Cdc25a (NM_007658) Mouse Untagged Clone

CAT#: MC204476

Cdc25a (untagged) - Mouse cell division cycle 25 homolog A (S. pombe) (Cdc25a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdc25a
• Synonyms: D9Ertd393e
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046296
  AGCGGGAGTCCGGGCAGGCCCGGCGGCGCCATGGAACTGGGCCCGGAGCCCCCCCACCGCCGCCGCCTGT TCTTCGCTTGCAGCCCCACGCCTGCGCCGCAGCCCACGGGGAAGATGCTGTTTGGCGCGTCAGCTGCTGG CGGACTGTCCCCTGTCACCAACCTGACGGTCACCATGGACCAGCTGGAAGGACTGGGCAGTGACTGTGAG AAAATGGAAGTGAGAAATAACAGCAGTCTACAGAGAATGGGCTCCTCAGAATCGACCGATTCAGGTTTCT GTCTAGATTCTCCTGGGCCCTTGGACAGTAAAGAAAACCTTGAAATTTCCCTGACGAGAATAAATTCCCT ACCTCAGAAGCTCCTGGGATGTAGCCCAGCCCTGAAGAGGAGCCACTCTGATTCTCTAGACCACGACACC TTTCACCTCATCGACCAGGATGAAAATAAAGAAAATGAAGCATTTGAATTTAAAAAGCCAATACGACCTG CATCTCGTCACATCTACGAGGAGAGTAAAGATCCCTTTACACACAGGCAGAATTCAGCCCCAGCTCGGAT GCTATCTTCAAATGAAAGTGAATCAGGAAATTTCAGTCCTCTTTTTATACCCCAGTCACCTGTAAAAGCC ACTTTGTCCGATGAGGATGATGGCTTCATAGACCTTCTGGATGGCGAGAATATGAAGAATGATGAGGAGA CCCCATCATGCATGGCAAGCCTCTGGACAGCTCCCCTTGTCATGAGGAGACCTGCAAACCTTGCCGATCG TTGCGGGCTGTTTGACTCCCCTTCCCCGTGCGGCTCCAGCACTCGGGCGGTGTTGAAGAGAGCAGACCGG TCTCACGAGGAGCCTCCTCGGGGTACAAAGAGGAGGAAGAGTGTGCCCAGCCCTGTGAAGGCGAAGGCGG ATGTTCCGGAGCCCGCCCAGCTTCCATCCCAGTCTCTATCCCTGATGTCTTCCCCCAAAGGAACCATTGA GAACATTTTGGACAGTGACCCAAGAGACCTTATAGGAGATTTCTCCAAGGGTTATCTCTTTAATACAGTC TCTGGGAAGCATCAGGATTTGAAATATATTTCTCCAGAAATTATGGCATCTGTTTTGAATGGCAAGTTTG CCAGTCTCATTAAAGAGTTTGTTATCATTGACTGCCGATACCCATATGAGTATGAAGGAGGTCACATCAA GGGTGCCGTGAATCTGCACATGGAAGAAGAGGTTGAAGACTTCTTACTTAAGAACCCTATTGTGCCTACT GATGGCAAGCGTGTCATTGTCGTGTTCCACTGTGAGTTTTCCTCTGAAAGAGGCCCTCGAATGTGCCGAT ATGTGAGAGAGAGAGATAGGCTCGGCAATGAGTACCCCAAGCTCCACTACCCTGAGCTGTATGTCCTGAA GGGGGGATACAAGGAGTTCTTTTTGAAGTGCCAGTCTCACTGTGAACCCCCCAGCTACCGGCCAATGCAC CATGAAGACTTTAAAGAAGACCTAAAGAAGTTCCGCACCAAGAGCCGGACCTGGGCAGGAGAAAAGAGCA AAAGAGAGATGTACAGTCGCCTGAAGAAGCTCTGAAGCCAAATGGTAGCAGCCTGAGCTTCCCTCTGCCC CGTCCCTTTTCCCTTTGCTGCAGAGCAGTAAGCAAAGGGGCCAGCTGTACGGTACCTGGAGAAAGACCTG GGCCTTCCATTCCTTGGACCTGTCTCCTACACTCCCAGGTTGGAGCCTAGGGCACCCTGCCGTTACACTC TTCTGTTCTGTAAGAGTCCTTCCCTCTCAGGACTGTCTGCCAAAGCTGGACAAGCACAGCACAGACTGGA GTCTGCAACACCGTGTTAGGCTGCTCTCACTGAGCATCCCATGAAGTACTCGGGCCTTATTGGGCAGACC CTGGCCTGTCACGAGGAGAGAAAAGCCGCCCAAGTGCTGCTGGCCAAATACCAAAGATAGGCTGGAAGGG AGGAGAGGGCCTCGTGGATGACTCTTAACTTTAATTTATTCAGCTTCATCAATTATTTTATTATTGTTTT AATTCAAGACTTTTACTTTTCTGCTTCATTAAGTCAAAATACTGCCATTCTAGGTAGAGTTTTATCATCC TAGGAGCTACCTCTACTTTTAATTTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007658
• Insert Size: 1545 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC046296, AAH46296
• RefSeq Size: 2140 bp
• RefSeq ORF: 1545 bp
• Locus ID: 12530
• UniProt ID: P48964
• Cytogenetics: 9 59.81 cM
• Gene Summary: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro (By similarity). Phosphorylation by PIM1 leads to an increase in phosphatase activity (By similarity).[UniProtKB/Swiss-Prot Function]