAAAAGCCATTGAAACTGTATAT
  GCAGCATATTTGCCCAAAAACACACACCCATTCCTGTACCTCAGTTTGGAAATCAGCCCTCAGAACGTGG
  ACGTCAATGTACACCCCACCAAGCACGAAGTTCACTTTCTGCATGAGGAGAGCATTCTGCAGCGTGTGCA
  GCAGCACATTGAGAGCAAGCTGCTGGGCTCCAATTCCTCCAGGATGTATTTCACCCAGACCTTGCTTCCA
  GGACTTGCTGGGCCCTCTGGGGAGGCAGCTAGACCCACGACAGGGGTGGCTTCCTCATCCACTAGTGGAA
  GTGGCGACAAGGTCTACGCTTACCAGATGGTCCGTACGGACTCCCGGGATCAGAAGCTTGACGCCTTTCT
  GCAGCCTGTAAGCAGCCTTGTGCCCAGCCAGCCCCAGGACCCTGCCCCTGTCCGAGGGGCCAGGACAGAG
  GGCTCTCCTGAAAGGGCCACGCGGGAGGATGAGGAGATGCTTGCTCTCCCAGCCCCCGCTGAAGCAGCTG
  CTGAGAGTGAGAACTTGGAGAGGGAATCACTAATGGAGACTTCAGACACAGCCCAGAAAGCGGCACCCAC
  TTCCAGTCCAGGAAGCTCCAGAAAGAGACATCGGGAGGACTCTGATGTGGAAATGGTGGAAAATGCTTCC
  GGGAAGGAAATGACAGCTGCTTGCTACCCCAGGAGGAGGATCATTAACCTCACCAGCGTCTTGAGTCTCC
  AGGAAGAGATTAGTGAGCGGTGCCATGAGACTCTCCGGGAGATGCTCCGTAACCATTCCTTTGTGGGCTG
  TGTGAATCCTCAGTGGGCCTTGGCACAGCACCAGACCAAGCTATACCTCCTCAACACTACCAAGCTCAGT
  GAAGAGCTGTTCTACCAGATACTCATTTATGATTTTGCCAACTTTGGTGTTCTGAGGTTATCGGAACCAG
  CGCCACTCTTCGACCTGGCCATGCTGGCCTTAGACAGTCCTGAAAGTGGCTGGACAGAGGACGACGGCCC
  GAAGGAAGGGCTTGCAGAGTACATTGTCGAGTTTCTGAAGAAGAAAGCGGAGATGCTTGCAGACTATTTC
  TCTGTGGAGATCGATGAGGAAGGGAACCTGATCGGATTACCTCTTCTGATTGACAGCTATGTGCCACCTT
  TGGAGGGACTGCCTATCTTCATTCTTCGACTGGCCACTGAGGTGAATTGGGATGAAGAAAAGGAGTGTTT
  TGAAAGTCTCAGTAAAGAATGTGCTATGTTTTACTCCATTCGGAAGCAGTATATACTGGAGGAGTCGACC
  CTCTCAGGCCAGCAGAGTGACATGCCTGGCTCCACGTCAAAGCCCTGGAAGTGGACTGTGGAGCACATTA
  TCTATAAAGCCTTCCGCTCACACCTCCTACCTCCGAAGCATTTCACAGAAGATGGCAATGTCCTGCAGCT
  TGCCAACCTGCCAGATCTATACAAAGTCTTTGAGCGGTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210511 representing NM_026810
  Red=Cloning site Green=Tags(s)
  
  MAFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTNIQVVVKEGGLKLIQIQDNGTGIRK
  EDLDIVCERFTTSKLQTFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYRASYSDGKLQAPPK
  PCAGNQGTLITVEDLFYNIITRRKALKNPSEEYGKILEVVGRYSIHNSGISISVKKQGETVSDVRTLPNA
  TTVDNIRSIFGNAVSRELIEVGCEDKTLAFKMNGYISNANYSVKKCIFLLFINHRLVESAALRKAIETVY
  AAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILQRVQQHIESKLLGSNSSRMYFTQTLLP
  GLAGPSGEAARPTTGVASSSTSGSGDKVYAYQMVRTDSRDQKLDAFLQPVSSLVPSQPQDPAPVRGARTE
  GSPERATREDEEMLALPAPAEAAAESENLERESLMETSDTAQKAAPTSSPGSSRKRHREDSDVEMVENAS
  GKEMTAACYPRRRIINLTSVLSLQEEISERCHETLREMLRNHSFVGCVNPQWALAQHQTKLYLLNTTKLS
  EELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEDDGPKEGLAEYIVEFLKKKAEMLADYF
  SVEIDEEGNLIGLPLLIDSYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYILEEST
  LSGQQSDMPGSTSKPWKWTVEHIIYKAFRSHLLPPKHFTEDGNVLQLANLPDLYKVFERC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026810
• ORF Size: 2280 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026810.1, NP_081086.1
• RefSeq Size: 2598 bp
• RefSeq ORF: 2283 bp
• Locus ID: 17350
• UniProt ID: Q9JK91
• Cytogenetics: 9 60.92 cM
• Gene Summary: Heterodimerizes with Pms2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (Msh2-Msh6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of Pms2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (Mlh1-Pms2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with Mlh3 to form MutL gamma which plays a role in meiosis (By similarity).[UniProtKB/Swiss-Prot Function]