Mlh1 (NM_026810) Mouse Untagged Clone

CAT#: MC221356

Mlh1 (untagged) - Mouse mutL homolog 1 (E. coli) (Mlh1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mlh1
• Synonyms: 1110035C23Rik; AI317206; AI325952; AI561766
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221356 representing NM_026810
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTTGTAGCAGGAGTTATTCGGCGTCTGGACGAGACGGTAGTGAACCGCATAGCGGCGGGGGAAG
  TCATTCAGCGGCCGGCCAATGCTATCAAAGAGATGATAGAAAACTGTTTAGATGCAAAATCTACAAATAT
  TCAAGTGGTTGTTAAGGAAGGTGGCCTGAAGCTAATTCAGATCCAAGACAATGGCACTGGAATCAGGAAG
  GAAGATCTGGATATTGTGTGTGAGAGGTTCACTACGAGTAAACTGCAGACTTTTGAGGATTTAGCCAGTA
  TTTCTACCTATGGCTTTCGTGGTGAGGCATTGGCAAGCATAAGCCATGTGGCCCATGTCACTATTACAAC
  CAAAACAGCTGATGGGAAATGTGCGTACAGAGCAAGTTACTCAGATGGAAAGCTGCAAGCCCCTCCTAAA
  CCCTGTGCAGGCAACCAGGGCACCCTGATCACGGTGGAAGACCTTTTTTACAACATAATCACAAGGAGGA
  AAGCTTTAAAAAATCCAAGTGAAGAGTACGGAAAAATTTTGGAAGTTGTTGGCAGGTATTCAATACACAA
  TTCAGGCATTAGTTTCTCAGTTAAAAAACAAGGTGAGACAGTATCTGATGTCAGAACACTGCCCAATGCC
  ACAACCGTGGACAACATTCGCTCCATCTTTGGAAATGCGGTTAGTCGAGAACTGATAGAAGTTGGGTGTG
  AGGATAAAACCCTAGCTTTCAAAATGAATGGCTATATATCGAATGCAAACTATTCAGTGAAGAAGTGCAT
  TTTCCTACTCTTCATCAACCACCGTCTGGTAGAATCAGCTGCCTTGAGAAAAGCCATTGAAACTGTATAT
  GCAGCATACTTGCCCAAAAACACACACCCATTCCTGTACCTCAGTTTGGAAATCAGCCCTCAGAACGTGG
  ACGTCAATGTACACCCCACCAAGCACGAAGTTCACTTTCTGCACGAGGAGAGCATTCTGCAGCGTGTGCA
  GCAGCACATTGAGAGCAAGCTGCTGGGCTCCAATTCCTCCAGGATGTATTTCACCCAGACCTTGCTTCCA
  GGACTTGCTGGGCCTTCTGGGGAGGCAGCTAGACCCACGACAGGGGTGGCTTCCTCATCCACTAGTGGAA
  GTGGCGACAAGGTCTACGCTTACCAGATGGTCCGTACGGACTCCCGGGAGCAGAAGCTTGACGCCTTTCT
  GCAGCCTGTAAGCAGCCTTGGGCCCAGCCAGCCCCAGGACCCTGCCCCTGTCCGAGGGGCCAGGACGGAG
  GGATCTCCTGAAAGGGCCACGCGGGAGGACGAGGAGATGCTTGCTCTCCCAGCCCCCGCTGAAGCAGCTG
  CTGAGAGTGAGAACTTGGAGAGGGAATCACTAATGGAGACTTCAGACGCAGCCCAGAAGGCGGCACCCAC
  TTCCAGTCCAGGAAGCTCCAGAAAGAGACATCGGGAGGACTCTGATGTGGAAATGGTGGAAAATGCTTCC
  GGGAAGGAAATGACAGCTGCTTGCTACCCCAGGAGGAGGATCATTAACCTCACCAGCGTCTTGAGTCTCC
  AGGAAGAGATTAGTGAGCGGTGCCATGAGACTCTCCGGGAGATGCTCCGTAACCATTCCTTTGTGGGCTG
  TGTGAATCCTCAGTGGGCCTTGGCACAGCACCAGACCAAGCTCTACCTCCTCAACACTACCAAGCTCAGT
  GAAGAGCTGTTCTACCAGATACTCATTTATGATTTTGCCAACTTTGGTGTTCTGAGGTTATCGGAACCAG
  CGCCACTCTTCGACCTGGCCATGCTGGCCTTAGACAGTCCTGAAAGTGGCTGGACAGAGGACGACGGCCC
  GAAGGAAGGGCTTGCAGAGTACATTGTTGAGTTTCTGAAGAAGAAAGCGGAGATGCTTGCAGACTATTTC
  TCTGTGGAGATCGATGAGGAAGGGAACCTGATTGGATTACCTCTTCTGATTGACAGCTATGTGCCACCTT
  TGGAGGGACTGCCTATCTTCATTCTTCGACTGGCCACTGAGGTGAATTGGGATGAAGAAAAGGAGTGTTT
  TGAAAGTCTCAGTAAAGAATGTGCTATGTTTTACTCCATTCGGAAGCAGTATATACTGGAGGAGTCGACC
  CTCTCAGGCCAGCAGAGTGACATGCCTGGCTCCACGTCAAAGCCCTGGAAGTGGACTGTGGAGCACATTA
  TCTATAAAGCCTTCCGCTCACACCTCCTACCTCCGAAGCATTTCACAGAAGATGGCAATGTCCTGCAGCT
  TGCCAACCTGCCAGATCTATACAAAGTCTTTGAGCGGTGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026810
• Insert Size: 2283 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026810.2, NP_081086.2
• RefSeq Size: 2598 bp
• RefSeq ORF: 2283 bp
• Locus ID: 17350
• UniProt ID: Q9JK91
• Cytogenetics: 9 60.92 cM
• Gene Summary: Heterodimerizes with Pms2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (Msh2-Msh6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of Pms2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (Mlh1-Pms2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with Mlh3 to form MutL gamma which plays a role in meiosis (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).