Wee1 (NM_009516) Mouse Untagged Clone

CAT#: MC205607

Wee1 (untagged) - Mouse WEE 1 homolog 1 (S. pombe) (Wee1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wee1
• Synonyms: Wee1A
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC006852
  CGGACGCGTGGGCGGACGCGTGGGGGGAAGCCGCGGAACCCGAACTTGCGGACGAACTTGCGGAGCCTTT CGCCAGCACTGGACCGGCCAGTCTCCGCTGGACATCCCGCGCCGCAGCCGTCGCCGTCGCCCCGTCGTGA GCTTGACGGGCCTCGGAGTTGCCGCCGCGCAGGCCCGCTGCAGCCTCAGCCCGGCGCGCCCGATCGCACG TGGCGGACCCGCCCCCTGTGCCAGCCGCGACCTGGACGCCGCCGGCCTCTGCTCGCCGGTCCTGTGTTCT CCCGTGCTCCGCCGTGACTGCAGCCGGGAAATGAGCTTCCTGAGCCGACAGCAGCCGCCGCCCACCCGCC GCGTAGGAGCCGCTTACAGTCTGCGGCAGAAGCTCATCTTCTCGCCGGGCAGCGACTGTGAGGAAGAGGA GGAGGAGGAGGAGGAAGGCAGCGGCCACAGTACGGGGGAGGACTCGGCCTTCCAGGAACCGGATTCGCCC CTGCCTTCTGCACGCAGCCCTGCCGAGGCCGAGGCCGAGCGCCGCCGTCGCTCGCCCGGCGCGGAGCCCA GCAGCCCGGGAGAGCTGGAGGACGACTTGCTGCTGCAGGGCGGCGGCGGAGGCGCGCAAGCGGCGGGTGG AGGCGCCGAGGGCGACTCTTGGGAGGAGGAGGGTTTCGGCTCCTCGTCGCCCGTCAAATCACCTTCGACC GCCTACTTTCTGAGCAGCCCGTTCTCGCCAGTGCGCTGCGGCGGCCCAGGGGATGCCTCCCCGCAGGGTT GCGGGGCTCCGCGAGCCATGGACGACCCCTGCTCGCCGCAGCCCGACTACCCGAGCACCCCTCCGCACAA GACCTTCCGCAAGCTGCGCCTGTTCGACACGCCGCACACTCCCAAGAGTTTGCTTTCCAAAGCGCGAGTT ATTGATTCCGGCTCTGTTAAACTCCGGGGTAGTTCTCTATTCATGGACACAGAAAAGTCAGGAAAAAGAG AATTCGATACACGGCAAACTCCCCAAGTGAATATTAACCCCTTTACTCCGGATCCTGTACTGCTCCATTC CTCAGGACGGTGTCGTGGGAGAAAGAGAGCGTATTTTAATGATTCCTCTGAAGACATGGAAGCCAGTGAT TATGAGTTTGAAGATGAAACAAGACCTGCCAAAAGAATTACAATTACTGAAAGCAACATGAAGTCACGGT ATACAACTGAATTTCATGAGCTGGAGAAAATTGGTTCTGGAGAATTTGGTTCTGTGTTTAAATGTGTGAA GAGGTTAGATGGATGCATTTATGCCATTAAACGATCAAAAAAACCATTGGCTGGCTCTGTTGATGAGCAG AATGCTTTGAGAGAAGTGTATGCTCACGCTGTGCTTGGACAGCACCCCCACGTCGTTCGCTATTTCTCTG CCTGGGCAGAGGATGACCACATGCTTATACAGAACGAATACTGTAATGGTGGGAGTTTAGCTGATGCTAT AAGTGAGAACTACAGAGTCATGAGCTACTTGACTGAAGTAGAGCTGAAGGATCTCCTTTTGCAAGTTGGC CGGGGCTTGAGATACATACATTCAATGTCTTTGGTTCACATGGATATAAAACCTAGTAATATTTTTATAT CTCGAACCTCAATCCCAAATGCTGTCTCTGAGGAAGGAGATGAAGATGACTGGATATCCAATAAAGTTAT GTTTAAAATAGGTGATCTTGGGCATGTAACAAGAATCTCTAGTCCTCAAGTTGAAGAAGGTGATAGTCGT TTTCTAGCAAATGAAGTTTTACAAGAGAACTATAGCCATCTACCGAAAGCAGATATTTTTGCTCTTGCTC TCACAGTCGTATGTGCTGCTGGTGCTGAACCCCTTCCCAGAAATGGAGAGCAGTGGCACGAGATCCGGCA GGGTCGGTTACCTCGGATCCCACAAGTGCTTTCCCAGGAGGTGACAGAGTTGCTGAGAGTCATGATTCAT CCTGACCCAGAGAGAAGGCCTTCAGCAATGGAGCTGGTGAAGCATTCAGTGTTGTTGTCTGCATCTAGAA AGAGCGCAGAGCAGTTACGAATAGAATTGAATGCTGAAAAATTCAAAAATTCTCTTTTGCAGAAAGAACT CAAGAAAGCCCAAATGGCAGCCAAAGTTGCAGCTGAGGAACGGGCACTCTTCACAGATCGGATGGCCACT AGATCCACCACCCAGAGTAATAGAACTTCTCGACTTATTGGAAAGAAAATGAACCGCTCTGTCAGCCTTA CTATATACTGAACTGCTCACATTCCCCAGCCCTTCCCCACTCAAAGGAAAAAACTGTGACAAGAGGAAGC TAGGTTGGAATCACTGCTAGAGCCCACTTCGTGATTACTTGCCCATTGGTATCAGTAGTTTTCCTAAAAC GTAACGCCTTCTGTGACTTTGAGAATTACAGTTGAGAGCTGTATTCTGACTTGGCTCTGTCAGGCTTTTG TCTCATCTCCCGTCTGTTTCATGTAGGATGTGGGTCACTGTTGGATGTTCCAGCCCCCTTTGCGGGGTGC GCCACTGTGGTGGTGTGCTGCTTCTCATTCACTGTTGCAGTGTGGTGAAAGGTGTCTTTCCTGGTTGTGA CCTCTAGAAAGGACTCAAGGGCTTTACTGCGAACATCCTCTCCCTTCAAAAAGGGAAATGCTCAGAGACT CAGTACTCCTCTGCCTCCAATGTACCTGTGTCAGTCTTGCGTTTCTTTTTTCTATGTGAATTACACTTGT ATATCCAACTGGGAGCACTTTAGGCATCGTGTGCGCCATGGGATGGTCATTCTGTGGAGGCCTTGCCTTG TGAATTTGCTGCTATTTTAGTTTCCTCTTGCTGTAAAACTAGCATTAAACAATCATTGTTGTTAATAGGC TTTCTTTCAGAACAATTATGTGAAATATATAGCTGCTCTTCATGAAAAGCAGCTTCGCATCTTTTTCTTT GGACTTTGAAGCTACTGCATTGAAAAAGTGCACCTACCCCTCCCTGTTTGGATGCTGTATTAATGTAGTA TAATTATTACTGGTTTTGTAAATTTTTCTTATAATGTCCCCTGTCTTTAAAACATGTTTCCTTCCTCCTG TCAAGTCTTGTACTTGGTTGTATGGTTAGCCTATTTTTAAATGTTTTGCCCAGTTTCTCTTCAATATTTG TGTATATAAACTGATCTTGGTAATACTGTACATAGCTGTTTAAAAATACCAGAATGACTTCTGACTATTC CAAGTTTTCCACAAAATACATTTTACCTGTGAATAGCCATTTGACTAACAATATTTGGCTAGTAGATGCT GCCCTCCAAAAGGAGGGAAATGTCTCACTTGTTTTCCTATTAACTTTGGTCTGAAACACATTTGCACTTG TCTGTTTGACTTGTGTCTTATTAACATTGTCTGGTGTATTAAAAGTCATTCCGTGCTTACCTTAAAAAAA AAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_009516
• Insert Size: 1941 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006852, AAH06852
• RefSeq Size: 3438 bp
• RefSeq ORF: 1941 bp
• Locus ID: 22390
• UniProt ID: P47810
• Cytogenetics: 7 E3
• Gene Summary: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation (By similarity).[UniProtKB/Swiss-Prot Function]