Mccc1 (NM_023644) Mouse Tagged ORF Clone

CAT#: MG210254

Mccc1 (tGFP-tagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 1 (alpha) (Mccc1)

Product Images

Circular map for MG210254

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mccc1
• Synonyms: 1810045E08Rik; 2310058B18Rik; Mcca; MCCalpha; R75106
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210254 representing NM_023644
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGTTGTTGGCTGCGGTGGACAGGAACCAGTTGCGACGGGTCCCGATCCTGCTGCTGC
  AGCCGAGGGAATGGGCTTGGAAGTTGAGAACCATGAAGTATGGAACAACCCCAGGAGGAAGCATTACCAA
  GGTCCTCATTGCAAACAGAGGAGAAATTGCCTGCAGGGTGATACGAACAGCCAAAAAGATGGGTGTGCAG
  TCGGTGGCTGTGTACAGTGAGGCCGACCGGAATTCCATGCATGTAGATATGGCAGACGAGGCATATTCCA
  TTGGCCCTGCTCCATCTCAGCAGAGCTACCTCGCAATGGAGAAAATCATTCAAGTGGCCAAGAGCTCTGC
  AGCACAGGCCATCCATCCAGGCTACGGTTTTCTTTCGGAAAACATGGAGTTTGCTGAACTTTGTAAGCAA
  GAGGGAATCATTTTTATAGGTCCTCCTTCGTCTGCAATTAGAGACATGGGTATAAAGAGCACGTCCAAGT
  CCATCATGGCTGCTGCTGGAGTTCCTGTTGTGGAAGGTTACCATGGCAAGGACCAGTCTGATCAGTGCCT
  GAGAGAGCATGCTGGGAAAATCGGGTATCCTGTTATGATCAAAGCCGTCCGTGGTGGAGGAGGAAAAGGC
  ATGAGGATCGTTAGATCAGAGAGAGAATTCCAAGAGCAGTTAGAGTCAGCCCGGAGGGAGGCGAAGAAGT
  CTTTCAATGATGATGCAATGTTGATCGAGAAGTTCGTGGACACTCCAAGGCATGTAGAAGTCCAGGTGTT
  TGGTGATCACCATGGCAACGCAGTGTACTTGTTTGAAAGAGACTGTAGTGTGCAGAGGCGACATCAGAAG
  ATCATTGAGGAGGCCCCAGCGCCTGGTATTAATCCTGAAGTAAGAAGAAAGCTGGGAGAGGCTGCAGTCA
  GAGCTGCGAAGGCTGTCAAGTACGTGGGAGCAGGAACTGTGGAATTCATTATGGACTCAAAGCACAATTT
  TTATTTCATGGAGATGAACACCAGGCTACAAGTGGAACATCCTGTCACTGAAATGATCACAGGGACTGAC
  TTGGTGGAGTGGCAGCTCAGGATTGCAGCAGGAGAGAAGATTCCTTTGAGCCAGGAAGAAATCCCTCTGC
  AGGGCCATGCCTTTGAGGCTAGAATCTATGCAGAAGACCCTGACAATAACTTCATGCCAGGGGCTGGACC
  GTTGGTTCATCTCTCTACCCCTTCGGCAGACATGTCTACGAGGATTGAAACTGGAGTCCGGCAAGGAGAT
  GAAGTCTCGGTGCATTACGATCCCATGATCGCAAAGCTCGTTGTGTGGGCCTCAGACCGCCAGTCAGCCC
  TGTCGAAGCTGAGGTACTGCCTTCATCAGTACAACATTGTCGGCTTGCGCAGCAACGTGGACTTCCTGCT
  CCGGCTCTCCGGCCACCCAGAGTTTGAGGCTGGGAACGTGCACACGGACTTCATCCCTCAGCACCACAAG
  GACCTGCTGCCGAGTCACAGCACTATAGCCAAAGAGTCTGTGTGCCAGGCAGCTCTGGGGCTCATCCTCA
  AAGAGAAAGAAATGACCAGCGCTTTTAAACTCCACACTCAAGATCAATTCTCTCCGTTTTCATTCAGCAG
  TGGGAGAAGACTGAATATCTCTTACACCAGGAACATGACTCTGAGAAGTGGTAAAAGTGATATAGTCATA
  GCTGTGACGTATAACCGCGATGGGTCATATGATATGCAGATTGACAACAAGTCCTTCCGAGTCCTGGGCG
  ATCTTTCTAGTGAAGATGGCTGTACCTACCTGAAGTCTTCTATTAATGGGGTAGCCCGTAAATCCAAGTT
  CATCCTCCTGGACAACACTGTCCACCTGTTTTCTATGGAAGGCAGTATTGAAGTTGGCATTCCAGTGCCC
  AAGTACTTGTCTCCAGTGAGTGCAGAAGGAGCTCAGGGGGGCACCATCGCGCCCATGACTGGAACCATTG
  AAAAGGTGTTTGTGAAAGCTGGAGACAGAGTAAAAGCTGGAGACTCTCTGATGGTTATGATTGCCATGAA
  GATGGAGCATACCATAAAGGCTCCAAAGGATGGCAGGATAAAGAAGGTATTCTTCAGTGAAGGAGCCCAA
  GCCAACAGACATGCACCGTTAGTGGAATTTGAGGAGGAGGAGTCTGACAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210254 representing NM_023644
  Red=Cloning site Green=Tags(s)
  
  MAAAALLAAVDRNQLRRVPILLLQPREWAWKLRTMKYGTTPGGSITKVLIANRGEIACRVIRTAKKMGVQ
  SVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLAMEKIIQVAKSSAAQAIHPGYGFLSENMEFAELCKQ
  EGIIFIGPPSSAIRDMGIKSTSKSIMAAAGVPVVEGYHGKDQSDQCLREHAGKIGYPVMIKAVRGGGGKG
  MRIVRSEREFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQK
  IIEEAPAPGINPEVRRKLGEAAVRAAKAVKYVGAGTVEFIMDSKHNFYFMEMNTRLQVEHPVTEMITGTD
  LVEWQLRIAAGEKIPLSQEEIPLQGHAFEARIYAEDPDNNFMPGAGPLVHLSTPSADMSTRIETGVRQGD
  EVSVHYDPMIAKLVVWASDRQSALSKLRYCLHQYNIVGLRSNVDFLLRLSGHPEFEAGNVHTDFIPQHHK
  DLLPSHSTIAKESVCQAALGLILKEKEMTSAFKLHTQDQFSPFSFSSGRRLNISYTRNMTLRSGKSDIVI
  AVTYNRDGSYDMQIDNKSFRVLGDLSSEDGCTYLKSSINGVARKSKFILLDNTVHLFSMEGSIEVGIPVP
  KYLSPVSAEGAQGGTIAPMTGTIEKVFVKAGDRVKAGDSLMVMIAMKMEHTIKAPKDGRIKKVFFSEGAQ
  ANRHAPLVEFEEEESDK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023644
• ORF Size: 2151 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023644.3
• RefSeq Size: 2429 bp
• RefSeq ORF: 2154 bp
• Locus ID: 72039
• UniProt ID: Q99MR8
• Cytogenetics: 3 B
• Gene Summary: Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.[UniProtKB/Swiss-Prot Function]