Adam15 (BC009132) Mouse Tagged ORF Clone

CAT#: MG207150

Adam15 (tGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 15 (metargidin) (cDNA clone MGC:11753 IMAGE:3152945), complete

Product Images

Circular map for MG207150

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adam15
• Synonyms: MDC15, metargidin
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207150 representing BC009132
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCTGGCGCTGCTCTGGGCTCTGGGACTCCTGGGCGCGGGCAGCCCTCGGCCCTCCCCGCCGCTGC
  CAAATATAGGAGGCACTGAGGAAGAGCAGCAAGCCAGCCCAGAGAGGACGCTGAGTGGATCCATGGAGAG
  CCGGGTTGTTCAGGACAGCCCCCCAATGAGCCTAGCAGACGTGCTTCAGACTGGTTTACCTGAGGCCCTG
  AGGATTTCCTTGGAGCTGGACAGTGAGAGTCATGTCCTGGAGCTTCTACAAAATAGAGATCTAATCCCTG
  GCCGCCCAACTCTGGTGTGGTACCAGCCTGATGGCACCCGAATGGTCAGCGAGGGCTACAGTCTAGAAAA
  CTGCTGCTACCGAGGACGAGTGCAGGGCCACCCCAGCTCCTGGGTGTCCCTCTGTGCCTGCTCTGGGATC
  AGGGGGCTCATTGTCCTGTCCCCAGAGAGAGGCTATACACTGGAGCTGGGCCCTGGGGACCTTCAGCGTC
  CTGTCATTTCTCGGATCCAAGACCACCTGTTGCTGGGCCACACCTGTGCCCCAAGCTGGCATGCCTCTGT
  GCCCACTCGGGCAGGACCAGACCTCCTTCTGGAACAGCATCACGCTCACAGGCTTAAGCGAGATGTAGTA
  ACAGAGACGAAAATTGTGGAGTTGGTGATTGTGGCTGATAATTCAGAGGTCAGAAAGTACCCTGACTTCC
  AACAACTGCTGAACCGGACACTAGAAGCGGCTCTCTTGCTAGACACGTTCTTCCAGCCCTTGAATGTCCG
  GGTAGCCCTTGTGGGCCTAGAGGCATGGACCCAGCACAACCTGATAGAAATGAGCTCCAACCCAGCTGTC
  CTGCTAGACAACTTCCTCCGCTGGCGCCGGACAGACTTGCTGCCTCGACTGCCCCATGACAGTGCCCAAC
  TGGTGACTGTAACTTCCTTCTCTGGTCCCATGGTGGGCATGGCCATTCAGAATTCCATCTGTTCCCCTGA
  CTTCTCCGGAGGTGTGAATATGGACCACTCCACAAGCATCTTAGGCGTTGCCTCCTCGATTGCCCATGAA
  TTGGGCCACAGTCTGGGTTTGGACCATGATTCTCCCGGGCACAGCTGTCCCTGTCCAGGTCCAGCCCCGG
  CTAAGAGCTGCATCATGGAGGCCTCCACAGACTTCCTACCAGGTTTGAACTTCAGCAACTGCAGCCGACA
  GGCCCTGGAAAAGGCCCTCCTGGAAGGAATGGGCAGCTGCCTCTTCGAACGGCACCCACCAGGTGACCTG
  CCTGGCCCAGGAGATGGAAGCTTGCCGCTGGTGGTGCCCTCCAGGCCAGCTCCACCACCCCCTGCAGCAT
  CTTCGCTCTACCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207150 representing BC009132
  Red=Cloning site Green=Tags(s)
  
  MRLALLWALGLLGAGSPRPSPPLPNIGGTEEEQQASPERTLSGSMESRVVQDSPPMSLADVLQTGLPEAL
  RISLELDSESHVLELLQNRDLIPGRPTLVWYQPDGTRMVSEGYSLENCCYRGRVQGHPSSWVSLCACSGI
  RGLIVLSPERGYTLELGPGDLQRPVISRIQDHLLLGHTCAPSWHASVPTRAGPDLLLEQHHAHRLKRDVV
  TETKIVELVIVADNSEVRKYPDFQQLLNRTLEAALLLDTFFQPLNVRVALVGLEAWTQHNLIEMSSNPAV
  LLDNFLRWRRTDLLPRLPHDSAQLVTVTSFSGPMVGMAIQNSICSPDFSGGVNMDHSTSILGVASSIAHE
  LGHSLGLDHDSPGHSCPCPGPAPAKSCIMEASTDFLPGLNFSNCSRQALEKALLEGMGSCLFERHPPGDL
  PGPGDGSLPLVVPSRPAPPPPAASSLYL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC009132
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC009132.1
• RefSeq Size: 1704 bp
• RefSeq ORF: 1346 bp
• Locus ID: 11490
• Cytogenetics: 3 39.07 cM
• Gene Summary: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. This gene is prominently expressed in vascular cells, the endocardium, hypertrophic cells in developing bone, and specific areas of hippocampus and cerebellum. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. Mice lacking the encoded protein have increased bone mass resulting from osteoblast proliferation, and exhibit reduced neovascularization in a mouse model for retinopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing. [provided by RefSeq, May 2016]