Adam15 (BC009132) Mouse Untagged Clone

CAT#: MC200662

Adam15 (untagged) - Mouse a disintegrin and metallopeptidase domain 15 (metargidin) (cDNA clone MGC:11753 IMAGE:3152945), complete, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adam15
• Synonyms: MDC15, metargidin
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC009132
  GCGGGGCCGTGGCTGCTCCTCCACGCGTAGCCCCGCACCTGCTGCCCCAGTCCAGCCCGGAGCTCCGCGG CCATGCGGCTGGCGCTGCTCTGGGCTCTGGGACTCCTGGGCGCGGGCAGCCCTCGGCCCTCCCCGCCGCT GCCAAATATAGGAGGCACTGAGGAAGAGCAGCAAGCCAGCCCAGAGAGGACGCTGAGTGGATCCATGGAG AGCCGGGTTGTTCAGGACAGCCCCCCAATGAGCCTAGCAGACGTGCTTCAGACTGGTTTACCTGAGGCCC TGAGGATTTCCTTGGAGCTGGACAGTGAGAGTCATGTCCTGGAGCTTCTACAAAATAGAGATCTAATCCC TGGCCGCCCAACTCTGGTGTGGTACCAGCCTGATGGCACCCGAATGGTCAGCGAGGGCTACAGTCTAGAA AACTGCTGCTACCGAGGACGAGTGCAGGGCCACCCCAGCTCCTGGGTGTCCCTCTGTGCCTGCTCTGGGA TCAGGGGGCTCATTGTCCTGTCCCCAGAGAGAGGCTATACACTGGAGCTGGGCCCTGGGGACCTTCAGCG TCCTGTCATTTCTCGGATCCAAGACCACCTGTTGCTGGGCCACACCTGTGCCCCAAGCTGGCATGCCTCT GTGCCCACTCGGGCAGGACCAGACCTCCTTCTGGAACAGCATCACGCTCACAGGCTTAAGCGAGATGTAG TAACAGAGACGAAAATTGTGGAGTTGGTGATTGTGGCTGATAATTCAGAGGTCAGAAAGTACCCTGACTT CCAACAACTGCTGAACCGGACACTAGAAGCGGCTCTCTTGCTAGACACGTTCTTCCAGCCCTTGAATGTC CGGGTAGCCCTTGTGGGCCTAGAGGCATGGACCCAGCACAACCTGATAGAAATGAGCTCCAACCCAGCTG TCCTGCTAGACAACTTCCTCCGCTGGCGCCGGACAGACTTGCTGCCTCGACTGCCCCATGACAGTGCCCA ACTGGTGACTGTAACTTCCTTCTCTGGTCCCATGGTGGGCATGGCCATTCAGAATTCCATCTGTTCCCCT GACTTCTCCGGAGGTGTGAATATGGACCACTCCACAAGCATCTTAGGCGTTGCCTCCTCGATTGCCCATG AATTGGGCCACAGTCTGGGTTTGGACCATGATTCTCCCGGGCACAGCTGTCCCTGTCCAGGTCCAGCCCC GGCTAAGAGCTGCATCATGGAGGCCTCCACAGACTTCCTACCAGGTTTGAACTTCAGCAACTGCAGCCGA CAGGCCCTGGAAAAGGCCCTCCTGGAAGGAATGGGCAGCTGCCTCTTCGAACGGCACCCACCAGGTGACC TGCCTGGCCCAGGAGATGGAAGCTTGCCGCTGGTGGTGCCCTCCAGGCCAGCTCCACCACCCCCTGCAGC ATCTTCGCTCTACCTCTGACCTCTGGAGATTTGGCTGCCTCCTTCTCAAGCTCTAAGACTCAAAGAAATG GAACCTCTGCCCCAAACACTAGAGAAGCAGGAGAACAGACAATCTGGTGTCCAGCCCTAAAGAACCACCA GGCGCTGTTAAGCAATACCTGGGGACGCACTAAAATAGCTGCAGCGGGATGCTGGGGAGGGGCCGAAGCC GGGGCTGGAGTAGGTGGCGGAAGGACATGGACACTGTCTATCTCTGCTCTGTTGTAATAAATGTGAGATC TTGGAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC009132
• Insert Size: 1347 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC009132, AAH09132
• RefSeq Size: 1704 bp
• RefSeq ORF: 1347 bp
• Locus ID: 11490
• Cytogenetics: 3 39.07 cM
• Gene Summary: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. This gene is prominently expressed in vascular cells, the endocardium, hypertrophic cells in developing bone, and specific areas of hippocampus and cerebellum. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. Mice lacking the encoded protein have increased bone mass resulting from osteoblast proliferation, and exhibit reduced neovascularization in a mouse model for retinopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing. [provided by RefSeq, May 2016]