Fbxl5 (BC047214) Mouse Tagged ORF Clone

CAT#: MG210023

Fbxl5 (tGFP-tagged) - Mouse F-box and leucine-rich repeat protein 5 (cDNA clone MGC:57022 IMAGE:6466511)

Product Images

Circular map for MG210023

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fbxl5
• Synonyms: Fbl4; Fir4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210023 representing BC047214
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAACAACCAGGAGTTAAAATACGAAATAAAATGGAAGCACCTTGACTGCAGTGTCGTCGAGCTTT
  CTAAAACCAACTTTTCCAACAACAATGACTTCCGTGCTCTTCTCCAGTCTTTATATGCTACTTTCAAGGA
  GTTTAAAATGCATGAACAAATTGAAAATGAATACATTATCGGTTTACTTCAGCAGCGCAGTCAGACCATA
  TATAATGTACACTCTGACAATAAGCTCTCCGAGATGCTGAGTCTCTTTGAAAAGGGGCTGAAGAATGTCA
  AGAATGAGTACGAACAGTTAAATTATGCAAAACAGCTGAAAGAGAGATTGGAGGCTTTTACAAGAGATTT
  TCTTCCTCACATGAAAGAGGAAGAAGAGGTTTTTCAGCCCATGTTAATGGAATATTTTACCTACGAGGAG
  CTTAAAGATATCAAAAAGAAAGTGATTGCACAGCACTGCTCACAGAAGGACACGGCAGAGCTCCTCAGAG
  GGCTCAGTCTGTGGAACCAAGCTGAGGAGCGACAGAAAGTTCTCAAGTACTCTGTGGATGAGAAGGCAGA
  TACAGAAGCGGAAGTGTCAGAACACTCCACAGGTATAACCCATCTTCCTCCTGAGGTAATGCTGTCCATT
  TTCAGTTACCTTAATCCTCAAGAATTGTGTCGGTGTAGTCAAGTCAGTACTAAGTGGTCTCAGCTGGCAA
  AAACAGGATCTTTGTGGAAACATCTTTACCCTGTTCATTGGGCAAGAGGTGACTGGTATAGTGGTCCTGC
  CACTGAACTTGATACTGAGCCTGATGAGGAATGGGTGAGAAATAGGAAAGATGAAAGTCGTGCTTTCCAG
  GAGTGGGATGAAGATGCCGATATAGATGAATCTGAAGAGTCTGCAGAGGAATCGGTTGCTATCAGCATTG
  CACAAATGGAAAAACGTGTACTCCATGGCTTAATTCATAATGTTCTGCCATATGTTGGTACCTCTGTAAA
  AACTTTAGTGTTAGCGTACAGCTCTGCAGTCTCCAGCAAAATGGTTAGGCAGATTTTAGAGCTTTGTCCT
  AACCTGGAACATCTGGATCTTACCCAGACCGACATTTCTGATTCTGCATTTGACAGTTGGTCTTGGCTCG
  GTTGCTGCCAGAGTCTTCGGCATCTTGATCTGTCTGGATGTGAAAAAATCACAGACATGGCTCTAGAGAA
  GATTTCTAGAGCTCTTGGAGTTCTGACATCTCATCAGAGTGGCTTTCTGAAAAGTGCAGGCAAGGCTGCT
  TCAACTCCATGGACAAGTAAAGACATTACCATGCCATCCACCACGCAGTATGCCTGTTTGCACAATTTAA
  CTGACAAAGGCATTGGTGAAGAAATAGACAACGAGCACTCTTGGACTGAACCTGTGTCTTCTGAAAGTTT
  GACTTCTCCTTATGTGTGGATGCTAGATGCTGAAGATTTGGCTGATATTGAAGATGCTGTAGAGTGGAGA
  CACAGAAATGTTGAGAGTCTCTGTGTGATGGAAACAGCCTCCAACTTTGGTTGTTCCTCATCTGGTTGTT
  ACAGTAAGGACATTGTGGGATTAAGGACTAGTGTCTGTTGGCAGCAGCATTGTGCTTCTCCAGCCTTTGC
  ATATTGTGGTCATTCATTCTGTTGTACAGGGACAGCTTTAAGAACTATGACTACACTCCCAGCGACTTCT
  GCAATGTGTAGAAAAGCATTAAGGACTACATTGCCCAGGGGGAAAGACTTAATTTATTTTGGGAGTGAAA
  AGTCTGACCAAGAGACTGGACGAGTACTTTTGTTCCTCAGTCTGTCTGGATGTTACCAGATCACAGACCA
  TGGTCTCAGGGCTCTGACTCTGGGAGGAGGGCTGCCTTACCTGGAGCACCTGAATCTCTCTGGCTGTCTG
  ACTGTAACTGGTGCAGGGCTGCAGGATTTGGTCTCAGCGTGTCCTTCCCTAAATGACGAATACTTTTACT
  ACTGTGACAACATTAACGGTCCTCATGCTGACACCGCCAGTGGATGCCAGAATTTGCAGTGTGGTTTTCG
  AGCCTGCTGCCGCTCTGGCGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210023 representing BC047214
  Red=Cloning site Green=Tags(s)
  
  MRNNQELKYEIKWKHLDCSVVELSKTNFSNNNDFRALLQSLYATFKEFKMHEQIENEYIIGLLQQRSQTI
  YNVHSDNKLSEMLSLFEKGLKNVKNEYEQLNYAKQLKERLEAFTRDFLPHMKEEEEVFQPMLMEYFTYEE
  LKDIKKKVIAQHCSQKDTAELLRGLSLWNQAEERQKVLKYSVDEKADTEAEVSEHSTGITHLPPEVMLSI
  FSYLNPQELCRCSQVSTKWSQLAKTGSLWKHLYPVHWARGDWYSGPATELDTEPDEEWVRNRKDESRAFQ
  EWDEDADIDESEESAEESVAISIAQMEKRVLHGLIHNVLPYVGTSVKTLVLAYSSAVSSKMVRQILELCP
  NLEHLDLTQTDISDSAFDSWSWLGCCQSLRHLDLSGCEKITDMALEKISRALGVLTSHQSGFLKSAGKAA
  STPWTSKDITMPSTTQYACLHNLTDKGIGEEIDNEHSWTEPVSSESLTSPYVWMLDAEDLADIEDAVEWR
  HRNVESLCVMETASNFGCSSSGCYSKDIVGLRTSVCWQQHCASPAFAYCGHSFCCTGTALRTMTTLPATS
  AMCRKALRTTLPRGKDLIYFGSEKSDQETGRVLLFLSLSGCYQITDHGLRALTLGGGLPYLEHLNLSGCL
  TVTGAGLQDLVSACPSLNDEYFYYCDNINGPHADTASGCQNLQCGFRACCRSGE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC047214
• ORF Size: 2052 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC047214.1
• RefSeq Size: 2778 bp
• RefSeq ORF: 2054 bp
• Locus ID: 242960
• Cytogenetics: 5 B3
• Gene Summary: Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued (By similarity).[UniProtKB/Swiss-Prot Function]