Fbxl5 (BC047214) Mouse Untagged Clone

CAT#: MC220930

Fbxl5 (untagged) - Mouse F-box and leucine-rich repeat protein 5 (cDNA clone MGC:57022 IMAGE:6466511), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fbxl5
• Synonyms: Fbl4; Fir4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC047214
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAACAACCAGGAGTTAAAATACGAAATAAAATGGAAGCACCTTGACTGCAGTGTCGTCGAGCTTT
  CTAAAACCAACTTTTCCAACAACAATGACTTCCGTGCTCTTCTCCAGTCTTTATATGCTACTTTCAAGGA
  GTTTAAAATGCATGAACAAATTGAAAATGAATACATTATCGGTTTACTTCAGCAGCGCAGTCAGACCATA
  TATAATGTACACTCTGACAATAAGCTCTCCGAGATGCTGAGTCTCTTTGAAAAGGGGCTGAAGAATGTCA
  AGAATGAGTACGAACAGTTAAATTATGCAAAACAGCTGAAAGAGAGATTGGAGGCTTTTACAAGAGATTT
  TCTTCCTCACATGAAAGAGGAAGAAGAGGTTTTTCAGCCCATGTTAATGGAATATTTTACCTACGAGGAG
  CTTAAAGATATCAAAAAGAAAGTGATTGCACAGCACTGCTCACAGAAGGACACGGCAGAGCTCCTCAGAG
  GGCTCAGTCTGTGGAACCAAGCTGAGGAGCGACAGAAAGTTCTCAAGTACTCTGTGGATGAGAAGGCAGA
  TACAGAAGCGGAAGTGTCAGAACACTCCACAGGTATAACCCATCTTCCTCCTGAGGTAATGCTGTCCATT
  TTCAGTTACCTTAATCCTCAAGAATTGTGTCGGTGTAGTCAAGTCAGTACTAAGTGGTCTCAGCTGGCAA
  AAACAGGATCTTTGTGGAAACATCTTTACCCTGTTCATTGGGCAAGAGGTGACTGGTATAGTGGTCCTGC
  CACTGAACTTGATACTGAGCCTGATGAGGAATGGGTGAGAAATAGGAAAGATGAAAGTCGTGCTTTCCAG
  GAGTGGGATGAAGATGCCGATATAGATGAATCTGAAGAGTCTGCAGAGGAATCGGTTGCTATCAGCATTG
  CACAAATGGAAAAACGTGTACTCCATGGCTTAATTCATAATGTTCTGCCATATGTTGGTACCTCTGTAAA
  AACTTTAGTGTTAGCGTACAGCTCTGCAGTCTCCAGCAAAATGGTTAGGCAGATTTTAGAGCTTTGTCCT
  AACCTGGAACATCTGGATCTTACCCAGACCGACATTTCTGATTCTGCATTTGACAGTTGGTCTTGGCTCG
  GTTGCTGCCAGAGTCTTCGGCATCTTGATCTGTCTGGATGTGAAAAAATCACAGACATGGCTCTAGAGAA
  GATTTCTAGAGCTCTTGGAGTTCTGACATCTCATCAGAGTGGCTTTCTGAAAAGTGCAGGCAAGGCTGCT
  TCAACTCCATGGACAAGTAAAGACATTACCATGCCATCCACCACGCAGTATGCCTGTTTGCACAATTTAA
  CTGACAAAGGCATTGGTGAAGAAATAGACAACGAGCACTCTTGGACTGAACCTGTGTCTTCTGAAAGTTT
  GACTTCTCCTTATGTGTGGATGCTAGATGCTGAAGATTTGGCTGATATTGAAGATGCTGTAGAGTGGAGA
  CACAGAAATGTTGAGAGTCTCTGTGTGATGGAAACAGCCTCCAACTTTGGTTGTTCCTCATCTGGTTGTT
  ACAGTAAGGACATTGTGGGATTAAGGACTAGTGTCTGTTGGCAGCAGCATTGTGCTTCTCCAGCCTTTGC
  ATATTGTGGTCATTCATTCTGTTGTACAGGGACAGCTTTAAGAACTATGACTACACTCCCAGCGACTTCT
  GCAATGTGTAGAAAAGCATTAAGGACTACATTGCCCAGGGGGAAAGACTTAATTTATTTTGGGAGTGAAA
  AGTCTGACCAAGAGACTGGACGAGTACTTTTGTTCCTCAGTCTGTCTGGATGTTACCAGATCACAGACCA
  TGGTCTCAGGGCTCTGACTCTGGGAGGAGGGCTGCCTTACCTGGAGCACCTGAATCTCTCTGGCTGTCTG
  ACTGTAACTGGTGCAGGGCTGCAGGATTTGGTCTCAGCGTGTCCTTCCCTAAATGACGAATACTTTTACT
  ACTGTGACAACATTAACGGTCCTCATGCTGACACCGCCAGTGGATGCCAGAATTTGCAGTGTGGTTTTCG
  AGCCTGCTGCCGCTCTGGCGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC047214
• Insert Size: 2055 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC047214, AAH47214
• RefSeq Size: 2778 bp
• RefSeq ORF: 2054 bp
• Locus ID: 242960
• Cytogenetics: 5 B3
• Gene Summary: Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued (By similarity).[UniProtKB/Swiss-Prot Function]