Arfgap3 (NM_025445) Mouse Untagged Clone

CAT#: MC200353

Arfgap3 (untagged) - Mouse ADP-ribosylation factor GTPase activating protein 3 (Arfgap3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arfgap3
• Synonyms: 0610009H19Rik; 1810004P07Rik; 1810035F16Rik; 9130416J18Rik; Arfgap1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC004081 sequence for NM_025445
  GTCGGGGACTGCTTCACCTCAGTTCTCAGTGACTATGGGGGACCCCAGCAAGCAGGACATCCTGGCCATC TTCAAGCGCCTGCGCTCGGTGCCCACTAACAAGGTGTGTTTTGACTGTGGTGCCAAAAATCCCAGCTGGG CCAGCATAAGCTATGGGGTGTTTCTCTGCATTGACTGCTCTGGATCACACCGGTCGCTCGGGGTTCACTT GAGTTTTATTCGGTCCACAGAGCTGGATTCAAACTGGTCGTGGTTCCAGCTTCGATGCATGCAAGTTGGA GGGAACTCAAATGCTTCTTCCTTCTTCCACCAACATGGGTGTGCCACCAAGGACACCAACGCCAAGTACA ACAGCCGTGCGGCTCAGCTCTACAGGGAGAAGATCAAGACTCTGGCCACCCAGGCCACCCGCAGGCACGG CACCGATCTGTGGCTGGACAGCTGTGCGGCTCCACCTGTGTCCCCTCCTCCAAAGGAGGAAGATTTTTTT GCTTCTCACGCCTCTCTGGAGGTCAGTGGTGCTATGCAGGCATCCGCACAGCCAGAGTCAGCCTCTTCAA CACCGTGGGGCTTGGAAACCACTCCAGAAAAACATGAAGGTGGGCCAGGACAAGGGCCAAGCGTGGAAGG TCTGAACACCCCTGGAAAGGCTGCACCAGAGGTTTCATCGATTATAAAAAAGAAGCCAAATCAAGCAAAA AAAGGACTTGGAGCCAAAAAAGGAAGCTTGGGAGCCCAGAAACTGACAAATACATCCTTCACTGAGATCG AAAAGCAAGCTCAGGCTGTGGATAAGAGGAAGGAGCAGGAAGACCTTGCCAGGGGTGCCCCAAAGGAGGA GTCTATTGTTTCGTCCTTGCGACTGGCCTACAAGGATCTGGAAATCAGCAGGAAGAAAGATGAAAGACTG AACCTGAGTGGCCAGAAGAAGGTGGAAGCCGAGCGGCTGGGCATGGGCTTTGGAAGCTGCAGGAGTGGCA TCTCCCACTCGGTGACTTCAGACATGCAGACCATTGAGCAGGAGTCGCCCACACTGGCAAAGCCGAGGAG GAAGTACCAGGAGGACCCAGAGGACTCGTACTTCAGCTCCAGCTCCAAGTGGTCTGAGCAGAGCAGCAGC AGGTACTTTGACGACCCCATGGAGTTAAGAAGCAGCTCCTTCTCTAGCTGGGATGATGGTGCCGATTCCT ACTGGAAGAAAGACAGCAGCAGAGACCCCGAGCCTGCCATGAGAAGCACCGGCTCTTCAGACAGGCCCAG TGCTCGCCGGAAGCCAGAATATGAGCCCATTGGCAGCACAGATGAAGCCCAGAAGAAGTTTGGCAACGTC AAGGCCATCTCTTCAGACATGTACTTTGGAATTCAAGCCCAGACAGATTTTGAAACCCGGGCGCGGCTGG AGAGGCTCTCCACCAGCTCCTCCATAAGCTCTGCAGACCTTTTTGATGAGCAGAGGAAGCAGACTGCAGG AAACTACAACCTTTCCAATGTGCTGCCCAATGCCCCAGACATGGCGCAGTTCAAGCAAGGTGTGCGCTCC GTTGCTGGAAAACTTTCCGTCTTCGCCAATGGGGTCATGACGTCCATTCAGGATCGCTACGGTTCTTAAT CCTGGTGCCAGGCTGTCCAACCTGGTAAAACTCCACTTCAGATGGACCTGAGACCACATCTGGCTGCGCC AATGCCCCAGCTGCTTTGTGGGCAGGCTCTACTAGTTGGCGAGGCACCTGCACATTTCTGAGTGTCCATG TTTCCTTTGAGACATCCCCATTTCTATGTAGGTGGGATTTTGCTTTCATTTCTGATTTAAATTAGCTCCT TGTGTCCTTGCTTTATCTTTGGGGCATCTTTAGTTCCAAGAACTGGAAGCCAGAGCTGCAGGCTCCTCCC ACCTCTCTCCAGGCTGGTGTTGACTGGGCCCGGAAATCAATCCCCAGCACTTACTTCTCACTGCAAAGGA GCAAAACAACAAAAACAGCAAGACCAGAAGTGCCCAGCCCCTTGCTGACAGATTGCTTCGTGTGTGTCCC CACACAACACACACTTAAAGCCATCTGTCACTCTGTAAGTTATTTTGTACATTCTCTACTCTTAAAAGTG TCCCCCAGTCAGTTCCTTTAACAGACCTGAGCTTCTATGATGCCAAGGGCGTTCACTGCGACCTGGGAGT AAACAGGGTGTCGCTGCCCTGTGAGAGGAAGATGGGGCCACCCCAGGGCTCCCAATGCACTCGGAAGTCA GCAGTACCAGGAGCTCAGGCTGAGAAGGACAGCAGTGCGGCTTCAGGACGAAGCTCAGCCAGGGAGAGGG CGATTATAATTGGGTAGTTTTGTTAATATTTTTTTATCTTGTCCTTGATATTCCAAAAGTTACAGGATAG ATGTAAATCAATAAAGTCATTTGGCTGTGTTTTAGACAGCTCCAGAATATATTGTTCAGCGCAGTAAAAT ATATTTGGAATTTGATGAACCAAAAATGTGATTTCAGATGACTATTTTGTGACTTTCTTAAAAGGTCTGA TTTGTAGACTTCTAAATATAACACACAGCACAGCAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_025445
• Insert Size: 1575 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004081, AAH04081
• RefSeq Size: 2573 bp
• RefSeq ORF: 1575 bp
• Locus ID: 66251
• UniProt ID: Q9D8S3
• Cytogenetics: 15 E1
• Gene Summary: GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.