Tgtp1 (NM_011579) Mouse Tagged ORF Clone
CAT#: MG206553
- TrueORF®
Tgtp1 (tGFP-tagged) - Mouse T-cell specific GTPase (Tgtp)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_011579" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Tgtp1 |
Synonyms | Gtp2; Irgb6; Mg21; Tgtp |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206553 representing NM_011579
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGGGCCTCCAGCTTTGATGCATTCTTTAAGAATTTTAAAAGGGAAAGCAAAATCATCTCTGAAT ATGACATCACCTTGATTATGACTTACATAGAGGAAAATAAGCTACAGAAAGCTGTTTCTGTAATTGAAAA GGTACTGAGAGACATCGAGAGTGCTCCTCTGCACATAGCTGTGACAGGGGAAACAGGCGCAGGAAAGTCC ACTTTCATCAATACCCTGAGGGGGGTGGGGCATGAAGAAAAAGGTGCAGCCCCCACTGGCGCAATAGAGA CAACCATGAAGAGAACTCCATACCCACACCCAAAGCTTCCCAACGTGACAATATGGGACCTGCCTGGCAT TGGGACCACTAACTTCACACCACAAAACTATCTGACAGAAATGAAGTTTGGTGAGTACGACTTCTTCATT ATCATCTCAGCTACACGTTTCAAAGAAAATGATGCACAACTGGCCAAAGCCATTGCACAGATGGGGATGA ATTTCTACTTTGTCAGAACCAAGATAGACAGCGACTTAGATAATGAACAGAAGTTTAAGCCTAAGAGTTT CAATAAGGAGGAAGTCCTCAAGAATATTAAGGATTACTGCTCTAATCATCTTCAGGAGTCTCTCGACAGT GAGCCTCCAGTCTTCCTAGTCTCTAACGTTGATATATCGAAGTATGACTTCCCAAAGCTGGAAACTAAAC TCCTACAGGATCTCCCAGCCCACAAGCGCCACGTCTTCTCACTGTCTTTGCAAAGTCTTACTGAGGCCAC CATTAACTACAAGAGAGATTCCCTGAAGCAAAAAGTCTTCCTAGAAGCCATGAAGGCTGGAGCATTAGCC ACCATTCCACTTGGTGGCATGATCAGTGATATCTTAGAGAATCTGGATGAAACATTCAATCTCTACAGGT CTTACTTTGGGCTGGATGATGCTTCACTGGAAAACATTGCCCAGGATTTGAACATGTCTGTGGATGACTT CAAGGTACACCTTCGATTTCCCCATTTGTTTGCAGAACACAATGATGAGTCCTTAGAAGACAAGCTATTT AAATATATCAAACACATTTCTTCAGTTACTGGTGGGCCAGTCGCTGCAGTCACTTACTATCGCATGGCTT ATTATTTGCAGAATCTCTTTCTTGATACTGCAGCCAATGATGCCATAGCTCTTCTGAATAGTAAAGCACT TTTTGAGAAGAAGGTGGGACCATATATATCTGAGCCCCCCGAGTACTGGGAAGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206553 representing NM_011579
Red=Cloning site Green=Tags(s) MAWASSFDAFFKNFKRESKIISEYDITLIMTYIEENKLQKAVSVIEKVLRDIESAPLHIAVTGETGAGKS TFINTLRGVGHEEKGAAPTGAIETTMKRTPYPHPKLPNVTIWDLPGIGTTNFTPQNYLTEMKFGEYDFFI IISATRFKENDAQLAKAIAQMGMNFYFVRTKIDSDLDNEQKFKPKSFNKEEVLKNIKDYCSNHLQESLDS EPPVFLVSNVDISKYDFPKLETKLLQDLPAHKRHVFSLSLQSLTEATINYKRDSLKQKVFLEAMKAGALA TIPLGGMISDILENLDETFNLYRSYFGLDDASLENIAQDLNMSVDDFKVHLRFPHLFAEHNDESLEDKLF KYIKHISSVTGGPVAAVTYYRMAYYLQNLFLDTAANDAIALLNSKALFEKKVGPYISEPPEYWEA TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_011579 |
ORF Size | 1245 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011579.2, NP_035709.2 |
RefSeq Size | 2810 bp |
RefSeq ORF | 1248 bp |
Locus ID | 21822 |
UniProt ID | Q62293 |
Cytogenetics | 11 B1.2 |
Gene Summary | Involved in innate cell-autonomous resistance to intracellular pathogens, such as Toxoplasma gondii. During avirulent type II T. gondii infection, recruited to the parasitophorous vacuole (PV) membrane, leading to PV vesiculation and rupture, and subsequent digestion of the parasite within the cytosol (PubMed:19265156, PubMed:24563254). Not recruited to virulent type I T. gondii PV membrane (PubMed:19265156). May confer an antiviral state for vesicular stomatitis virus (PubMed:9725230).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208047 | Tgtp1 (untagged) - Mouse T-cell specific GTPase 1 (Tgtp1), (10ug) |
USD 503.00 |
|
MR206553 | Tgtp1 (Myc-DDK-tagged) - Mouse T-cell specific GTPase 1 (Tgtp1) |
USD 457.00 |
|
MR206553L3 | Lenti ORF clone of Tgtp1 (Myc-DDK-tagged) - Mouse T-cell specific GTPase 1 (Tgtp1) |
USD 757.00 |
|
MR206553L4 | Lenti ORF clone of Tgtp1 (mGFP-tagged) - Mouse T-cell specific GTPase 1 (Tgtp1) |
USD 757.00 |
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