Fscn1 (NM_007984) Mouse Tagged ORF Clone

CAT#: MG207918

Fscn1 (tGFP-tagged) - Mouse fascin homolog 1, actin bundling protein (Strongylocentrotus purpuratus) (Fscn1)

Product Images

Circular map for MG207918

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fscn1
• Synonyms: AI663989; Fan1; fascin-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207918 representing NM_007984
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGCCAACGGCACGGCAGAGGCTGTGCAGATTCAGTTCGGGCTCATCAGCTGCGGCAACAAGTACC
  TGACAGCCGAGGCGTTCGGGTTCAAGGTGAACGCATCCGCTAGTAGCTTGAAAAAGAAGCAGATCTGGAC
  GCTGGAGCAACCTCCCGATGAGGCGGGCAGCGCGGCCGTGTGTCTGCGCAGCCACCTGGGTCGCTACCTG
  GCCGCCGACAAGGACGGCAACGTGACCTGCGAGCGCGAGGTGCCCGACGGCGACTGCCGCTTTCTCGTCG
  TGGCGCACGACGACGGCCGCTGGTCGCTGCAGTCCGAGGCTCACCGGCGCTACTTTGGCGGCACCGAGGA
  CCGCCTGTCCTGCTTCGCGCAGAGCGTGTCGCCGGCCGAGAAGTGGAGCGTGCACATCGCCATGCACCCG
  CAGGTTAACATCTACAGCGTTACCCGCAAGCGCTACGCGCATCTGAGCGCGCGGCCGGCCGACGAGATCG
  CGGTAGACCGCGACGTGCCTTGGGGCGTCGACTCGCTCATCACCTTGGCCTTCCAGGACCAACGCTACAG
  TGTGCAGACGTCCGACCACCGCTTCCTGCGCCACGACGGGCGCCTTGTGGCACGGCCGGAGCCCGCCACG
  GGCTTCACGCTGGAGTTCCGCTCCGGCAAGGTGGCCTTTCGCGACTGCGAAGGTCGCTACCTGGCTCCGT
  CCGGGCCCAGCGGCACCCTCAAGGCTGGCAAGGCCACCAAGGTGGGCAAAGATGAGCTCTTCGCCCTGGA
  ACAGAGCTGCGCTCAGGTGGTGCTGCAGGCGGCCAACGAGAGGAACGTGTCCACGCGCCAGGGAATGGAC
  CTGTCAGCCAATCAGGATGAAGAGACCGATCAGGAGACCTTCCAGCTGGAGATCGACCGCGACACAAGAA
  AGTGTGCCTTTCGCACCCACACGGGCAAGTACTGGACACTGACGGCGACCGGAGGTGTGCAATCCACTGC
  GTCCACCAAGAACGCCAGCTGCTACTTTGACATCGAGTGGTGTGACCGCCGGATCACTCTGAGAGCCTCC
  AACGGCAAGTTTGTGACCGCCAAGAAAAATGGCCAGCTGGCCGCCTCGGTGGAGACAGCAGGGGACTCGG
  AACTCTTCCTCATGAAGCTGATTAACCGCCCCATCATCGTGTTCCGGGGGGAACACGGGTTCATTGGCTG
  CCGCAAGGTCACGGGCACTCTGGATGCCAACCGTTCCAGTTACGATGTCTTCCAGTTGGAATTCAATGAC
  GGCGCCTACAACATCAAAGACTCCACGGGCAAGTACTGGACGGTGGGTAGTGATTCCTCGGTCACCAGCA
  GCAGCGACACCCCTGTGGATTTCTTCCTTGAGTTCTGTGACTACAATAAGGTGGCTCTCAAGGTGGGCGG
  CCGCTACCTGAAGGGGGACCACGCTGGGGTCCTGAAGGCCTGCGCGGAGACTATCGACCCCGCCTCACTC
  TGGGAGTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207918 representing NM_007984
  Red=Cloning site Green=Tags(s)
  
  MTANGTAEAVQIQFGLISCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLGRYL
  AADKDGNVTCEREVPDGDCRFLVVAHDDGRWSLQSEAHRRYFGGTEDRLSCFAQSVSPAEKWSVHIAMHP
  QVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTSDHRFLRHDGRLVARPEPAT
  GFTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMD
  LSANQDEETDQETFQLEIDRDTRKCAFRTHTGKYWTLTATGGVQSTASTKNASCYFDIEWCDRRITLRAS
  NGKFVTAKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFND
  GAYNIKDSTGKYWTVGSDSSVTSSSDTPVDFFLEFCDYNKVALKVGGRYLKGDHAGVLKACAETIDPASL
  WEY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007984
• ORF Size: 1479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007984.2, NP_032010.2
• RefSeq Size: 2667 bp
• RefSeq ORF: 1482 bp
• Locus ID: 14086
• UniProt ID: Q61553
• Cytogenetics: 5 81.84 cM
• Gene Summary: Actin-binding protein that contains 2 major actin binding sites (By similarity). Organizes filamentous actin into parallel bundles (PubMed:7738015). Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers (By similarity). Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration (PubMed:21685497). Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF (By similarity).[UniProtKB/Swiss-Prot Function]