Arfgap3 (NM_025445) Mouse Tagged ORF Clone

CAT#: MG208404

Arfgap3 (tGFP-tagged) - Mouse ADP-ribosylation factor GTPase activating protein 3 (Arfgap3)

Product Images

Circular map for MG208404

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arfgap3
• Synonyms: 0610009H19Rik; 1810004P07Rik; 1810035F16Rik; 9130416J18Rik; Arfgap1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208404 representing NM_025445
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACCCCAGCAAGCAGGACATCCTGGCCATCTTCAAGCGCCTGCGCTCGGTGCCCACTAACAAGG
  TGTGTTTTGACTGTGGTGCCAAAAATCCCAGCTGGGCCAGCATAAGCTATGGGGTGTTTCTCTGCATTGA
  CTGCTCTGGATCACACCGGTCGCTCGGGGTTCACTTGAGTTTTATTCGGTCCACAGAGCTGGATTCAAAC
  TGGTCGTGGTTCCAGCTTCGATGCATGCAAGTTGGAGGGAACTCAAATGCTTCTTCCTTCTTCCACCAAC
  ATGGGTGTGCCACCAAGGACACCAACGCCAAGTACAACAGCCGTGCGGCTCAGCTCTACAGGGAGAAGAT
  CAAGACTCTGGCCACCCAGGCCACCCGCAGGCACGGCACCGATCTGTGGCTGGACAGCTGTGCGGCTCCA
  CCTGTGTCCCCTCCTCCAAAGGAGGAAGATTTTTTTGCTTCTCACGCCTCTCTGGAGGTCAGTGGTGCTA
  TGCAGGCATCCGCACAGCCAGAGTCAGCCTCTTCAACACCGTGGGGCTTGGAAACCACTCCAGAAAAACA
  TGAAGGTGGGCCAGGACAAGGGCCAAGCGTGGAAGGTCTGAACACCCCTGGAAAGGCTGCACCAGAGGTT
  TCATCGATTATAAAAAAGAAGCCAAATCAAGCAAAAAAAGGACTTGGAGCCAAAAAAGGAAGCTTGGGAG
  CCCAGAAACTGACAAATACATCCTTCACTGAGATCGAAAAGCAAGCTCAGGCTGTGGATAAGAGGAAGGA
  GCAGGAAGACCTTGCCAGGGGTGCCCCAAAGGAGGAGTCTATTGTTTCGTCCTTGCGACTGGCCTACAAG
  GATCTGGAAATCAGCAGGAAGAAAGATGAAAGACTGAACCTGAGTGGCCAGAAGAAGGTGGAAGCCGAGC
  GGCTGGGCATGGGCTTTGGAAGCTGCAGGAGTGGCATCTCCCACTCGGTGACTTCAGACATGCAGACCAT
  TGAGCAGGAGTCGCCCACACTGGCAAAGCCGAGGAGGAAGTACCAGGAGGACCCAGAGGACTCGTACTTC
  AGCTCCAGCTCCAAGTGGTCTGAGCAGAGCAGCAGCAGGTACTTTGACGACCCCATGGAGTTAAGAAGCA
  GCTCCTTCTCTAGCTGGGATGATGGTGCCGATTCCTACTGGAAGAAAGACAGCAGCAGAGACCCCGAGCC
  TGCCATGAGAAGCACCGGCTCTTCAGACAGGCCCAGTGCTCGCCGGAAGCCAGAATATGAGCCCATTGGC
  AGCACAGATGAAGCCCAGAAGAAGTTTGGCAACGTCAAGGCCATCTCTTCAGACATGTACTTTGGAATTC
  AAGCCCAGACAGATTTTGAAACCCGGGCGCGGCTGGAGAGGCTCTCCACCAGCTCCTCCATAAGCTCTGC
  AGACCTTTTTGATGAGCAGAGGAAGCAGACTGCAGGAAACTACAACCTTTCCAATGTGCTGCCCAATGCC
  CCAGACATGGCGCAGTTCAAGCAAGGTGTGCGCTCCGTTGCTGGAAAACTTTCCGTCTTCGCCAATGGGG
  TCATGACGTCCATTCAGGATCGCTACGGTTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208404 representing NM_025445
  Red=Cloning site Green=Tags(s)
  
  MGDPSKQDILAIFKRLRSVPTNKVCFDCGAKNPSWASISYGVFLCIDCSGSHRSLGVHLSFIRSTELDSN
  WSWFQLRCMQVGGNSNASSFFHQHGCATKDTNAKYNSRAAQLYREKIKTLATQATRRHGTDLWLDSCAAP
  PVSPPPKEEDFFASHASLEVSGAMQASAQPESASSTPWGLETTPEKHEGGPGQGPSVEGLNTPGKAAPEV
  SSIIKKKPNQAKKGLGAKKGSLGAQKLTNTSFTEIEKQAQAVDKRKEQEDLARGAPKEESIVSSLRLAYK
  DLEISRKKDERLNLSGQKKVEAERLGMGFGSCRSGISHSVTSDMQTIEQESPTLAKPRRKYQEDPEDSYF
  SSSSKWSEQSSSRYFDDPMELRSSSFSSWDDGADSYWKKDSSRDPEPAMRSTGSSDRPSARRKPEYEPIG
  STDEAQKKFGNVKAISSDMYFGIQAQTDFETRARLERLSTSSSISSADLFDEQRKQTAGNYNLSNVLPNA
  PDMAQFKQGVRSVAGKLSVFANGVMTSIQDRYGS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025445
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025445.2, NP_079721.2
• RefSeq Size: 2619 bp
• RefSeq ORF: 1575 bp
• Locus ID: 66251
• UniProt ID: Q9D8S3
• Cytogenetics: 15 E1
• Gene Summary: GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes (By similarity).[UniProtKB/Swiss-Prot Function]