Mccc1 (NM_023644) Mouse Untagged Clone

CAT#: MC202933

Mccc1 (untagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 1 (alpha) (Mccc1), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mccc1
• Synonyms: 1810045E08Rik; 2310058B18Rik; Mcca; MCCalpha; R75106
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC021382 sequence for NM_023644
  CCACGCGTCCGAGAAGCTTGCGCAGGGAGGGACGCTTTCAAGAATAAGCCAAGCAGCTTTTGGAATCCAG CAGAACAGCGCGGAGATGGCGGCGGCGGCGTTGTTGGCTGCGGTGGACAGGAACCAGTTGCGACGGGTCC CGATCCTGCTGCTGCAGCCGAGGGAATGGGCTTGGAAGTTGAGAACCATGAAGTATGGAACAACCCCAGG AGGAAGCATTACCAAGGTCCTCATTGCAAACAGAGGAGAAATTGCCTGCAGGGTGATACGAACAGCCAAA AAGATGGGTGTGCAGTCGGTGGCTGTGTACAGTGAGGCCGACCGGAATTCCATGCATGTAGATATGGCAG ACGAGGCATATTCCATTGGCCCTGCTCCATCTCAGCAGAGCTACCTCGCAATGGAGAAAATCATTCAAGT GGCCAAGAGCTCTGCAGCACAGGCCATCCATCCAGGCTACGGTTTTCTTTCGGAAAACATGGAGTTTGCT GAACTTTGTAAGCAAGAGGGAATCATTTTTATAGGTCCTCCTTCGTCTGCAATTAGAGACATGGGTATAA AGAGCACGTCCAAGTCCATCATGGCTGCTGCTGGAGTTCCTGTTGTGGAAGGTTACCATGGCAAGGACCA GTCTGATCAGTGCCTGAGAGAGCATGCTGGGAAAATCGGGTATCCTGTTATGATCAAAGCCGTCCGTGGT GGAGGAGGAAAAGGCATGAGGATCGTTAGATCAGAGAGAGAATTCCAAGAGCAGTTAGAGTCAGCCCGGA GGGAGGCGAAGAAGTCTTTCAATGATGATGCAATGTTGATCGAGAAGTTCGTGGACACTCCAAGGCATGT AGAAGTCCAGGTGTTTGGTGATCACCATGGCAACGCAGTGTACTTGTTTGAAAGAGACTGTAGTGTGCAG AGGCGACATCAGAAGATCATTGAGGAGGCCCCAGCGCCTGGTATTAATCCTGAAGTAAGAAGAAAGCTGG GAGAGGCTGCAGTCAGAGCTGCGAAGGCTGTCAAGTACGTGGGAGCAGGAACTGTGGAATTCATTATGGA CTCAAAGCACAATTTTTATTTCATGGAGATGAACACCAGGCTACAAGTGGAACATCCTGTCACTGAAATG ATCACAGGGACTGACTTGGTGGAGTGGCAGCTCAGGATTGCAGCAGGAGAGAAGATTCCTTTGAGCCAGG AAGAAATCCCTCTGCAGGGCCATGCCTTTGAGGCTAGAATCTATGCAGAAGACCCTGACAATAACTTCAT GCCAGGGGCTGGACCGTTGGTTCATCTCTCTACCCCTTCGGCAGACATGTCTACGAGGATTGAAACTGGA GTCCGGCAAGGAGATGAAGTCTCGGTGCATTACGATCCCATGATCGCAAAGCTCGTTGTGTGGGCCTCAG ACCGCCAGTCAGCCCTGTCGAAGCTGAGGTACTGCCTTCATCAGTACAACATTGTCGGCTTGCGCAGCAA CGTGGACTTCCTGCTCCGGCTCTCCGGCCACCCAGAGTTTGAGGCTGGGAACGTGCACACGGACTTCATC CCTCAGCACCACAAGGACCTGCTGCCGAGTCACAGCACTATAGCCAAAGAGTCTGTGTGCCAGGCAGCTC TGGGGCTCATCCTCAAAGAGAAAGAAATGACCAGCGCTTTTAAACTCCACACTCAAGATCAATTCTCTCC GTTTTCATTCAGCAGTGGGAGAAGACTGAATATCTCTTACACCAGGAACATGACTCTGAGAAGTGGTAAA AGTGATATAGTCATAGCTGTGACGTATAACCGCGATGGGTCATATGATATGCAGATTGACAACAAGTCCT TCCGAGTCCTGGGCGATCTTTCTAGTGAAGATGGCTGTACCTACCTGAAGTCTTCTATTAATGGGGTAGC CCGTAAATCCAAGTTCATCCTCCTGGACAACACTGTCCACCTGTTTTCTATGGAAGGCAGTATTGAAGTT GGCATTCCAGTGCCCAAGTACTTGTCTCCAGTGAGTGCAGAAGGAGCTCAGGGGGGCACCATCGCGCCCA TGACTGGAACCATTGAAAAGGTGTTTGTGAAAGCTGGAGACAGAGTAAAAGCTGGAGACTCTCTGATGGT TATGATTGCCATGAAGATGGAGCATACCATAAAGGCTCCAAAGGATGGCAGGATAAAGAAGGTATTCTTC AGTGAAGGAGCCCAAGCCAACAGACATGCACCGTTAGTGGAATTTGAGGAGGAGGAGTCTGACAAATGAG AATCGCAGTGAACTGTGTCCAGAAAGAAATAGACGACCGCATGGCGCCTTATCTTTCTGCGAACAGCAAG TGCCTCTTCAGTCTCTAGATCTCGGAGAGAGGAGCTTTATTAAATCAATGATGGTGTGATTATATTTAAA CCCTTTTATAAATTATCTTAAAAGATTTCATAGAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_023644
• Insert Size: 2154 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021382, AAH21382
• RefSeq Size: 2429 bp
• RefSeq ORF: 2154 bp
• Locus ID: 72039
• UniProt ID: Q99MR8
• Cytogenetics: 3 B
• Gene Summary: Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.[UniProtKB/Swiss-Prot Function]