Wee1 (NM_009516) Mouse Tagged ORF Clone

CAT#: MG209724

Wee1 (tGFP-tagged) - Mouse wee 1 homolog (S. pombe) (Wee1)

Product Images

Circular map for MG209724

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wee1
• Synonyms: Wee1A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209724 representing NM_009516
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCCTGAGCCGACAGCAGCCGCCGCCCACCCGCCGCGTAGGAGCCGCTTACAGTCTGCGGCAGA
  AGCTCATCTTCTCGCCGGGCAGCGACTGTGAGGAAGAGGAGGAGGAGGAGGAGGAAGGCAGCGGCCACAG
  TACGGGGGAGGACTCGGCCTTCCAGGAACCGGATTCGCCCCTGCCTTCTGCACGCAGCCCTGCCGAGGCC
  GAGGCCGAGCGCCGCCGTCGCTCGCCCGGCGCGGAGCCCAGCAGCCCGGGAGAGCTGGAGGACGACTTGC
  TGCTGCAGGGCGGCGGCGGAGGCGCGCAAGCGGCGGGTGGAGGCGCCGAGGGCGACTCTTGGGAGGAGGA
  GGGTTTCGGCTCCTCGTCGCCCGTCAAATCACCTTCGACCGCCTACTTTCTGAGCAGCCCGTTCTCGCCA
  GTGCGCTGCGGCGGCCCAGGGGATGCCTCCCCGCAGGGTTGCGGGGCTCCGCGAGCCATGGACGACCCCT
  GCTCGCCGCAGCCCGACTACCCGAGCACCCCTCCGCACAAGACCTTCCGCAAGCTGCGCCTGTTCGACAC
  GCCGCACACTCCCAAGAGTTTGCTTTCCAAAGCGCGAGTTATTGATTCCGGCTCTGTTAAACTCCGGGGT
  AGTTCTCTATTCATGGACACAGAAAAGTCAGGAAAAAGAGAATTCGATACACGGCAAACTCCCCAAGTGA
  ATATTAACCCCTTTACTCCGGATCCTGTACTGCTCCATTCCTCAGGACGGTGTCGTGGGAGAAAGAGAGC
  GTATTTTAATGATTCCTCTGAAGACATGGAAGCCAGTGATTATGAGTTTGAAGATGAAACAAGACCTGCC
  AAAAGAATTACAATTACTGAAAGCAACATGAAGTCACGGTATACAACTGAATTTCATGAGCTGGAGAAAA
  TTGGTTCTGGAGAATTTGGTTCTGTGTTTAAATGTGTGAAGAGGTTAGATGGATGCATTTATGCCATTAA
  ACGATCAAAAAAACCATTGGCTGGCTCTGTTGATGAGCAGAATGCTTTGAGAGAAGTGTATGCTCACGCT
  GTGCTTGGACAGCACCCCCACGTCGTTCGCTATTTCTCTGCCTGGGCAGAGGATGACCACATGCTTATAC
  AGAACGAATACTGTAATGGTGGGAGTTTAGCTGATGCTATAAGTGAGAACTACAGAGTCATGAGCTACTT
  GACTGAAGTAGAGCTGAAGGATCTCCTTTTGCAAGTTGGCCGGGGCTTGAGATACATACATTCAATGTCT
  TTGGTTCACATGGATATAAAACCTAGTAATATTTTTATATCTCGAACCTCAATCCCAAATGCTGTCTCTG
  AGGAAGGAGATGAAGATGACTGGATATCCAATAAAGTTATGTTTAAAATAGGTGATCTTGGGCATGTAAC
  AAGAATCTCTAGTCCTCAAGTTGAAGAAGGTGATAGTCGTTTTCTAGCAAATGAAGTTTTACAAGAGAAC
  TATAGCCATCTACCGAAAGCAGATATTTTTGCTCTTGCTCTCACAGTCGTATGTGCTGCTGGTGCTGAAC
  CCCTTCCCAGAAATGGAGAGCAGTGGCACGAGATCCGGCAGGGTCGGTTACCTCGGATCCCACAAGTGCT
  TTCCCAGGAGGTGACAGAGTTGCTGAGAGTCATGATTCATCCTGACCCAGAGAGAAGGCCTTCAGCAATG
  GAGCTGGTGAAGCATTCAGTGTTGTTGTCTGCATCTAGAAAGAGCGCAGAGCAGTTACGAATAGAATTGA
  ATGCTGAAAAATTCAAAAATTCTCTTTTGCAGAAAGAACTCAAGAAAGCCCAAATGGCAGCCAAAGTTGC
  AGCTGAGGAACGGGCACTCTTCACAGATCGGATGGCCACTAGATCCACCACCCAGAGTAATAGAACTTCT
  CGACTTATTGGAAAGAAAATGAACCGCTCTGTCAGCCTTACTATATAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209724 representing NM_009516
  Red=Cloning site Green=Tags(s)
  
  MSFLSRQQPPPTRRVGAAYSLRQKLIFSPGSDCEEEEEEEEEGSGHSTGEDSAFQEPDSPLPSARSPAEA
  EAERRRRSPGAEPSSPGELEDDLLLQGGGGGAQAAGGGAEGDSWEEEGFGSSSPVKSPSTAYFLSSPFSP
  VRCGGPGDASPQGCGAPRAMDDPCSPQPDYPSTPPHKTFRKLRLFDTPHTPKSLLSKARVIDSGSVKLRG
  SSLFMDTEKSGKREFDTRQTPQVNINPFTPDPVLLHSSGRCRGRKRAYFNDSSEDMEASDYEFEDETRPA
  KRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHA
  VLGQHPHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRVMSYLTEVELKDLLLQVGRGLRYIHSMS
  LVHMDIKPSNIFISRTSIPNAVSEEGDEDDWISNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQEN
  YSHLPKADIFALALTVVCAAGAEPLPRNGEQWHEIRQGRLPRIPQVLSQEVTELLRVMIHPDPERRPSAM
  ELVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAAKVAAEERALFTDRMATRSTTQSNRTS
  RLIGKKMNRSVSLTIY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009516
• ORF Size: 1938 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009516.3, NP_033542.2
• RefSeq Size: 3419 bp
• RefSeq ORF: 1941 bp
• Locus ID: 22390
• UniProt ID: P47810
• Cytogenetics: 7 E3
• Gene Summary: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation (By similarity).[UniProtKB/Swiss-Prot Function]