Cdc25a (NM_007658) Mouse Tagged ORF Clone

CAT#: MG208257

Cdc25a (tGFP-tagged) - Mouse cell division cycle 25 homolog A (S. pombe) (Cdc25a)

Product Images

Circular map for MG208257

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdc25a
• Synonyms: D9Ertd393e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208257 representing NM_007658
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGGGCCCGGAGCCCCCCCACCGCCGCCGCCTGTTCTTCGCTTGCAGCCCCACGCCTGCGCCGC
  AGCCCACGGGGAAGATGCTGTTTGGCGCGTCAGCTGCTGGCGGACTGTCCCCTGTCACCAACCTGACGGT
  CACCATGGACCAGCTGGAAGGACTGGGCAGTGACTGTGAGAAAATGGAAGTGAGAAATAACAGCAGTCTA
  CAGAGAATGGGCTCCTCAGAATCGACCGATTCAGGTTTCTGTCTAGATTCTCCTGGGCCCTTGGACAGTA
  AAGAAAACCTTGAAATTTCCCTGACGAGAATAAATTCCCTACCTCAGAAGCTCCTGGGATGTAGCCCAGC
  CCTGAAGAGGAGCCACTCTGATTCTCTAGACCACGACACCTTTCACCTCATCGACCAGGATGAAAATAAA
  GAAAATGAAGCATTTGAATTTAAAAAGCCAATACGACCTGCATCTCGTCACATCTACGAGGAGAGTAAAG
  ATCCCTTTACACACAGGCAGAATTCAGCCCCAGCTCGGATGCTATCTTCAAATGAAAGTGAATCAGGAAA
  TTTCAGTCCTCTTTTTATACCCCAGTCACCTGTAAAAGCCACTTTGTCCGATGAGGATGATGGCTTCATA
  GACCTTCTGGATGGCGAGAATATGAAGAATGATGAGGAGACCCCATCATGCATGGCAAGCCTCTGGACAG
  CTCCCCTTGTCATGAGGAGACCTGCAAACCTTGCCGATCGTTGCGGGCTGTTTGACTCCCCTTCCCCGTG
  CGGCTCCAGCACTCGGGCGGTGTTGAAGAGAGCAGACCGGTCTCACGAGGAGCCTCCTCGGGGTACAAAG
  AGGAGGAAGAGTGTGCCCAGCCCTGTGAAGGCGAAGGCGGATGTTCCGGAGCCCGCCCAGCTTCCATCCC
  AGTCTCTATCCCTGATGTCTTCCCCCAAAGGAACCATTGAGAACATTTTGGACAGTGACCCAAGAGACCT
  TATAGGAGATTTCTCCAAGGGTTATCTCTTTAATACAGTCTCTGGGAAGCATCAGGATTTGAAATATATT
  TCTCCAGAAATTATGGCATCTGTTTTGAATGGCAAGTTTGCCAGTCTCATTAAAGAGTTTGTTATCATTG
  ACTGCCGATACCCATATGAGTATGAAGGAGGTCACATCAAGGGTGCCGTGAATCTGCACATGGAAGAAGA
  GGTTGAAGACTTCTTACTTAAGAACCCTATTGTGCCTACTGATGGCAAGCGTGTCATTGTCGTGTTCCAC
  TGTGAGTTTTCCTCTGAAAGAGGCCCTCGAATGTGCCGATATGTGAGAGAGAGAGATAGGCTCGGCAATG
  AGTACCCCAAGCTCCACTACCCTGAGCTGTATGTCCTGAAGGGGGGATACAAGGAGTTCTTTTTGAAGTG
  CCAGTCTCACTGTGAACCCCCCAGCTACCGGCCAATGCACCATGAAGACTTTAAAGAAGACCTAAAGAAG
  TTCCGCACCAAGAGCCGGACCTGGGCAGGAGAAAAGAGCAAAAGAGAGATGTACAGTCGCCTGAAGAAGC
  TC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208257 representing NM_007658
  Red=Cloning site Green=Tags(s)
  
  MELGPEPPHRRRLFFACSPTPAPQPTGKMLFGASAAGGLSPVTNLTVTMDQLEGLGSDCEKMEVRNNSSL
  QRMGSSESTDSGFCLDSPGPLDSKENLEISLTRINSLPQKLLGCSPALKRSHSDSLDHDTFHLIDQDENK
  ENEAFEFKKPIRPASRHIYEESKDPFTHRQNSAPARMLSSNESESGNFSPLFIPQSPVKATLSDEDDGFI
  DLLDGENMKNDEETPSCMASLWTAPLVMRRPANLADRCGLFDSPSPCGSSTRAVLKRADRSHEEPPRGTK
  RRKSVPSPVKAKADVPEPAQLPSQSLSLMSSPKGTIENILDSDPRDLIGDFSKGYLFNTVSGKHQDLKYI
  SPEIMASVLNGKFASLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKNPIVPTDGKRVIVVFH
  CEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFLKCQSHCEPPSYRPMHHEDFKEDLKK
  FRTKSRTWAGEKSKREMYSRLKKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007658
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007658.2
• RefSeq Size: 3643 bp
• RefSeq ORF: 1545 bp
• Locus ID: 12530
• UniProt ID: P48964
• Cytogenetics: 9 59.81 cM
• Gene Summary: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro (By similarity). Phosphorylation by PIM1 leads to an increase in phosphatase activity (By similarity).[UniProtKB/Swiss-Prot Function]